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Endurance exercise and conjugated linoleic acid (CLA) supplementation up-regulate CYP17A1 and stimulate testosterone biosynthesis.

Barone R, Macaluso F, Catanese P, Marino Gammazza A, Rizzuto L, Marozzi P, Lo Giudice G, Stampone T, Cappello F, Morici G, Zummo G, Farina F, Di Felice V - PLoS ONE (2013)

Bottom Line: The effect of training on CYP17A1 expression and testosterone biosynthesis was significantly higher in the trained mice supplemented with CLA compared to the placebo.This effect was enhanced by CLA supplementation.Therefore, CLA-associated physical activity may be used for its steroidogenic property in different fields, such as alimentary industry, human reproductive medicine, sport science, and anti-muscle wasting.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Biomedicine and Clinical Neurosciences (BioNeC), University of Palermo, Palermo, Italy.

ABSTRACT
A new role for fat supplements, in particular conjugated linoleic acid (CLA), has been delineated in steroidogenesis, although the underlying molecular mechanisms have not yet been elucidated. The aims of the present study were to identify the pathway stimulated by CLA supplementation using a cell culture model and to determine whether this same pathway is also stimulated in vivo by CLA supplementation associated with exercise. In vitro, Leydig tumour rat cells (R2C) supplemented with different concentrations of CLA exhibited increasing testosterone biosynthesis accompanied by increasing levels of CYP17A1 mRNA and protein. In vivo, trained mice showed an increase in free plasma testosterone and an up-regulation of CYP17A1 mRNA and protein. The effect of training on CYP17A1 expression and testosterone biosynthesis was significantly higher in the trained mice supplemented with CLA compared to the placebo. The results of the present study demonstrated that CLA stimulates testosterone biosynthesis via CYP17A1, and endurance training led to the synthesis of testosterone in vivo by inducing the overexpression of CYP17A1 mRNA and protein in the Leydig cells of the testis. This effect was enhanced by CLA supplementation. Therefore, CLA-associated physical activity may be used for its steroidogenic property in different fields, such as alimentary industry, human reproductive medicine, sport science, and anti-muscle wasting.

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Effect of CLA supplementation on body weight, skeletal muscle, strength, and endurance performance invivo.A. Changes in body weight over time. B. Weight of the hindlimb anterior muscle groups (tibialis anterior, extensor hallucis longus, and extensor digitorum longus) normalised for body weight. C. Strength of the forelimb. D. Strength of the forelimb normalised to the body weight. E. Distance ran during the endurance test. SED, sedentary; TR, trained; PLA, placebo; CLA, conjugated linoleic acid. # Significant difference compared to time point “0” (P<0.05). ◊ Significant difference compared to the CLA-SED group (P<0.05). $ Significant difference compared to the PLA-SED group (P<0.05).
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pone-0079686-g004: Effect of CLA supplementation on body weight, skeletal muscle, strength, and endurance performance invivo.A. Changes in body weight over time. B. Weight of the hindlimb anterior muscle groups (tibialis anterior, extensor hallucis longus, and extensor digitorum longus) normalised for body weight. C. Strength of the forelimb. D. Strength of the forelimb normalised to the body weight. E. Distance ran during the endurance test. SED, sedentary; TR, trained; PLA, placebo; CLA, conjugated linoleic acid. # Significant difference compared to time point “0” (P<0.05). ◊ Significant difference compared to the CLA-SED group (P<0.05). $ Significant difference compared to the PLA-SED group (P<0.05).

Mentions: Body weight, strength, and endurance performance were measured to study the functional effects of endogenous testosterone stimulation by CLA supplementation and exercise. Moreover, to evaluate hypertrophy, the anterior muscle groups of the hindlimb were excised and weighed following euthanasia. Due to normal growth during the 6 weeks of experimentation, the mice had a physiological increase in body weight. The increased body weight was significant in all groups (P<0.05) except in the CLA-TR group. No significant difference was observed among the groups (Figure 4A). The training exercise and the CLA supplementation had an anabolic effect and increased the muscle mass of the hindlimbs (Figure 4B). The CLA-TR and PLA-TR groups presented with heavier anterior hindlimb muscle groups, after normalisation for body weight, compared to the CLA-SED and PLA-SED groups (P<0.05), respectively. When the strength data were not normalised for body weight, no significant difference was observed among the groups because of the large variability between the mice; however, only the CLA-TR group showed a significant increase after 6 weeks (P<0.05). When the strength data were normalised for body weight, the CLA-TR group had greater strength gains compared to the CLA-SED group (P<0.05) after 6 weeks. The strength data are summarised in Figure 4C and D. The CLA-TR group also showed an improvement in the endurance performance test compared to the CLA-SED group after 6 weeks (Figure 4E).


