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Silver nanoparticle/chitosan oligosaccharide/poly(vinyl alcohol) nanofibers as wound dressings: a preclinical study.

Li C, Fu R, Yu C, Li Z, Guan H, Hu D, Zhao D, Lu L - Int J Nanomedicine (2013)

Bottom Line: The AgNPs were reduced by COS prior to electrospinning or Ag(+) was reduced via ultraviolet irradiation in nanofibers.The nanofibers significantly inhibited growth of Escherichia coli and Staphylococcus aureus bacteria.The results of our in vitro and in vivo animal experiments suggest that PVA/COS-AgNP nanofibers should be of greater interest than PVA/COS/AgNO3 nanofibers for clinical use as a bioactive wound dressing.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, People's Republic of China.

ABSTRACT
In this study, a mixture of poly(vinyl alcohol) (PVA) and chitosan oligosaccharides (COS) was electrospun with silver nanoparticles (AgNPs) to produce fibrous mats for use in wound healing. The AgNPs were reduced by COS prior to electrospinning or Ag(+) was reduced via ultraviolet irradiation in nanofibers. The morphologies of the PVA/COS/AgNO3 and PVA/COS-AgNP nanofibers were analyzed by scanning electron microscopy. Formation of the AgNPs was investigated by field emission transmission electron microscopy, ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction. We also evaluated the biocompatibility of the nanofibers, particularly their cytotoxicity to human skin fibroblasts and potential to cause primary skin irritation. The in vitro antibacterial activity and in vivo wound healing capacity of the nanofibers were also investigated. The nanofibers had a smooth surface with an average diameter of 130-192 nm. The diameters of the AgNPs were in the range of 15-22 nm. The nanofibers significantly inhibited growth of Escherichia coli and Staphylococcus aureus bacteria. PVA/COS-AgNP nanofibers accelerated the rate of wound healing over that of the control (gauze). The results of our in vitro and in vivo animal experiments suggest that PVA/COS-AgNP nanofibers should be of greater interest than PVA/COS/AgNO3 nanofibers for clinical use as a bioactive wound dressing.

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Fourier transform infrared spectra of (A) the neat PVA/COS nanofiber, (B) the PVA/COS/AgNO3 nanofiber, and (C) the PVA/COS-AgNP nanofiber.Abbreviations: AgNP, silver nanoparticle; COS, chitosan oligosaccharide; PVA, poly(vinyl alcohol).
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f4-ijn-8-4131: Fourier transform infrared spectra of (A) the neat PVA/COS nanofiber, (B) the PVA/COS/AgNO3 nanofiber, and (C) the PVA/COS-AgNP nanofiber.Abbreviations: AgNP, silver nanoparticle; COS, chitosan oligosaccharide; PVA, poly(vinyl alcohol).

Mentions: The Fourier transform infrared spectra for the PVA/COS, PVA/COS/AgNO3, and PVA/COS-AgNP nanofibers are shown in Figure 4. The characteristic peaks of the neat PVA/COS nanofibers were assigned as follows: 3,308 cm−1 (N-H group), 2,942 cm−1 (CH2 asymmetric vibration), and 1,715 and 1,085 cm−1 (C=O bond). The peak at 1,248 cm−1 was associated with C=O=C vibrations and indicates the cross-linking of some PVA radicals.21 For the PVA/COS and PVA/COS/AgNO3 mats, the two spectra were similar with no shifts in any of the peaks, suggesting that the AgNPs were physically entrapped in the scaffold by van der Waals forces. Similar results have been reported by others.22,23 A significant change in the shape of the band at 3,338 cm−1 was observed between the PVA/COS nanofibers and the PVA/COS-AgNP nanofibers, indicating that N-H vibration was affected by attachment of the AgNPs.


Silver nanoparticle/chitosan oligosaccharide/poly(vinyl alcohol) nanofibers as wound dressings: a preclinical study.

Li C, Fu R, Yu C, Li Z, Guan H, Hu D, Zhao D, Lu L - Int J Nanomedicine (2013)

Fourier transform infrared spectra of (A) the neat PVA/COS nanofiber, (B) the PVA/COS/AgNO3 nanofiber, and (C) the PVA/COS-AgNP nanofiber.Abbreviations: AgNP, silver nanoparticle; COS, chitosan oligosaccharide; PVA, poly(vinyl alcohol).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3818021&req=5

f4-ijn-8-4131: Fourier transform infrared spectra of (A) the neat PVA/COS nanofiber, (B) the PVA/COS/AgNO3 nanofiber, and (C) the PVA/COS-AgNP nanofiber.Abbreviations: AgNP, silver nanoparticle; COS, chitosan oligosaccharide; PVA, poly(vinyl alcohol).
Mentions: The Fourier transform infrared spectra for the PVA/COS, PVA/COS/AgNO3, and PVA/COS-AgNP nanofibers are shown in Figure 4. The characteristic peaks of the neat PVA/COS nanofibers were assigned as follows: 3,308 cm−1 (N-H group), 2,942 cm−1 (CH2 asymmetric vibration), and 1,715 and 1,085 cm−1 (C=O bond). The peak at 1,248 cm−1 was associated with C=O=C vibrations and indicates the cross-linking of some PVA radicals.21 For the PVA/COS and PVA/COS/AgNO3 mats, the two spectra were similar with no shifts in any of the peaks, suggesting that the AgNPs were physically entrapped in the scaffold by van der Waals forces. Similar results have been reported by others.22,23 A significant change in the shape of the band at 3,338 cm−1 was observed between the PVA/COS nanofibers and the PVA/COS-AgNP nanofibers, indicating that N-H vibration was affected by attachment of the AgNPs.

Bottom Line: The AgNPs were reduced by COS prior to electrospinning or Ag(+) was reduced via ultraviolet irradiation in nanofibers.The nanofibers significantly inhibited growth of Escherichia coli and Staphylococcus aureus bacteria.The results of our in vitro and in vivo animal experiments suggest that PVA/COS-AgNP nanofibers should be of greater interest than PVA/COS/AgNO3 nanofibers for clinical use as a bioactive wound dressing.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, People's Republic of China.

ABSTRACT
In this study, a mixture of poly(vinyl alcohol) (PVA) and chitosan oligosaccharides (COS) was electrospun with silver nanoparticles (AgNPs) to produce fibrous mats for use in wound healing. The AgNPs were reduced by COS prior to electrospinning or Ag(+) was reduced via ultraviolet irradiation in nanofibers. The morphologies of the PVA/COS/AgNO3 and PVA/COS-AgNP nanofibers were analyzed by scanning electron microscopy. Formation of the AgNPs was investigated by field emission transmission electron microscopy, ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction. We also evaluated the biocompatibility of the nanofibers, particularly their cytotoxicity to human skin fibroblasts and potential to cause primary skin irritation. The in vitro antibacterial activity and in vivo wound healing capacity of the nanofibers were also investigated. The nanofibers had a smooth surface with an average diameter of 130-192 nm. The diameters of the AgNPs were in the range of 15-22 nm. The nanofibers significantly inhibited growth of Escherichia coli and Staphylococcus aureus bacteria. PVA/COS-AgNP nanofibers accelerated the rate of wound healing over that of the control (gauze). The results of our in vitro and in vivo animal experiments suggest that PVA/COS-AgNP nanofibers should be of greater interest than PVA/COS/AgNO3 nanofibers for clinical use as a bioactive wound dressing.

Show MeSH
Related in: MedlinePlus