Limits...
Hyperthermostable acetyl xylan esterase.

Drzewiecki K, Angelov A, Ballschmiter M, Tiefenbach KJ, Sterner R, Liebl W - Microb Biotechnol (2009)

Bottom Line: Thermotoga maritima AxeA represents the most thermostable acetyl xylan esterase known to date.Differential scanning calorimetry analysis of the thermal stability of AxeA corroborated its extreme heat resistance.A multi-phasic unfolding behaviour was found, with two apparent exothermic peaks at approximately 100-104 °C and 107.5 °C.

View Article: PubMed Central - PubMed

Affiliation: Institut f. Mikrobiologie und Genetik, Georg-August-Universität, Grisebachstr. 8, D-37077 Goettingen, Germany.

Show MeSH

Related in: MedlinePlus

pH dependence (at 70°C) and temperature dependence (at pH 5.5) of AxeA activity, using a 10 min assay and chemically acetylated xylan as the substrate.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3815950&req=5

f3: pH dependence (at 70°C) and temperature dependence (at pH 5.5) of AxeA activity, using a 10 min assay and chemically acetylated xylan as the substrate.

Mentions: Thermotoga maritima AxeA displayed maximum deacetylation activity at pH 6.5, and revealed more than 50% of its maximum activity between pH 5.0 and pH 7.5. The temperature at which the highest deacetylation activity in a 10 min assay was recorded was 90°C. A sharp drop in its relative activity was observed above 90°C (Fig. 3).


Hyperthermostable acetyl xylan esterase.

Drzewiecki K, Angelov A, Ballschmiter M, Tiefenbach KJ, Sterner R, Liebl W - Microb Biotechnol (2009)

pH dependence (at 70°C) and temperature dependence (at pH 5.5) of AxeA activity, using a 10 min assay and chemically acetylated xylan as the substrate.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815950&req=5

f3: pH dependence (at 70°C) and temperature dependence (at pH 5.5) of AxeA activity, using a 10 min assay and chemically acetylated xylan as the substrate.
Mentions: Thermotoga maritima AxeA displayed maximum deacetylation activity at pH 6.5, and revealed more than 50% of its maximum activity between pH 5.0 and pH 7.5. The temperature at which the highest deacetylation activity in a 10 min assay was recorded was 90°C. A sharp drop in its relative activity was observed above 90°C (Fig. 3).

Bottom Line: Thermotoga maritima AxeA represents the most thermostable acetyl xylan esterase known to date.Differential scanning calorimetry analysis of the thermal stability of AxeA corroborated its extreme heat resistance.A multi-phasic unfolding behaviour was found, with two apparent exothermic peaks at approximately 100-104 °C and 107.5 °C.

View Article: PubMed Central - PubMed

Affiliation: Institut f. Mikrobiologie und Genetik, Georg-August-Universität, Grisebachstr. 8, D-37077 Goettingen, Germany.

Show MeSH
Related in: MedlinePlus