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Translocation and insecticidal activity of Bacillus thuringiensis living inside of plants.

Monnerat RG, Soares CM, Capdeville G, Jones G, Martins ES, Praça L, Cordeiro BA, Braz SV, dos Santos RC, Berry C - Microb Biotechnol (2009)

Bottom Line: The ability of B. thuringiensis to colonize plants as an endophyte was further established by the introduction of a strain marked by production of green fluorescent protein (GFP).Leaves taken from the treated plants were able to cause toxicity when fed to the Lepidoptera Spodoptera frugiperda (cotton) and Plutella xylostella (cabbage).These results open up new horizons for understanding the natural ecology and evolution of B. thuringiensis and use of B. thuringiensis in insect control.

View Article: PubMed Central - PubMed

Affiliation: Embrapa Recursos Geneticos e Biotecnologia, Parque Estação Biológica, Av W/5 Norte (final), CEP 70 770-900, Caixa Postal 02372, Brasília DF, Brazil. rose@cenargen.embrapa.br

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Confocal microscopy images of S. frugiperda larvae. Larvae were fed on leaves of cabbage plants inoculated with sterile NYSM medium (controls: A–D) or with Btk::GFP for 24 h (I–L) and larvae fed on diet containing 1.3 × 103 cells to Btk::GFP for 24 h. (E–H) Images show: fluorescence due to DAPI blue (A, E, I). Fluorescence at 488 nm due to GFP protein (C, G, K). Fluorescence at 594 nm due to anti‐GFP antibody (B, F, J); merged images of DAPI blue, GFP fluorescence and antibody fluorescence (D, H, L). The following structures are labelled: av, apical vesicles; bl, basal lamina; n, nucleus; sb, striated border.
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f5: Confocal microscopy images of S. frugiperda larvae. Larvae were fed on leaves of cabbage plants inoculated with sterile NYSM medium (controls: A–D) or with Btk::GFP for 24 h (I–L) and larvae fed on diet containing 1.3 × 103 cells to Btk::GFP for 24 h. (E–H) Images show: fluorescence due to DAPI blue (A, E, I). Fluorescence at 488 nm due to GFP protein (C, G, K). Fluorescence at 594 nm due to anti‐GFP antibody (B, F, J); merged images of DAPI blue, GFP fluorescence and antibody fluorescence (D, H, L). The following structures are labelled: av, apical vesicles; bl, basal lamina; n, nucleus; sb, striated border.

Mentions: To analyse the effects on insects in detail, the midguts of S. frugiperda fed for 24 h on leaves from Bt‐inoculated cotton plants and in artificial diet were dissected out and analysed using anti‐GFP antibodies. We found most bacteria in the insect midgut (Fig. 5). Other organs like adipose and tracheal systems were infected as well (not shown). Tissues from insects fed on control plants showed structural integrity and no fluorescence signal (Fig. 5).


Translocation and insecticidal activity of Bacillus thuringiensis living inside of plants.

Monnerat RG, Soares CM, Capdeville G, Jones G, Martins ES, Praça L, Cordeiro BA, Braz SV, dos Santos RC, Berry C - Microb Biotechnol (2009)

Confocal microscopy images of S. frugiperda larvae. Larvae were fed on leaves of cabbage plants inoculated with sterile NYSM medium (controls: A–D) or with Btk::GFP for 24 h (I–L) and larvae fed on diet containing 1.3 × 103 cells to Btk::GFP for 24 h. (E–H) Images show: fluorescence due to DAPI blue (A, E, I). Fluorescence at 488 nm due to GFP protein (C, G, K). Fluorescence at 594 nm due to anti‐GFP antibody (B, F, J); merged images of DAPI blue, GFP fluorescence and antibody fluorescence (D, H, L). The following structures are labelled: av, apical vesicles; bl, basal lamina; n, nucleus; sb, striated border.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815911&req=5

f5: Confocal microscopy images of S. frugiperda larvae. Larvae were fed on leaves of cabbage plants inoculated with sterile NYSM medium (controls: A–D) or with Btk::GFP for 24 h (I–L) and larvae fed on diet containing 1.3 × 103 cells to Btk::GFP for 24 h. (E–H) Images show: fluorescence due to DAPI blue (A, E, I). Fluorescence at 488 nm due to GFP protein (C, G, K). Fluorescence at 594 nm due to anti‐GFP antibody (B, F, J); merged images of DAPI blue, GFP fluorescence and antibody fluorescence (D, H, L). The following structures are labelled: av, apical vesicles; bl, basal lamina; n, nucleus; sb, striated border.
Mentions: To analyse the effects on insects in detail, the midguts of S. frugiperda fed for 24 h on leaves from Bt‐inoculated cotton plants and in artificial diet were dissected out and analysed using anti‐GFP antibodies. We found most bacteria in the insect midgut (Fig. 5). Other organs like adipose and tracheal systems were infected as well (not shown). Tissues from insects fed on control plants showed structural integrity and no fluorescence signal (Fig. 5).

Bottom Line: The ability of B. thuringiensis to colonize plants as an endophyte was further established by the introduction of a strain marked by production of green fluorescent protein (GFP).Leaves taken from the treated plants were able to cause toxicity when fed to the Lepidoptera Spodoptera frugiperda (cotton) and Plutella xylostella (cabbage).These results open up new horizons for understanding the natural ecology and evolution of B. thuringiensis and use of B. thuringiensis in insect control.

View Article: PubMed Central - PubMed

Affiliation: Embrapa Recursos Geneticos e Biotecnologia, Parque Estação Biológica, Av W/5 Norte (final), CEP 70 770-900, Caixa Postal 02372, Brasília DF, Brazil. rose@cenargen.embrapa.br

Show MeSH
Related in: MedlinePlus