Potential role of oxidative exoenzymes of the extremophilic fungus Pestalotiopsis palmarum BM-04 in biotransformation of extra-heavy crude oil.
Bottom Line: UV-visible spectrophotometry analysis revealed the oxidation of the petroporphyrins in the asphaltenes fraction of biotreated EHCO.Tolerance assays showed the ability of this fungus to grow up to 50,000 p.p.m. of EHCO and 2000 mM of NaCl.These results suggest that P. palmarum BM-04 is a hopeful alternative to be used in remediation processes in extreme environmental conditions of salinity and EHCO contamination, such as the drilling waste from the OOB.
Affiliation: Área de Energía y Ambiente, Instituto de Estudios Avanzados (IDEA), Sartenejas, Caracas, 1080, Venezuela.Show MeSH
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Mentions: In order to determine the potential of P. palmarum BM-04 to grow and tolerate extreme environmental conditions of salinity and EHCO contamination in hypothetical bioremediation processes, the fungus was grown in agar BSM medium with different concentrations of Carabobo-EHCO or NaCl as described in Experimental procedures. The DGC multiple comparison tests was used to detected significant differences among treatment and control. Figure 3A and B shows the range of growth in 0, 125, 250, 500, 1000 and 2000 mM of NaCl; and 1000, 5000, 10 000, 20 000 and 50 000 p.p.m. of EHCO respectively.
Affiliation: Área de Energía y Ambiente, Instituto de Estudios Avanzados (IDEA), Sartenejas, Caracas, 1080, Venezuela.