Microbial production and chemical transformation of poly-γ-glutamate.
Bottom Line: The occurrence of multiple carboxyl residues in PGA likely plays a role in its relative unsuitability for the development of bio-nylon plastics and thus, establishment of an efficient PGA-reforming strategy is of great importance.Aside from the potential applications of PGA proposed to date, a new technique for chemical transformation of PGA is also discussed.Finally, some techniques for PGA and its derivatives in advanced material technology are presented.
Affiliation: Agricultural Science, Graduate School of Integrated Arts and Sciences, Kochi University, Nankoku, Kochi, 783-8502, Japan.Show MeSH
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Mentions: The mechanism for DL-PGA synthesis has been proposed (Fig. 3): step (A), the transfer of the phosphoryl group of ATP to the C-terminal carboxyl group of a growing chain and the release of the resulting ADP from the active site of the enzyme; step (B), the formation of an amide linkage via nucleophilic attack of the amino group of either a d- or l-glutamate on the phosphorylated carboxyl group; and step (C), the export of DL-PGA after multiple iterations of steps (A) and (B) within the enzyme. In principle, PGA is not covalently bound to a membrane-associated enzyme at any stage.
Affiliation: Agricultural Science, Graduate School of Integrated Arts and Sciences, Kochi University, Nankoku, Kochi, 783-8502, Japan.