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Glucose metabolic flux distribution of Lactobacillus amylophilus during lactic acid production using kitchen waste saccharified solution.

Liu J, Wang Q, Zou H, Liu Y, Wang J, Gan K, Xiang J - Microb Biotechnol (2013)

Bottom Line: The flux to the EMP was also effectively increased.However, the addition of Zn(2+) showed an opposite effect, decreasing the lactic acid production.In conclusion it is feasible and effective means using GC-MS, isotope experiment and MATLAB software to integrate research the metabolic flux distribution of lactic acid bacteria, and the results provide the theoretical foundation for similar metabolic flux distribution.

View Article: PubMed Central - PubMed

Affiliation: School of Civil and Environmental Engineering, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing, 100083, China; Key Laboratory of Educational Ministry for High Efficient Mining and Safety in Metal Mine, University of Science and Technology Beijing, Beijing, 100083, China.

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Effect of Fe3+ on the activity of LDH, PDC, PC and PDHc. Dashed lines represent the control.
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fig04: Effect of Fe3+ on the activity of LDH, PDC, PC and PDHc. Dashed lines represent the control.

Mentions: Figure 4 shows the changes in the activity of the four key enzymes relative to fermentation time. The LDH activity in the KWSS system supplied with Fe3+ was higher than that of the control (Fig. 4A). After 48 h of fermentation, the highest enzyme activity, 12.08 U mg−1, was obtained, which is 53.11% higher than the highest activity in the control. Moreover, the addition of Fe3+ effectively inhibited the activity of PDC, PC and PDHc. The activity of the three enzymes were all lower than in the control, which indicates that Fe3+ is an effective promoting factor of L. amylophilus lactic acid fermentation.


Glucose metabolic flux distribution of Lactobacillus amylophilus during lactic acid production using kitchen waste saccharified solution.

Liu J, Wang Q, Zou H, Liu Y, Wang J, Gan K, Xiang J - Microb Biotechnol (2013)

Effect of Fe3+ on the activity of LDH, PDC, PC and PDHc. Dashed lines represent the control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3815935&req=5

fig04: Effect of Fe3+ on the activity of LDH, PDC, PC and PDHc. Dashed lines represent the control.
Mentions: Figure 4 shows the changes in the activity of the four key enzymes relative to fermentation time. The LDH activity in the KWSS system supplied with Fe3+ was higher than that of the control (Fig. 4A). After 48 h of fermentation, the highest enzyme activity, 12.08 U mg−1, was obtained, which is 53.11% higher than the highest activity in the control. Moreover, the addition of Fe3+ effectively inhibited the activity of PDC, PC and PDHc. The activity of the three enzymes were all lower than in the control, which indicates that Fe3+ is an effective promoting factor of L. amylophilus lactic acid fermentation.

Bottom Line: The flux to the EMP was also effectively increased.However, the addition of Zn(2+) showed an opposite effect, decreasing the lactic acid production.In conclusion it is feasible and effective means using GC-MS, isotope experiment and MATLAB software to integrate research the metabolic flux distribution of lactic acid bacteria, and the results provide the theoretical foundation for similar metabolic flux distribution.

View Article: PubMed Central - PubMed

Affiliation: School of Civil and Environmental Engineering, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing, 100083, China; Key Laboratory of Educational Ministry for High Efficient Mining and Safety in Metal Mine, University of Science and Technology Beijing, Beijing, 100083, China.

Show MeSH
Related in: MedlinePlus