Limits...
Angiotensin II Stimulates Sympathetic Neurotransmission to Adipose Tissue.

King VL, English VL, Bharadwaj K, Cassis LA - Physiol Rep (2013)

Bottom Line: These effects of AngII contribute to cardiovascular control.At doses that lowered body weight, AngII significantly increased ISBAT [(3)H]NIS binding density.AngII significantly increased the rate of NE decline in all tissues compared to saline.

View Article: PubMed Central - PubMed

Affiliation: Division of Cardiology, University of Kentucky, Lexington, KY 40536.

ABSTRACT
Angiotensin II (AngII) facilitates sympathetic neurotransmission by regulating norepinephrine (NE) synthesis, release and uptake. These effects of AngII contribute to cardiovascular control. Previous studies in our laboratory demonstrated that chronic AngII infusion decreased body weight of rats. We hypothesized that AngII facilitates sympathetic neurotransmission to adipose tissue and may thereby decrease body weight. The effect of chronic AngII infusion on the NE uptake transporter and NE turnover was examined in metabolic (interscapular brown adipose tissue, ISBAT; epididymal fat, EF) and cardiovascular tissues (left ventricle, LV; kidney) of rats. To examine the uptake transporter saturation isotherms were performed using [(3)H]nisoxetine (NIS). At doses that lowered body weight, AngII significantly increased ISBAT [(3)H]NIS binding density. To quantify NE turnover, alpha-methyl-para-tyrosine (AMPT) was injected in saline-infused, AngII-infused, or saline-infused rats that were pair-fed to food intake of AngII-infused rats. AngII significantly increased the rate of NE decline in all tissues compared to saline. The rate of NE decline in EF was increased to a similar extent by AngII and by pair-feeding. In rats administered AngII and propranolol, reductions in food and water intake and body weight were eliminated. These data support the hypothesis that AngII facilitates sympathetic neurotransmission to adipose tissue. Increased sympathetic neurotransmission to adipose tissue following AngII exposure is suggested to contribute to reductions in body weight.

No MeSH data available.


Related in: MedlinePlus

Chronic angiotensin II (AngII) infusion increases plasma norepinephrine (NE concentration. AngII (200–600 ng/kg per minute) or saline were infused for 14 days by osmotic minipump. NE in plasma was extracted over alumina, followed by high-performance liquid chromatography (HPLC) with electrochemical detection for separation and quantification. Data are mean ± SEM from n = 8 rats/group; “*” denotes significantly different from control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3818081&req=5

fig02: Chronic angiotensin II (AngII) infusion increases plasma norepinephrine (NE concentration. AngII (200–600 ng/kg per minute) or saline were infused for 14 days by osmotic minipump. NE in plasma was extracted over alumina, followed by high-performance liquid chromatography (HPLC) with electrochemical detection for separation and quantification. Data are mean ± SEM from n = 8 rats/group; “*” denotes significantly different from control.

Mentions: Systolic blood pressure was significantly increased by AngII infusion (Table 1). Plasma NE concentration was significantly increased in rats infused with all doses of AngII compared to control (Fig. 2). However, the magnitude of the increase in plasma NE concentration was greatest at the lowest dose of AngII (200 ng/kg per minute).


Angiotensin II Stimulates Sympathetic Neurotransmission to Adipose Tissue.

King VL, English VL, Bharadwaj K, Cassis LA - Physiol Rep (2013)

Chronic angiotensin II (AngII) infusion increases plasma norepinephrine (NE concentration. AngII (200–600 ng/kg per minute) or saline were infused for 14 days by osmotic minipump. NE in plasma was extracted over alumina, followed by high-performance liquid chromatography (HPLC) with electrochemical detection for separation and quantification. Data are mean ± SEM from n = 8 rats/group; “*” denotes significantly different from control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3818081&req=5

fig02: Chronic angiotensin II (AngII) infusion increases plasma norepinephrine (NE concentration. AngII (200–600 ng/kg per minute) or saline were infused for 14 days by osmotic minipump. NE in plasma was extracted over alumina, followed by high-performance liquid chromatography (HPLC) with electrochemical detection for separation and quantification. Data are mean ± SEM from n = 8 rats/group; “*” denotes significantly different from control.
Mentions: Systolic blood pressure was significantly increased by AngII infusion (Table 1). Plasma NE concentration was significantly increased in rats infused with all doses of AngII compared to control (Fig. 2). However, the magnitude of the increase in plasma NE concentration was greatest at the lowest dose of AngII (200 ng/kg per minute).

Bottom Line: These effects of AngII contribute to cardiovascular control.At doses that lowered body weight, AngII significantly increased ISBAT [(3)H]NIS binding density.AngII significantly increased the rate of NE decline in all tissues compared to saline.

View Article: PubMed Central - PubMed

Affiliation: Division of Cardiology, University of Kentucky, Lexington, KY 40536.

ABSTRACT
Angiotensin II (AngII) facilitates sympathetic neurotransmission by regulating norepinephrine (NE) synthesis, release and uptake. These effects of AngII contribute to cardiovascular control. Previous studies in our laboratory demonstrated that chronic AngII infusion decreased body weight of rats. We hypothesized that AngII facilitates sympathetic neurotransmission to adipose tissue and may thereby decrease body weight. The effect of chronic AngII infusion on the NE uptake transporter and NE turnover was examined in metabolic (interscapular brown adipose tissue, ISBAT; epididymal fat, EF) and cardiovascular tissues (left ventricle, LV; kidney) of rats. To examine the uptake transporter saturation isotherms were performed using [(3)H]nisoxetine (NIS). At doses that lowered body weight, AngII significantly increased ISBAT [(3)H]NIS binding density. To quantify NE turnover, alpha-methyl-para-tyrosine (AMPT) was injected in saline-infused, AngII-infused, or saline-infused rats that were pair-fed to food intake of AngII-infused rats. AngII significantly increased the rate of NE decline in all tissues compared to saline. The rate of NE decline in EF was increased to a similar extent by AngII and by pair-feeding. In rats administered AngII and propranolol, reductions in food and water intake and body weight were eliminated. These data support the hypothesis that AngII facilitates sympathetic neurotransmission to adipose tissue. Increased sympathetic neurotransmission to adipose tissue following AngII exposure is suggested to contribute to reductions in body weight.

No MeSH data available.


Related in: MedlinePlus