Limits...
Endurance exercise and conjugated linoleic acid (CLA) supplementation up-regulate CYP17A1 and stimulate testosterone biosynthesis.

Barone R, Macaluso F, Catanese P, Marino Gammazza A, Rizzuto L, Marozzi P, Lo Giudice G, Stampone T, Cappello F, Morici G, Zummo G, Farina F, Di Felice V - PLoS ONE (2013)

Bottom Line: The effect of training on CYP17A1 expression and testosterone biosynthesis was significantly higher in the trained mice supplemented with CLA compared to the placebo.This effect was enhanced by CLA supplementation.Therefore, CLA-associated physical activity may be used for its steroidogenic property in different fields, such as alimentary industry, human reproductive medicine, sport science, and anti-muscle wasting.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Biomedicine and Clinical Neurosciences (BioNeC), University of Palermo, Palermo, Italy.

ABSTRACT
A new role for fat supplements, in particular conjugated linoleic acid (CLA), has been delineated in steroidogenesis, although the underlying molecular mechanisms have not yet been elucidated. The aims of the present study were to identify the pathway stimulated by CLA supplementation using a cell culture model and to determine whether this same pathway is also stimulated in vivo by CLA supplementation associated with exercise. In vitro, Leydig tumour rat cells (R2C) supplemented with different concentrations of CLA exhibited increasing testosterone biosynthesis accompanied by increasing levels of CYP17A1 mRNA and protein. In vivo, trained mice showed an increase in free plasma testosterone and an up-regulation of CYP17A1 mRNA and protein. The effect of training on CYP17A1 expression and testosterone biosynthesis was significantly higher in the trained mice supplemented with CLA compared to the placebo. The results of the present study demonstrated that CLA stimulates testosterone biosynthesis via CYP17A1, and endurance training led to the synthesis of testosterone in vivo by inducing the overexpression of CYP17A1 mRNA and protein in the Leydig cells of the testis. This effect was enhanced by CLA supplementation. Therefore, CLA-associated physical activity may be used for its steroidogenic property in different fields, such as alimentary industry, human reproductive medicine, sport science, and anti-muscle wasting.

Show MeSH

Related in: MedlinePlus

Effect of CLA supplementation on steroidogenic genes and proteins invivo.A. Real-time PCR analysis of genes encoding steroidogenic enzymes from invivo testicular extracts. The graph shows the normalisation with the reference genes, according to the Livak Method (2-∆∆CT). B. Representative western blots showing CYP17A1 (55 kDa) and ß-actin (42 kDa) expression following electrophoretic separation of protein extracts obtained from testicles. C. Relative amounts of CYP17A1. SED, sedentary; TR, trained; PLA, placebo; CLA, conjugated linoleic acid. ∆ significant difference compared to the PLA-SED and CLA-SED groups (P<0.01). † Significant difference compared to the PLA-TR group (P<0.01).
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3818175&req=5

pone-0079686-g003: Effect of CLA supplementation on steroidogenic genes and proteins invivo.A. Real-time PCR analysis of genes encoding steroidogenic enzymes from invivo testicular extracts. The graph shows the normalisation with the reference genes, according to the Livak Method (2-∆∆CT). B. Representative western blots showing CYP17A1 (55 kDa) and ß-actin (42 kDa) expression following electrophoretic separation of protein extracts obtained from testicles. C. Relative amounts of CYP17A1. SED, sedentary; TR, trained; PLA, placebo; CLA, conjugated linoleic acid. ∆ significant difference compared to the PLA-SED and CLA-SED groups (P<0.01). † Significant difference compared to the PLA-TR group (P<0.01).

Mentions: The mRNA extracted from the testicles of mice under the four different conditions were analysed using real-time quantitative qPCR to detect whether specific genes encoding enzymes involved in the conversion of cholesterol to testosterone were up-regulated (Figure 3A). Data analysis revealed an up-regulation of CYP17A1 mRNA in the CLA-TR group (P<0.01). The expression of STAR, CYP11A1, and HSD3B1 mRNA did not change under the different supplementation conditions (PLA and CLA) or in response to physical activity (SED and TR).


Endurance exercise and conjugated linoleic acid (CLA) supplementation up-regulate CYP17A1 and stimulate testosterone biosynthesis.

Barone R, Macaluso F, Catanese P, Marino Gammazza A, Rizzuto L, Marozzi P, Lo Giudice G, Stampone T, Cappello F, Morici G, Zummo G, Farina F, Di Felice V - PLoS ONE (2013)

Effect of CLA supplementation on steroidogenic genes and proteins invivo.A. Real-time PCR analysis of genes encoding steroidogenic enzymes from invivo testicular extracts. The graph shows the normalisation with the reference genes, according to the Livak Method (2-∆∆CT). B. Representative western blots showing CYP17A1 (55 kDa) and ß-actin (42 kDa) expression following electrophoretic separation of protein extracts obtained from testicles. C. Relative amounts of CYP17A1. SED, sedentary; TR, trained; PLA, placebo; CLA, conjugated linoleic acid. ∆ significant difference compared to the PLA-SED and CLA-SED groups (P<0.01). † Significant difference compared to the PLA-TR group (P<0.01).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3818175&req=5

pone-0079686-g003: Effect of CLA supplementation on steroidogenic genes and proteins invivo.A. Real-time PCR analysis of genes encoding steroidogenic enzymes from invivo testicular extracts. The graph shows the normalisation with the reference genes, according to the Livak Method (2-∆∆CT). B. Representative western blots showing CYP17A1 (55 kDa) and ß-actin (42 kDa) expression following electrophoretic separation of protein extracts obtained from testicles. C. Relative amounts of CYP17A1. SED, sedentary; TR, trained; PLA, placebo; CLA, conjugated linoleic acid. ∆ significant difference compared to the PLA-SED and CLA-SED groups (P<0.01). † Significant difference compared to the PLA-TR group (P<0.01).
Mentions: The mRNA extracted from the testicles of mice under the four different conditions were analysed using real-time quantitative qPCR to detect whether specific genes encoding enzymes involved in the conversion of cholesterol to testosterone were up-regulated (Figure 3A). Data analysis revealed an up-regulation of CYP17A1 mRNA in the CLA-TR group (P<0.01). The expression of STAR, CYP11A1, and HSD3B1 mRNA did not change under the different supplementation conditions (PLA and CLA) or in response to physical activity (SED and TR).

Bottom Line: The effect of training on CYP17A1 expression and testosterone biosynthesis was significantly higher in the trained mice supplemented with CLA compared to the placebo.This effect was enhanced by CLA supplementation.Therefore, CLA-associated physical activity may be used for its steroidogenic property in different fields, such as alimentary industry, human reproductive medicine, sport science, and anti-muscle wasting.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Biomedicine and Clinical Neurosciences (BioNeC), University of Palermo, Palermo, Italy.

ABSTRACT
A new role for fat supplements, in particular conjugated linoleic acid (CLA), has been delineated in steroidogenesis, although the underlying molecular mechanisms have not yet been elucidated. The aims of the present study were to identify the pathway stimulated by CLA supplementation using a cell culture model and to determine whether this same pathway is also stimulated in vivo by CLA supplementation associated with exercise. In vitro, Leydig tumour rat cells (R2C) supplemented with different concentrations of CLA exhibited increasing testosterone biosynthesis accompanied by increasing levels of CYP17A1 mRNA and protein. In vivo, trained mice showed an increase in free plasma testosterone and an up-regulation of CYP17A1 mRNA and protein. The effect of training on CYP17A1 expression and testosterone biosynthesis was significantly higher in the trained mice supplemented with CLA compared to the placebo. The results of the present study demonstrated that CLA stimulates testosterone biosynthesis via CYP17A1, and endurance training led to the synthesis of testosterone in vivo by inducing the overexpression of CYP17A1 mRNA and protein in the Leydig cells of the testis. This effect was enhanced by CLA supplementation. Therefore, CLA-associated physical activity may be used for its steroidogenic property in different fields, such as alimentary industry, human reproductive medicine, sport science, and anti-muscle wasting.

Show MeSH
Related in: MedlinePlus