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Glucose metabolic flux distribution of Lactobacillus amylophilus during lactic acid production using kitchen waste saccharified solution.

Liu J, Wang Q, Zou H, Liu Y, Wang J, Gan K, Xiang J - Microb Biotechnol (2013)

Bottom Line: The flux to the EMP was also effectively increased.However, the addition of Zn(2+) showed an opposite effect, decreasing the lactic acid production.In conclusion it is feasible and effective means using GC-MS, isotope experiment and MATLAB software to integrate research the metabolic flux distribution of lactic acid bacteria, and the results provide the theoretical foundation for similar metabolic flux distribution.

View Article: PubMed Central - PubMed

Affiliation: School of Civil and Environmental Engineering, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing, 100083, China; Key Laboratory of Educational Ministry for High Efficient Mining and Safety in Metal Mine, University of Science and Technology Beijing, Beijing, 100083, China.

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Metabolic flux distribution of Lactobacillus amylophilus in 13C-labelled KWSS.
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fig02: Metabolic flux distribution of Lactobacillus amylophilus in 13C-labelled KWSS.

Mentions: The intracellular metabolic flux of L. amylophilus was obtained using the matrix in MATLAB based on the extracellular flow and amino acid data. As shown in Fig. 2, glucose was biodegraded by L. amylophilus through the pentose phosphate pathway (HMP) and the glycolytic pathway (EMP). In the KWSS, 36.7% of the metabolic flux entered the HMP pathway, whereas 60.6% entered the EMP pathway. The conversion rate of glucose to lactic acid through the EMP pathway was 86.6% (1.732 mol mol−1). The conversion rate with KWSS was slightly lower than that in MRS medium (1.85 mol mol−1 to 1.95 mol mol−1), but it was still greater than 80%; thus, the bacteria underwent homofermentation. These results indicate the feasibility of using L. amylophilus to produce lactic acid from KWSS.


Glucose metabolic flux distribution of Lactobacillus amylophilus during lactic acid production using kitchen waste saccharified solution.

Liu J, Wang Q, Zou H, Liu Y, Wang J, Gan K, Xiang J - Microb Biotechnol (2013)

Metabolic flux distribution of Lactobacillus amylophilus in 13C-labelled KWSS.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3815935&req=5

fig02: Metabolic flux distribution of Lactobacillus amylophilus in 13C-labelled KWSS.
Mentions: The intracellular metabolic flux of L. amylophilus was obtained using the matrix in MATLAB based on the extracellular flow and amino acid data. As shown in Fig. 2, glucose was biodegraded by L. amylophilus through the pentose phosphate pathway (HMP) and the glycolytic pathway (EMP). In the KWSS, 36.7% of the metabolic flux entered the HMP pathway, whereas 60.6% entered the EMP pathway. The conversion rate of glucose to lactic acid through the EMP pathway was 86.6% (1.732 mol mol−1). The conversion rate with KWSS was slightly lower than that in MRS medium (1.85 mol mol−1 to 1.95 mol mol−1), but it was still greater than 80%; thus, the bacteria underwent homofermentation. These results indicate the feasibility of using L. amylophilus to produce lactic acid from KWSS.

Bottom Line: The flux to the EMP was also effectively increased.However, the addition of Zn(2+) showed an opposite effect, decreasing the lactic acid production.In conclusion it is feasible and effective means using GC-MS, isotope experiment and MATLAB software to integrate research the metabolic flux distribution of lactic acid bacteria, and the results provide the theoretical foundation for similar metabolic flux distribution.

View Article: PubMed Central - PubMed

Affiliation: School of Civil and Environmental Engineering, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing, 100083, China; Key Laboratory of Educational Ministry for High Efficient Mining and Safety in Metal Mine, University of Science and Technology Beijing, Beijing, 100083, China.

Show MeSH
Related in: MedlinePlus