Translocation and insecticidal activity of Bacillus thuringiensis living inside of plants.
Bottom Line: The ability of B. thuringiensis to colonize plants as an endophyte was further established by the introduction of a strain marked by production of green fluorescent protein (GFP).Leaves taken from the treated plants were able to cause toxicity when fed to the Lepidoptera Spodoptera frugiperda (cotton) and Plutella xylostella (cabbage).These results open up new horizons for understanding the natural ecology and evolution of B. thuringiensis and use of B. thuringiensis in insect control.
Affiliation: Embrapa Recursos Geneticos e Biotecnologia, Parque Estação Biológica, Av W/5 Norte (final), CEP 70 770-900, Caixa Postal 02372, Brasília DF, Brazil. email@example.comShow MeSH
Related in: MedlinePlus
Mentions: To analyse the effects on insects in detail, the midguts of S. frugiperda fed for 24 h on leaves from Bt‐inoculated cotton plants and in artificial diet were dissected out and analysed using anti‐GFP antibodies. We found most bacteria in the insect midgut (Fig. 5). Other organs like adipose and tracheal systems were infected as well (not shown). Tissues from insects fed on control plants showed structural integrity and no fluorescence signal (Fig. 5).
Affiliation: Embrapa Recursos Geneticos e Biotecnologia, Parque Estação Biológica, Av W/5 Norte (final), CEP 70 770-900, Caixa Postal 02372, Brasília DF, Brazil. firstname.lastname@example.org