Hyperthermostable acetyl xylan esterase.
Bottom Line: Thermotoga maritima AxeA represents the most thermostable acetyl xylan esterase known to date.Differential scanning calorimetry analysis of the thermal stability of AxeA corroborated its extreme heat resistance.A multi-phasic unfolding behaviour was found, with two apparent exothermic peaks at approximately 100-104 °C and 107.5 °C.
Affiliation: Institut f. Mikrobiologie und Genetik, Georg-August-Universität, Grisebachstr. 8, D-37077 Goettingen, Germany.Show MeSH
Related in: MedlinePlus
Mentions: Thermotoga maritima AxeA displayed maximum deacetylation activity at pH 6.5, and revealed more than 50% of its maximum activity between pH 5.0 and pH 7.5. The temperature at which the highest deacetylation activity in a 10 min assay was recorded was 90°C. A sharp drop in its relative activity was observed above 90°C (Fig. 3).
Affiliation: Institut f. Mikrobiologie und Genetik, Georg-August-Universität, Grisebachstr. 8, D-37077 Goettingen, Germany.