Endurance exercise and conjugated linoleic acid (CLA) supplementation up-regulate CYP17A1 and stimulate testosterone biosynthesis.

Barone R, Macaluso F, Catanese P, Marino Gammazza A, Rizzuto L, Marozzi P, Lo Giudice G, Stampone T, Cappello F, Morici G, Zummo G, Farina F, Di Felice V - PLoS ONE (2013)

Effect of CLA supplementation on body weight, skeletal muscle, strength, and endurance performance invivo.A. Changes in body weight over time. B. Weight of the hindlimb anterior muscle groups (tibialis anterior, extensor hallucis longus, and extensor digitorum longus) normalised for body weight. C. Strength of the forelimb. D. Strength of the forelimb normalised to the body weight. E. Distance ran during the endurance test. SED, sedentary; TR, trained; PLA, placebo; CLA, conjugated linoleic acid. # Significant difference compared to time point “0” (P<0.05). ◊ Significant difference compared to the CLA-SED group (P<0.05). $ Significant difference compared to the PLA-SED group (P<0.05).
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3818175&req=5

pone-0079686-g004: Effect of CLA supplementation on body weight, skeletal muscle, strength, and endurance performance invivo.A. Changes in body weight over time. B. Weight of the hindlimb anterior muscle groups (tibialis anterior, extensor hallucis longus, and extensor digitorum longus) normalised for body weight. C. Strength of the forelimb. D. Strength of the forelimb normalised to the body weight. E. Distance ran during the endurance test. SED, sedentary; TR, trained; PLA, placebo; CLA, conjugated linoleic acid. # Significant difference compared to time point “0” (P<0.05). ◊ Significant difference compared to the CLA-SED group (P<0.05). $ Significant difference compared to the PLA-SED group (P<0.05).
Mentions: Body weight, strength, and endurance performance were measured to study the functional effects of endogenous testosterone stimulation by CLA supplementation and exercise. Moreover, to evaluate hypertrophy, the anterior muscle groups of the hindlimb were excised and weighed following euthanasia. Due to normal growth during the 6 weeks of experimentation, the mice had a physiological increase in body weight. The increased body weight was significant in all groups (P<0.05) except in the CLA-TR group. No significant difference was observed among the groups (Figure 4A). The training exercise and the CLA supplementation had an anabolic effect and increased the muscle mass of the hindlimbs (Figure 4B). The CLA-TR and PLA-TR groups presented with heavier anterior hindlimb muscle groups, after normalisation for body weight, compared to the CLA-SED and PLA-SED groups (P<0.05), respectively. When the strength data were not normalised for body weight, no significant difference was observed among the groups because of the large variability between the mice; however, only the CLA-TR group showed a significant increase after 6 weeks (P<0.05). When the strength data were normalised for body weight, the CLA-TR group had greater strength gains compared to the CLA-SED group (P<0.05) after 6 weeks. The strength data are summarised in Figure 4C and D. The CLA-TR group also showed an improvement in the endurance performance test compared to the CLA-SED group after 6 weeks (Figure 4E).

Bottom Line: The effect of training on CYP17A1 expression and testosterone biosynthesis was significantly higher in the trained mice supplemented with CLA compared to the placebo.This effect was enhanced by CLA supplementation.Therefore, CLA-associated physical activity may be used for its steroidogenic property in different fields, such as alimentary industry, human reproductive medicine, sport science, and anti-muscle wasting.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Biomedicine and Clinical Neurosciences (BioNeC), University of Palermo, Palermo, Italy.

ABSTRACT
A new role for fat supplements, in particular conjugated linoleic acid (CLA), has been delineated in steroidogenesis, although the underlying molecular mechanisms have not yet been elucidated. The aims of the present study were to identify the pathway stimulated by CLA supplementation using a cell culture model and to determine whether this same pathway is also stimulated in vivo by CLA supplementation associated with exercise. In vitro, Leydig tumour rat cells (R2C) supplemented with different concentrations of CLA exhibited increasing testosterone biosynthesis accompanied by increasing levels of CYP17A1 mRNA and protein. In vivo, trained mice showed an increase in free plasma testosterone and an up-regulation of CYP17A1 mRNA and protein. The effect of training on CYP17A1 expression and testosterone biosynthesis was significantly higher in the trained mice supplemented with CLA compared to the placebo. The results of the present study demonstrated that CLA stimulates testosterone biosynthesis via CYP17A1, and endurance training led to the synthesis of testosterone in vivo by inducing the overexpression of CYP17A1 mRNA and protein in the Leydig cells of the testis. This effect was enhanced by CLA supplementation. Therefore, CLA-associated physical activity may be used for its steroidogenic property in different fields, such as alimentary industry, human reproductive medicine, sport science, and anti-muscle wasting.

Show MeSH
Related in: MedlinePlus