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Enantioselective kinetic resolution of phenylalkyl carboxylic acids using metagenome-derived esterases.

Fernández-Álvaro E, Kourist R, Winter J, Böttcher D, Liebeton K, Naumer C, Eck J, Leggewie C, Jaeger KE, Streit W, Bornscheuer UT - Microb Biotechnol (2009)

Bottom Line: Out of these, 20 enzymes were found to be active and were subjected to analytical scale biocatalysis in order to determine their enantioselectivity.The most enantioselective and also enantiocomplementary biocatalysts were then used for preparative scale reactions.Thus, both enantiomers of each of the three phenylalkyl carboxylic acids studied could be obtained in excellent optical purity and high yields.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Biotechnology and Enzyme Catalysis, Institute of Biochemistry, Greifswald University, Felix-Hausdorff-Str. 4, D-17487 Greifswald, Germany.

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Esterases showing the highest enantioselectivity and opposite enantiopreference in the kinetic resolutions of substrates 1b–3b.
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f2: Esterases showing the highest enantioselectivity and opposite enantiopreference in the kinetic resolutions of substrates 1b–3b.

Mentions: In the first step, a library of 83 esterases from metagenomic origin was screened for activity towards the ethyl esters 1b–3b using a microtiter plate‐based method (Baumann et al., 2000) with bromothymol blue serving as pH indicator. This led to the identification of 20 enzymes, which were then subjected to analytical scale kinetic resolutions for the determination of enantioselectivity and ‐preference by gas chromatographic analysis. The best hits are shown in Fig. 2. Next, the reaction conditions were further optimized by variation of reaction temperature (from 30°C to 20°C) and the addition of the cosolvent DMSO. Thus, at least two enzymes were found for each substrate exhibiting high enantioselectivity (E‐values > 50) and complementary enantiopreference (Table 1).


Enantioselective kinetic resolution of phenylalkyl carboxylic acids using metagenome-derived esterases.

Fernández-Álvaro E, Kourist R, Winter J, Böttcher D, Liebeton K, Naumer C, Eck J, Leggewie C, Jaeger KE, Streit W, Bornscheuer UT - Microb Biotechnol (2009)

Esterases showing the highest enantioselectivity and opposite enantiopreference in the kinetic resolutions of substrates 1b–3b.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815947&req=5

f2: Esterases showing the highest enantioselectivity and opposite enantiopreference in the kinetic resolutions of substrates 1b–3b.
Mentions: In the first step, a library of 83 esterases from metagenomic origin was screened for activity towards the ethyl esters 1b–3b using a microtiter plate‐based method (Baumann et al., 2000) with bromothymol blue serving as pH indicator. This led to the identification of 20 enzymes, which were then subjected to analytical scale kinetic resolutions for the determination of enantioselectivity and ‐preference by gas chromatographic analysis. The best hits are shown in Fig. 2. Next, the reaction conditions were further optimized by variation of reaction temperature (from 30°C to 20°C) and the addition of the cosolvent DMSO. Thus, at least two enzymes were found for each substrate exhibiting high enantioselectivity (E‐values > 50) and complementary enantiopreference (Table 1).

Bottom Line: Out of these, 20 enzymes were found to be active and were subjected to analytical scale biocatalysis in order to determine their enantioselectivity.The most enantioselective and also enantiocomplementary biocatalysts were then used for preparative scale reactions.Thus, both enantiomers of each of the three phenylalkyl carboxylic acids studied could be obtained in excellent optical purity and high yields.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Biotechnology and Enzyme Catalysis, Institute of Biochemistry, Greifswald University, Felix-Hausdorff-Str. 4, D-17487 Greifswald, Germany.

Show MeSH
Related in: MedlinePlus