Pleurotus ostreatus manganese-dependent peroxidase silencing impairs decolourization of Orange II.
Bottom Line: Relative real-time PCR quantification analysis confirmed that all the nine genes are transcribed, and that Mn(2+) amendment results in a drastic increase in the transcript levels of the predominantly expressed MnP genes (mnp 3 and mnp 9), while decreasing versatile peroxidase gene transcription (mnp 4).Knock-down of mnp 3 resulted in the reduction of fungal OII decolourization capacity, which was co-linear with marked silencing of the Mn(2+)-dependent peroxidase genes mnp 3 and mnp 9.This is the first direct genetic proof of an association between MnP gene expression levels and azo dye decolourization capacity in P. ostreatus, which may have significant implication on understanding the mechanisms governing lignin biodegradation.
Affiliation: Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel.Show MeSH
Related in: MedlinePlus
Mentions: In order to determine whether pTMS1 transformants were affected in their ability to decolourize OII, the different strains were grown on solid GP medium containing OII, amended with 27 µM of Mn2+, under carboxin selection pressure. Growth and decolourization, after 10 days of incubation, were monitored and compared with the untransformed wild‐type strain PC9 (Fig. 4A). TC3 exhibited similar growth (±3%) and decolourization (±5%) rates as PC9. pTMS1 transformants showed marked variability in their growth and OII decolourization capabilities. Of these, four strains, designated TS1, TS9, TS24 and TS30, which grew similarly to the wild‐type (88–94%), but showed high inhibition (90–99%) in their OII decolourization capacity in comparison to PC9 and TC3, were selected for further examination (Fig. 4B).
Affiliation: Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel.