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Xenomic networks variability and adaptation traits in wood decaying fungi.

Morel M, Meux E, Mathieu Y, Thuillier A, Chibani K, Harvengt L, Jacquot JP, Gelhaye E - Microb Biotechnol (2013)

Bottom Line: Taking advantage of the recent release of numerous genomes of basidiomycetes, we show here that these multigenic families are extended and functionally related in wood-decaying fungi.Furthermore, we postulate that these rapidly evolving multigenic families could reflect the adaptation of these fungi to the diversity of their substrate and provide keys to understand their ecology.This is of particular importance for white biotechnology, this xenome being a putative target for improving degradation properties of these fungi in biomass valorization purposes.

View Article: PubMed Central - PubMed

Affiliation: Université de Lorraine, IAM, UMR 1136, IFR 110 EFABA, Vandoeuvre-lès-Nancy, F-54506, France. mmorel@scbiol.uhp-nancy.fr

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Heat maps for selected P. carnosa transcripts coding for CytP450 (A) and GSTs (B), during growth on YMPG (Y) or medium containing wood from fir (F), pine (P), spruce (S) or maple (M), as determined by mRNA-Seq. The colour scale represents the reads per kilobase of gene model per million mapped reads. The figure has been adapted from MacDonald and colleagues (2011).
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fig05: Heat maps for selected P. carnosa transcripts coding for CytP450 (A) and GSTs (B), during growth on YMPG (Y) or medium containing wood from fir (F), pine (P), spruce (S) or maple (M), as determined by mRNA-Seq. The colour scale represents the reads per kilobase of gene model per million mapped reads. The figure has been adapted from MacDonald and colleagues (2011).

Mentions: The direct correlation between the P450ome and GSTome was also investigated using the growing number of transcriptional studies. For example, specific CytP450-encoding genes found in P. chrysosporium are induced in presence of polycyclic aromatic hydrocarbons (Syed et al., 2010) and it is also the case for two Ure2p isoforms (Thuillier et al., 2011). In the same fungus, nonylphenol induces strongly three CytP450 and a Ure2p-encoding genes (Subramanian and Yadav, 2009). In the context of wood degradation, induction of different CytP450s-encoding genes has been observed in P. chrysosporium and P. placenta in a wood species-dependent manner (Vanden Wymelenberg et al., 2011) and P. carnosa (MacDonald et al., 2011). In P. carnosa, heat maps revealed variable expression of CytP450 and GST genes during growth on fir, pine, spruce or maple (Fig. 5 adapted from MacDonald et al., 2011). Some transcripts have been found to be abundant in all wood degrading conditions, while others seem to accumulate in specific ones. As an example CytP450 (Gene ID 516) and GST (Gene ID 1262) exhibit the same transcript profile, i.e. an induction by all wood substrates. Similarly, CytP450 (Gene ID 3626) and GST (Gene ID 2315) are both induced specifically by fir (Fig. 5). Concomitant analysis of CytP450 and GST expression could be a starting point to establish a functional link between isoforms.


Xenomic networks variability and adaptation traits in wood decaying fungi.

Morel M, Meux E, Mathieu Y, Thuillier A, Chibani K, Harvengt L, Jacquot JP, Gelhaye E - Microb Biotechnol (2013)

Heat maps for selected P. carnosa transcripts coding for CytP450 (A) and GSTs (B), during growth on YMPG (Y) or medium containing wood from fir (F), pine (P), spruce (S) or maple (M), as determined by mRNA-Seq. The colour scale represents the reads per kilobase of gene model per million mapped reads. The figure has been adapted from MacDonald and colleagues (2011).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3815920&req=5

fig05: Heat maps for selected P. carnosa transcripts coding for CytP450 (A) and GSTs (B), during growth on YMPG (Y) or medium containing wood from fir (F), pine (P), spruce (S) or maple (M), as determined by mRNA-Seq. The colour scale represents the reads per kilobase of gene model per million mapped reads. The figure has been adapted from MacDonald and colleagues (2011).
Mentions: The direct correlation between the P450ome and GSTome was also investigated using the growing number of transcriptional studies. For example, specific CytP450-encoding genes found in P. chrysosporium are induced in presence of polycyclic aromatic hydrocarbons (Syed et al., 2010) and it is also the case for two Ure2p isoforms (Thuillier et al., 2011). In the same fungus, nonylphenol induces strongly three CytP450 and a Ure2p-encoding genes (Subramanian and Yadav, 2009). In the context of wood degradation, induction of different CytP450s-encoding genes has been observed in P. chrysosporium and P. placenta in a wood species-dependent manner (Vanden Wymelenberg et al., 2011) and P. carnosa (MacDonald et al., 2011). In P. carnosa, heat maps revealed variable expression of CytP450 and GST genes during growth on fir, pine, spruce or maple (Fig. 5 adapted from MacDonald et al., 2011). Some transcripts have been found to be abundant in all wood degrading conditions, while others seem to accumulate in specific ones. As an example CytP450 (Gene ID 516) and GST (Gene ID 1262) exhibit the same transcript profile, i.e. an induction by all wood substrates. Similarly, CytP450 (Gene ID 3626) and GST (Gene ID 2315) are both induced specifically by fir (Fig. 5). Concomitant analysis of CytP450 and GST expression could be a starting point to establish a functional link between isoforms.

Bottom Line: Taking advantage of the recent release of numerous genomes of basidiomycetes, we show here that these multigenic families are extended and functionally related in wood-decaying fungi.Furthermore, we postulate that these rapidly evolving multigenic families could reflect the adaptation of these fungi to the diversity of their substrate and provide keys to understand their ecology.This is of particular importance for white biotechnology, this xenome being a putative target for improving degradation properties of these fungi in biomass valorization purposes.

View Article: PubMed Central - PubMed

Affiliation: Université de Lorraine, IAM, UMR 1136, IFR 110 EFABA, Vandoeuvre-lès-Nancy, F-54506, France. mmorel@scbiol.uhp-nancy.fr

Show MeSH
Related in: MedlinePlus