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The antagonistic strain Bacillus subtilis UMAF6639 also confers protection to melon plants against cucurbit powdery mildew by activation of jasmonate- and salicylic acid-dependent defence responses.

García-Gutiérrez L, Zeriouh H, Romero D, Cubero J, de Vicente A, Pérez-García A - Microb Biotechnol (2013)

Bottom Line: In a previous study, we found that UMAF6639 was able to induce systemic resistance (ISR) in melon and provide additional protection against powdery mildew.Our results demonstrated that UMAF6639 confers protection against cucurbit powdery mildew by activation of jasmonate- and salicylic acid-dependent defence responses, which include the production of reactive oxygen species and cell wall reinforcement.These results reinforce the biotechnological potential of UMAF6639 as a biological control agent.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Hortofruticultura Subtropical y Mediterránea 'La Mayora'-IHSM-UMA-CSIC, Departamento de Microbiología, Universidad de Málaga, Bulevar Louis Pasteur 31-Campus Universitario de Teatinos, 29071 Málaga, Spain.

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Suppressive effect of UMAF6639 towards cucurbit powdery mildew. A. Melon plants were bacterized and inoculated with P. fusca as described in Experimental procedures. Disease severity expressed as the percentage of the leaf surface covered by powdery mildew was recorded 18 days after pathogen challenge. Data represent the means of at least three independent experiments, and bars show the standard deviation. Treatments with the same letter are not significantly different at P = 0.05, according to Fisher's least-significant-difference test. A set of 15–20 plants was tested per treatment. B. Reduction of powdery mildew symptoms in melon seedlings by treatments with UMAF6639 following induction of a systemic resistance. Pictures were taken 18 days after inoculation with the fungal pathogen. Pictures: Untreated, leaf taken from an untreated plant, showing the upper surface completely covered by powdery mildew. UMAF6639, leaf taken from a plant treated with B. subtilis UMAF6639 showing significant reduction of powdery mildew symptoms.
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fig01: Suppressive effect of UMAF6639 towards cucurbit powdery mildew. A. Melon plants were bacterized and inoculated with P. fusca as described in Experimental procedures. Disease severity expressed as the percentage of the leaf surface covered by powdery mildew was recorded 18 days after pathogen challenge. Data represent the means of at least three independent experiments, and bars show the standard deviation. Treatments with the same letter are not significantly different at P = 0.05, according to Fisher's least-significant-difference test. A set of 15–20 plants was tested per treatment. B. Reduction of powdery mildew symptoms in melon seedlings by treatments with UMAF6639 following induction of a systemic resistance. Pictures were taken 18 days after inoculation with the fungal pathogen. Pictures: Untreated, leaf taken from an untreated plant, showing the upper surface completely covered by powdery mildew. UMAF6639, leaf taken from a plant treated with B. subtilis UMAF6639 showing significant reduction of powdery mildew symptoms.

Mentions: Despite its phyllospheric origin, UMAF6639 also provides protection against powdery mildew by means of ISR (Romero et al., 2004; García-Gutiérrez et al., 2012). The application of UMAF6639 to the roots of melon seedlings provoked a significant reduction in the disease severity (about 50%) 18 days after inoculation with the fungal pathogen, which was very similar to the protection provided by the rhizospheric strains Pseudomonas fluorescens UMAF6031 and Bacillus cereus UMAF8564 (Fig. 1A). This protective effect is illustrated in Fig. 1B, which shows the reduction of symptoms in a leaf of an UMAF6639-treated plant compared with an untreated control.


The antagonistic strain Bacillus subtilis UMAF6639 also confers protection to melon plants against cucurbit powdery mildew by activation of jasmonate- and salicylic acid-dependent defence responses.

García-Gutiérrez L, Zeriouh H, Romero D, Cubero J, de Vicente A, Pérez-García A - Microb Biotechnol (2013)

Suppressive effect of UMAF6639 towards cucurbit powdery mildew. A. Melon plants were bacterized and inoculated with P. fusca as described in Experimental procedures. Disease severity expressed as the percentage of the leaf surface covered by powdery mildew was recorded 18 days after pathogen challenge. Data represent the means of at least three independent experiments, and bars show the standard deviation. Treatments with the same letter are not significantly different at P = 0.05, according to Fisher's least-significant-difference test. A set of 15–20 plants was tested per treatment. B. Reduction of powdery mildew symptoms in melon seedlings by treatments with UMAF6639 following induction of a systemic resistance. Pictures were taken 18 days after inoculation with the fungal pathogen. Pictures: Untreated, leaf taken from an untreated plant, showing the upper surface completely covered by powdery mildew. UMAF6639, leaf taken from a plant treated with B. subtilis UMAF6639 showing significant reduction of powdery mildew symptoms.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3815921&req=5

fig01: Suppressive effect of UMAF6639 towards cucurbit powdery mildew. A. Melon plants were bacterized and inoculated with P. fusca as described in Experimental procedures. Disease severity expressed as the percentage of the leaf surface covered by powdery mildew was recorded 18 days after pathogen challenge. Data represent the means of at least three independent experiments, and bars show the standard deviation. Treatments with the same letter are not significantly different at P = 0.05, according to Fisher's least-significant-difference test. A set of 15–20 plants was tested per treatment. B. Reduction of powdery mildew symptoms in melon seedlings by treatments with UMAF6639 following induction of a systemic resistance. Pictures were taken 18 days after inoculation with the fungal pathogen. Pictures: Untreated, leaf taken from an untreated plant, showing the upper surface completely covered by powdery mildew. UMAF6639, leaf taken from a plant treated with B. subtilis UMAF6639 showing significant reduction of powdery mildew symptoms.
Mentions: Despite its phyllospheric origin, UMAF6639 also provides protection against powdery mildew by means of ISR (Romero et al., 2004; García-Gutiérrez et al., 2012). The application of UMAF6639 to the roots of melon seedlings provoked a significant reduction in the disease severity (about 50%) 18 days after inoculation with the fungal pathogen, which was very similar to the protection provided by the rhizospheric strains Pseudomonas fluorescens UMAF6031 and Bacillus cereus UMAF8564 (Fig. 1A). This protective effect is illustrated in Fig. 1B, which shows the reduction of symptoms in a leaf of an UMAF6639-treated plant compared with an untreated control.

Bottom Line: In a previous study, we found that UMAF6639 was able to induce systemic resistance (ISR) in melon and provide additional protection against powdery mildew.Our results demonstrated that UMAF6639 confers protection against cucurbit powdery mildew by activation of jasmonate- and salicylic acid-dependent defence responses, which include the production of reactive oxygen species and cell wall reinforcement.These results reinforce the biotechnological potential of UMAF6639 as a biological control agent.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Hortofruticultura Subtropical y Mediterránea 'La Mayora'-IHSM-UMA-CSIC, Departamento de Microbiología, Universidad de Málaga, Bulevar Louis Pasteur 31-Campus Universitario de Teatinos, 29071 Málaga, Spain.

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Related in: MedlinePlus