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Silver nanoparticle/chitosan oligosaccharide/poly(vinyl alcohol) nanofibers as wound dressings: a preclinical study.

Li C, Fu R, Yu C, Li Z, Guan H, Hu D, Zhao D, Lu L - Int J Nanomedicine (2013)

Bottom Line: The AgNPs were reduced by COS prior to electrospinning or Ag(+) was reduced via ultraviolet irradiation in nanofibers.The nanofibers significantly inhibited growth of Escherichia coli and Staphylococcus aureus bacteria.The results of our in vitro and in vivo animal experiments suggest that PVA/COS-AgNP nanofibers should be of greater interest than PVA/COS/AgNO3 nanofibers for clinical use as a bioactive wound dressing.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, People's Republic of China.

ABSTRACT
In this study, a mixture of poly(vinyl alcohol) (PVA) and chitosan oligosaccharides (COS) was electrospun with silver nanoparticles (AgNPs) to produce fibrous mats for use in wound healing. The AgNPs were reduced by COS prior to electrospinning or Ag(+) was reduced via ultraviolet irradiation in nanofibers. The morphologies of the PVA/COS/AgNO3 and PVA/COS-AgNP nanofibers were analyzed by scanning electron microscopy. Formation of the AgNPs was investigated by field emission transmission electron microscopy, ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction. We also evaluated the biocompatibility of the nanofibers, particularly their cytotoxicity to human skin fibroblasts and potential to cause primary skin irritation. The in vitro antibacterial activity and in vivo wound healing capacity of the nanofibers were also investigated. The nanofibers had a smooth surface with an average diameter of 130-192 nm. The diameters of the AgNPs were in the range of 15-22 nm. The nanofibers significantly inhibited growth of Escherichia coli and Staphylococcus aureus bacteria. PVA/COS-AgNP nanofibers accelerated the rate of wound healing over that of the control (gauze). The results of our in vitro and in vivo animal experiments suggest that PVA/COS-AgNP nanofibers should be of greater interest than PVA/COS/AgNO3 nanofibers for clinical use as a bioactive wound dressing.

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Cytotoxicity tests from the MTT assays of cell viability. Absorbance was normalized to that of the negative control at each time interval, and was considered 100%.Notes: *P<0.05 compared with the negative control. The data are presented as the mean ± standard deviation (n=5).Abbreviations: AgNP, silver nanoparticle; COS, chitosan oligosaccharide; PVA, poly(vinyl alcohol).
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f9-ijn-8-4131: Cytotoxicity tests from the MTT assays of cell viability. Absorbance was normalized to that of the negative control at each time interval, and was considered 100%.Notes: *P<0.05 compared with the negative control. The data are presented as the mean ± standard deviation (n=5).Abbreviations: AgNP, silver nanoparticle; COS, chitosan oligosaccharide; PVA, poly(vinyl alcohol).

Mentions: The cytotoxicity of various concentrations of the extraction medium from the neat PVA/COS, PVA/COS/AgNO3, and PVA/COS-AgNP nanofiber mats is shown in Figure 9. Cell viability decreased significantly when human skin fibroblasts were incubated with higher concentrations (0.8–1.0 mg/mL) of the extraction medium from the PVA/COS/AgNO3 nanofiber mat when compared with the control (P<0.05). However, no significant cytotoxicity was observed at any concentration with the neat PVA/COS and PVA/COS-AgNP nanofiber mats. These data suggest that the PVA/COS-AgNP nanofiber mat has excellent in vitro biocompatibility and has marked benefits over the PVA/COS/AgNO3 nanofiber mat at the concentrations tested (0.8–1.0 mg/mL).


Silver nanoparticle/chitosan oligosaccharide/poly(vinyl alcohol) nanofibers as wound dressings: a preclinical study.

Li C, Fu R, Yu C, Li Z, Guan H, Hu D, Zhao D, Lu L - Int J Nanomedicine (2013)

Cytotoxicity tests from the MTT assays of cell viability. Absorbance was normalized to that of the negative control at each time interval, and was considered 100%.Notes: *P<0.05 compared with the negative control. The data are presented as the mean ± standard deviation (n=5).Abbreviations: AgNP, silver nanoparticle; COS, chitosan oligosaccharide; PVA, poly(vinyl alcohol).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3818021&req=5

f9-ijn-8-4131: Cytotoxicity tests from the MTT assays of cell viability. Absorbance was normalized to that of the negative control at each time interval, and was considered 100%.Notes: *P<0.05 compared with the negative control. The data are presented as the mean ± standard deviation (n=5).Abbreviations: AgNP, silver nanoparticle; COS, chitosan oligosaccharide; PVA, poly(vinyl alcohol).
Mentions: The cytotoxicity of various concentrations of the extraction medium from the neat PVA/COS, PVA/COS/AgNO3, and PVA/COS-AgNP nanofiber mats is shown in Figure 9. Cell viability decreased significantly when human skin fibroblasts were incubated with higher concentrations (0.8–1.0 mg/mL) of the extraction medium from the PVA/COS/AgNO3 nanofiber mat when compared with the control (P<0.05). However, no significant cytotoxicity was observed at any concentration with the neat PVA/COS and PVA/COS-AgNP nanofiber mats. These data suggest that the PVA/COS-AgNP nanofiber mat has excellent in vitro biocompatibility and has marked benefits over the PVA/COS/AgNO3 nanofiber mat at the concentrations tested (0.8–1.0 mg/mL).

Bottom Line: The AgNPs were reduced by COS prior to electrospinning or Ag(+) was reduced via ultraviolet irradiation in nanofibers.The nanofibers significantly inhibited growth of Escherichia coli and Staphylococcus aureus bacteria.The results of our in vitro and in vivo animal experiments suggest that PVA/COS-AgNP nanofibers should be of greater interest than PVA/COS/AgNO3 nanofibers for clinical use as a bioactive wound dressing.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, People's Republic of China.

ABSTRACT
In this study, a mixture of poly(vinyl alcohol) (PVA) and chitosan oligosaccharides (COS) was electrospun with silver nanoparticles (AgNPs) to produce fibrous mats for use in wound healing. The AgNPs were reduced by COS prior to electrospinning or Ag(+) was reduced via ultraviolet irradiation in nanofibers. The morphologies of the PVA/COS/AgNO3 and PVA/COS-AgNP nanofibers were analyzed by scanning electron microscopy. Formation of the AgNPs was investigated by field emission transmission electron microscopy, ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction. We also evaluated the biocompatibility of the nanofibers, particularly their cytotoxicity to human skin fibroblasts and potential to cause primary skin irritation. The in vitro antibacterial activity and in vivo wound healing capacity of the nanofibers were also investigated. The nanofibers had a smooth surface with an average diameter of 130-192 nm. The diameters of the AgNPs were in the range of 15-22 nm. The nanofibers significantly inhibited growth of Escherichia coli and Staphylococcus aureus bacteria. PVA/COS-AgNP nanofibers accelerated the rate of wound healing over that of the control (gauze). The results of our in vitro and in vivo animal experiments suggest that PVA/COS-AgNP nanofibers should be of greater interest than PVA/COS/AgNO3 nanofibers for clinical use as a bioactive wound dressing.

Show MeSH
Related in: MedlinePlus