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The relationship between lignin peroxidase and manganese peroxidase production capacities and cultivation periods of mushrooms.

Xu JZ, Zhang JL, Hu KH, Zhang WG - Microb Biotechnol (2012)

Bottom Line: The results show that there are close relationships between LiP- and/or MnP-secretory capacities and the cultivation periods of mushrooms.This study provides the first evidence on the imitate relation between the level of secreted LiP and MnP activities and cultivation periods of mushrooms with non-laccase activity.Our study has significantly increased the understanding of the role of LiP and MnP in the growth and development of mushrooms with non-laccase activity.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences, Fujian Agriculture and Forestry University, FuZhou 350002, China.

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The qualitative assay of LiP and MnP after 7 days incubated in MYPGB at 24°C and pH 7.0 with shaking (100 r min−1). (A) Methylene blue qualitative assay of LiP from G. frondosa; (A′) methyl catechol qualitative assay of MnP from G. frondosa; (B) methylene blue qualitative assay of LiP from Agrocybe sp.; (B′) methyl catechol qualitative assay of MnP from Agrocybe sp. The left test tubes were control tests.
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fig01: The qualitative assay of LiP and MnP after 7 days incubated in MYPGB at 24°C and pH 7.0 with shaking (100 r min−1). (A) Methylene blue qualitative assay of LiP from G. frondosa; (A′) methyl catechol qualitative assay of MnP from G. frondosa; (B) methylene blue qualitative assay of LiP from Agrocybe sp.; (B′) methyl catechol qualitative assay of MnP from Agrocybe sp. The left test tubes were control tests.

Mentions: Peroxidase production capacities of different mushroom strains were compared and analysed by the colour change of mixture. The methylene blue and methyl catechol were used for a visual inspection for the LiP and MnP presence in the mixture respectively. The results are presented in Table 1 and Fig. 1. Ten different mushroom strains were divided into three groups based on peroxidase-secreting capacity. The colour change immediately shows high level of LiP or MnP production capacity. These strains including Stropharia rugoso, Hypsizigus marmoreus, Tricholoma lobyense, Agrocybe cylindracea, Agrocybe sp. and Fistulina hepatica could secrete LiP and MnP, which belonged to group I. Pleurotus nebrodeusis and Grifola frondosa could only secrete MnP, which belonged to group II. The other strains including Pholiota nameko and Lepista irina did not change the mixture colour after 1 h. Therefore, LiP and MnP were not detected in these strains, which belonged to group III. The strains in group I and group II also showed different levels of enzyme activities. The cultivation period from inoculations to the first time harvesting in solid-state systems showed great difference among 10 strains. The cultivation periods of those strains in group I were shown generally shorter than the other groups. Group II had middle level of cultivation periods, and group III had the longest cultivation periods among all groups. The strains in group I, group II or group III also showed different cultivation periods (Pang et al., 2003; Zhang, 2003; Sun et al., 2011). The results showed that LiP-secreting and MnP-secreting capacity and variety of mushroom are closely related to cultivation period. The mushroom strains have short cultivation periods because they can secrete LiP and MnP, and have high levels of enzyme activity. However, those other strains require long growing cycles due to the unitary enzyme and the low levels of enzyme activity, even no detectable enzyme activity. The results also indicated that the methylene blue reaction and the methyl catechol reaction can be used as rapid assay for visual inspection for the enzyme presence and capacity in the culture supernatant.


The relationship between lignin peroxidase and manganese peroxidase production capacities and cultivation periods of mushrooms.

Xu JZ, Zhang JL, Hu KH, Zhang WG - Microb Biotechnol (2012)

The qualitative assay of LiP and MnP after 7 days incubated in MYPGB at 24°C and pH 7.0 with shaking (100 r min−1). (A) Methylene blue qualitative assay of LiP from G. frondosa; (A′) methyl catechol qualitative assay of MnP from G. frondosa; (B) methylene blue qualitative assay of LiP from Agrocybe sp.; (B′) methyl catechol qualitative assay of MnP from Agrocybe sp. The left test tubes were control tests.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3815919&req=5

fig01: The qualitative assay of LiP and MnP after 7 days incubated in MYPGB at 24°C and pH 7.0 with shaking (100 r min−1). (A) Methylene blue qualitative assay of LiP from G. frondosa; (A′) methyl catechol qualitative assay of MnP from G. frondosa; (B) methylene blue qualitative assay of LiP from Agrocybe sp.; (B′) methyl catechol qualitative assay of MnP from Agrocybe sp. The left test tubes were control tests.
Mentions: Peroxidase production capacities of different mushroom strains were compared and analysed by the colour change of mixture. The methylene blue and methyl catechol were used for a visual inspection for the LiP and MnP presence in the mixture respectively. The results are presented in Table 1 and Fig. 1. Ten different mushroom strains were divided into three groups based on peroxidase-secreting capacity. The colour change immediately shows high level of LiP or MnP production capacity. These strains including Stropharia rugoso, Hypsizigus marmoreus, Tricholoma lobyense, Agrocybe cylindracea, Agrocybe sp. and Fistulina hepatica could secrete LiP and MnP, which belonged to group I. Pleurotus nebrodeusis and Grifola frondosa could only secrete MnP, which belonged to group II. The other strains including Pholiota nameko and Lepista irina did not change the mixture colour after 1 h. Therefore, LiP and MnP were not detected in these strains, which belonged to group III. The strains in group I and group II also showed different levels of enzyme activities. The cultivation period from inoculations to the first time harvesting in solid-state systems showed great difference among 10 strains. The cultivation periods of those strains in group I were shown generally shorter than the other groups. Group II had middle level of cultivation periods, and group III had the longest cultivation periods among all groups. The strains in group I, group II or group III also showed different cultivation periods (Pang et al., 2003; Zhang, 2003; Sun et al., 2011). The results showed that LiP-secreting and MnP-secreting capacity and variety of mushroom are closely related to cultivation period. The mushroom strains have short cultivation periods because they can secrete LiP and MnP, and have high levels of enzyme activity. However, those other strains require long growing cycles due to the unitary enzyme and the low levels of enzyme activity, even no detectable enzyme activity. The results also indicated that the methylene blue reaction and the methyl catechol reaction can be used as rapid assay for visual inspection for the enzyme presence and capacity in the culture supernatant.

Bottom Line: The results show that there are close relationships between LiP- and/or MnP-secretory capacities and the cultivation periods of mushrooms.This study provides the first evidence on the imitate relation between the level of secreted LiP and MnP activities and cultivation periods of mushrooms with non-laccase activity.Our study has significantly increased the understanding of the role of LiP and MnP in the growth and development of mushrooms with non-laccase activity.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences, Fujian Agriculture and Forestry University, FuZhou 350002, China.

Show MeSH