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The antagonistic strain Bacillus subtilis UMAF6639 also confers protection to melon plants against cucurbit powdery mildew by activation of jasmonate- and salicylic acid-dependent defence responses.

García-Gutiérrez L, Zeriouh H, Romero D, Cubero J, de Vicente A, Pérez-García A - Microb Biotechnol (2013)

Bottom Line: In a previous study, we found that UMAF6639 was able to induce systemic resistance (ISR) in melon and provide additional protection against powdery mildew.Our results demonstrated that UMAF6639 confers protection against cucurbit powdery mildew by activation of jasmonate- and salicylic acid-dependent defence responses, which include the production of reactive oxygen species and cell wall reinforcement.These results reinforce the biotechnological potential of UMAF6639 as a biological control agent.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Hortofruticultura Subtropical y Mediterránea 'La Mayora'-IHSM-UMA-CSIC, Departamento de Microbiología, Universidad de Málaga, Bulevar Louis Pasteur 31-Campus Universitario de Teatinos, 29071 Málaga, Spain.

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Histochemical analysis of the production reactive oxygen species in the leaves of melon plants bacterized with B. subtilis UMAF6639 and infected by powdery mildew. Melon plants were bacterized and inoculated with P. fusca as described in Experimental procedures. Detection of hydrogen peroxide (H2O2) was performed according to the DAB-uptake method, using bright light microscopy. Arrowheads indicate epidermal cells accumulating reddish-brown precipitates due to H2O2 production. Pictures were taken 72 h after inoculation of the fungal pathogen. Scale bars represent 50 μm (A) and 100 μm (rest of the plates).
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fig04: Histochemical analysis of the production reactive oxygen species in the leaves of melon plants bacterized with B. subtilis UMAF6639 and infected by powdery mildew. Melon plants were bacterized and inoculated with P. fusca as described in Experimental procedures. Detection of hydrogen peroxide (H2O2) was performed according to the DAB-uptake method, using bright light microscopy. Arrowheads indicate epidermal cells accumulating reddish-brown precipitates due to H2O2 production. Pictures were taken 72 h after inoculation of the fungal pathogen. Scale bars represent 50 μm (A) and 100 μm (rest of the plates).

Mentions: From previous findings it appeared that B. subtilis UMAF6639 triggers the SA and JA pathways which may be related to the ISR response. Thus, we asked which defence mechanisms of melon plants were involved in the ISR triggered after bacterization with UMAF6639 and exposition to P. fusca. The production of reactive oxygen species and the accumulation of cell wall deposits were histochemically examined in leaves of melon plants previously bacterized with B. subtilis UMAF6639 and subsequently inoculated with P. fusca. Representative pictures of the production of H2O2 by epidermal cells are shown in Fig. 4. The number of cells accumulating H2O2 was higher in bacterized than in untreated controls, 72 h after the inoculation of P. fusca. In addition, we observed that reacting cells appeared disperse in the leaves of untreated plants, while they clustered in the leaves of the UMAF6639-treated plants. This noticeable spatial distribution of reacting cells was interpreted as a response reaction at pathogen penetration sites.


The antagonistic strain Bacillus subtilis UMAF6639 also confers protection to melon plants against cucurbit powdery mildew by activation of jasmonate- and salicylic acid-dependent defence responses.

García-Gutiérrez L, Zeriouh H, Romero D, Cubero J, de Vicente A, Pérez-García A - Microb Biotechnol (2013)

Histochemical analysis of the production reactive oxygen species in the leaves of melon plants bacterized with B. subtilis UMAF6639 and infected by powdery mildew. Melon plants were bacterized and inoculated with P. fusca as described in Experimental procedures. Detection of hydrogen peroxide (H2O2) was performed according to the DAB-uptake method, using bright light microscopy. Arrowheads indicate epidermal cells accumulating reddish-brown precipitates due to H2O2 production. Pictures were taken 72 h after inoculation of the fungal pathogen. Scale bars represent 50 μm (A) and 100 μm (rest of the plates).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3815921&req=5

fig04: Histochemical analysis of the production reactive oxygen species in the leaves of melon plants bacterized with B. subtilis UMAF6639 and infected by powdery mildew. Melon plants were bacterized and inoculated with P. fusca as described in Experimental procedures. Detection of hydrogen peroxide (H2O2) was performed according to the DAB-uptake method, using bright light microscopy. Arrowheads indicate epidermal cells accumulating reddish-brown precipitates due to H2O2 production. Pictures were taken 72 h after inoculation of the fungal pathogen. Scale bars represent 50 μm (A) and 100 μm (rest of the plates).
Mentions: From previous findings it appeared that B. subtilis UMAF6639 triggers the SA and JA pathways which may be related to the ISR response. Thus, we asked which defence mechanisms of melon plants were involved in the ISR triggered after bacterization with UMAF6639 and exposition to P. fusca. The production of reactive oxygen species and the accumulation of cell wall deposits were histochemically examined in leaves of melon plants previously bacterized with B. subtilis UMAF6639 and subsequently inoculated with P. fusca. Representative pictures of the production of H2O2 by epidermal cells are shown in Fig. 4. The number of cells accumulating H2O2 was higher in bacterized than in untreated controls, 72 h after the inoculation of P. fusca. In addition, we observed that reacting cells appeared disperse in the leaves of untreated plants, while they clustered in the leaves of the UMAF6639-treated plants. This noticeable spatial distribution of reacting cells was interpreted as a response reaction at pathogen penetration sites.

Bottom Line: In a previous study, we found that UMAF6639 was able to induce systemic resistance (ISR) in melon and provide additional protection against powdery mildew.Our results demonstrated that UMAF6639 confers protection against cucurbit powdery mildew by activation of jasmonate- and salicylic acid-dependent defence responses, which include the production of reactive oxygen species and cell wall reinforcement.These results reinforce the biotechnological potential of UMAF6639 as a biological control agent.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Hortofruticultura Subtropical y Mediterránea 'La Mayora'-IHSM-UMA-CSIC, Departamento de Microbiología, Universidad de Málaga, Bulevar Louis Pasteur 31-Campus Universitario de Teatinos, 29071 Málaga, Spain.

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Related in: MedlinePlus