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Microbial production and chemical transformation of poly-γ-glutamate.

Ashiuchi M - Microb Biotechnol (2013)

Bottom Line: The occurrence of multiple carboxyl residues in PGA likely plays a role in its relative unsuitability for the development of bio-nylon plastics and thus, establishment of an efficient PGA-reforming strategy is of great importance.Aside from the potential applications of PGA proposed to date, a new technique for chemical transformation of PGA is also discussed.Finally, some techniques for PGA and its derivatives in advanced material technology are presented.

View Article: PubMed Central - PubMed

Affiliation: Agricultural Science, Graduate School of Integrated Arts and Sciences, Kochi University, Nankoku, Kochi, 783-8502, Japan.

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Proposed reaction mechanism for PGA production (Ashiuchi, 2010). Detailed explanation about the reactions steps is described in the Biosynthesis subsection ‘Reaction mechanism’.
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fig03: Proposed reaction mechanism for PGA production (Ashiuchi, 2010). Detailed explanation about the reactions steps is described in the Biosynthesis subsection ‘Reaction mechanism’.

Mentions: The mechanism for DL-PGA synthesis has been proposed (Fig. 3): step (A), the transfer of the phosphoryl group of ATP to the C-terminal carboxyl group of a growing chain and the release of the resulting ADP from the active site of the enzyme; step (B), the formation of an amide linkage via nucleophilic attack of the amino group of either a d- or l-glutamate on the phosphorylated carboxyl group; and step (C), the export of DL-PGA after multiple iterations of steps (A) and (B) within the enzyme. In principle, PGA is not covalently bound to a membrane-associated enzyme at any stage.


Microbial production and chemical transformation of poly-γ-glutamate.

Ashiuchi M - Microb Biotechnol (2013)

Proposed reaction mechanism for PGA production (Ashiuchi, 2010). Detailed explanation about the reactions steps is described in the Biosynthesis subsection ‘Reaction mechanism’.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3815933&req=5

fig03: Proposed reaction mechanism for PGA production (Ashiuchi, 2010). Detailed explanation about the reactions steps is described in the Biosynthesis subsection ‘Reaction mechanism’.
Mentions: The mechanism for DL-PGA synthesis has been proposed (Fig. 3): step (A), the transfer of the phosphoryl group of ATP to the C-terminal carboxyl group of a growing chain and the release of the resulting ADP from the active site of the enzyme; step (B), the formation of an amide linkage via nucleophilic attack of the amino group of either a d- or l-glutamate on the phosphorylated carboxyl group; and step (C), the export of DL-PGA after multiple iterations of steps (A) and (B) within the enzyme. In principle, PGA is not covalently bound to a membrane-associated enzyme at any stage.

Bottom Line: The occurrence of multiple carboxyl residues in PGA likely plays a role in its relative unsuitability for the development of bio-nylon plastics and thus, establishment of an efficient PGA-reforming strategy is of great importance.Aside from the potential applications of PGA proposed to date, a new technique for chemical transformation of PGA is also discussed.Finally, some techniques for PGA and its derivatives in advanced material technology are presented.

View Article: PubMed Central - PubMed

Affiliation: Agricultural Science, Graduate School of Integrated Arts and Sciences, Kochi University, Nankoku, Kochi, 783-8502, Japan.

Show MeSH
Related in: MedlinePlus