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Glucose metabolic flux distribution of Lactobacillus amylophilus during lactic acid production using kitchen waste saccharified solution.

Liu J, Wang Q, Zou H, Liu Y, Wang J, Gan K, Xiang J - Microb Biotechnol (2013)

Bottom Line: The flux to the EMP was also effectively increased.However, the addition of Zn(2+) showed an opposite effect, decreasing the lactic acid production.In conclusion it is feasible and effective means using GC-MS, isotope experiment and MATLAB software to integrate research the metabolic flux distribution of lactic acid bacteria, and the results provide the theoretical foundation for similar metabolic flux distribution.

View Article: PubMed Central - PubMed

Affiliation: School of Civil and Environmental Engineering, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing, 100083, China; Key Laboratory of Educational Ministry for High Efficient Mining and Safety in Metal Mine, University of Science and Technology Beijing, Beijing, 100083, China.

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Effect of Zn2+ on the activity of LDH, PDC, PC and PDHc. Dashed lines represent the control.
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fig05: Effect of Zn2+ on the activity of LDH, PDC, PC and PDHc. Dashed lines represent the control.

Mentions: In contrast to the system supplied with Fe3+, the LDH activity in the system supplied with Zn2+ was lower than the control, and the activity of PDC, PC and PDHc were higher (Fig. 5), which decreases the lactic acid conversion rate and increases the conversion rate of heteroacids and alcohols (Table 1). Therefore, the addition of Zn2+ to lactic acid fermentation system is unsuitable for L. amylophilus. However, this also suggests that Zn2+ significantly affects microbial activity and it could be considered as an inhibitor used in other similarly fermentation systems.


Glucose metabolic flux distribution of Lactobacillus amylophilus during lactic acid production using kitchen waste saccharified solution.

Liu J, Wang Q, Zou H, Liu Y, Wang J, Gan K, Xiang J - Microb Biotechnol (2013)

Effect of Zn2+ on the activity of LDH, PDC, PC and PDHc. Dashed lines represent the control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3815935&req=5

fig05: Effect of Zn2+ on the activity of LDH, PDC, PC and PDHc. Dashed lines represent the control.
Mentions: In contrast to the system supplied with Fe3+, the LDH activity in the system supplied with Zn2+ was lower than the control, and the activity of PDC, PC and PDHc were higher (Fig. 5), which decreases the lactic acid conversion rate and increases the conversion rate of heteroacids and alcohols (Table 1). Therefore, the addition of Zn2+ to lactic acid fermentation system is unsuitable for L. amylophilus. However, this also suggests that Zn2+ significantly affects microbial activity and it could be considered as an inhibitor used in other similarly fermentation systems.

Bottom Line: The flux to the EMP was also effectively increased.However, the addition of Zn(2+) showed an opposite effect, decreasing the lactic acid production.In conclusion it is feasible and effective means using GC-MS, isotope experiment and MATLAB software to integrate research the metabolic flux distribution of lactic acid bacteria, and the results provide the theoretical foundation for similar metabolic flux distribution.

View Article: PubMed Central - PubMed

Affiliation: School of Civil and Environmental Engineering, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing, 100083, China; Key Laboratory of Educational Ministry for High Efficient Mining and Safety in Metal Mine, University of Science and Technology Beijing, Beijing, 100083, China.

Show MeSH
Related in: MedlinePlus