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Translocation and insecticidal activity of Bacillus thuringiensis living inside of plants.

Monnerat RG, Soares CM, Capdeville G, Jones G, Martins ES, Praça L, Cordeiro BA, Braz SV, dos Santos RC, Berry C - Microb Biotechnol (2009)

Bottom Line: The ability of B. thuringiensis to colonize plants as an endophyte was further established by the introduction of a strain marked by production of green fluorescent protein (GFP).Leaves taken from the treated plants were able to cause toxicity when fed to the Lepidoptera Spodoptera frugiperda (cotton) and Plutella xylostella (cabbage).These results open up new horizons for understanding the natural ecology and evolution of B. thuringiensis and use of B. thuringiensis in insect control.

View Article: PubMed Central - PubMed

Affiliation: Embrapa Recursos Geneticos e Biotecnologia, Parque Estação Biológica, Av W/5 Norte (final), CEP 70 770-900, Caixa Postal 02372, Brasília DF, Brazil. rose@cenargen.embrapa.br

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Confocal microscopy images of the Btk::GFP, showing (A) control bacteria, (B) Btk::GFP labelled with Alexa 594 in the bacteria.
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f3: Confocal microscopy images of the Btk::GFP, showing (A) control bacteria, (B) Btk::GFP labelled with Alexa 594 in the bacteria.

Mentions: To get a better idea of the distribution of the bacterium, we looked at the presence of GFP in plants using an anti‐GFP antibody and laser confocal microscopy. The ability of the method to label GFP within non‐fixed bacteria was first confirmed using bacteria alone (Fig. 3). Analysis of control plants inoculated with non‐labelled bacteria showed only parenchyma (the cellular surface of the leaves) and guard‐cell autofluorescence in the tissue and no fluorescence signal in the veins (Fig. 4A–I). In contrast, observation of the leaves of inoculated plants showed a major presence of Btk::GFP in the veins, probably attached to the walls and almost no fluorescent signal in the parenchyma (Fig. 4). However, some labelling could sometimes be found in this tissue adjacent to the end of the veins (Fig. 4), indicating that the bacteria can reach the parenchyma in these specific regions.


Translocation and insecticidal activity of Bacillus thuringiensis living inside of plants.

Monnerat RG, Soares CM, Capdeville G, Jones G, Martins ES, Praça L, Cordeiro BA, Braz SV, dos Santos RC, Berry C - Microb Biotechnol (2009)

Confocal microscopy images of the Btk::GFP, showing (A) control bacteria, (B) Btk::GFP labelled with Alexa 594 in the bacteria.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815911&req=5

f3: Confocal microscopy images of the Btk::GFP, showing (A) control bacteria, (B) Btk::GFP labelled with Alexa 594 in the bacteria.
Mentions: To get a better idea of the distribution of the bacterium, we looked at the presence of GFP in plants using an anti‐GFP antibody and laser confocal microscopy. The ability of the method to label GFP within non‐fixed bacteria was first confirmed using bacteria alone (Fig. 3). Analysis of control plants inoculated with non‐labelled bacteria showed only parenchyma (the cellular surface of the leaves) and guard‐cell autofluorescence in the tissue and no fluorescence signal in the veins (Fig. 4A–I). In contrast, observation of the leaves of inoculated plants showed a major presence of Btk::GFP in the veins, probably attached to the walls and almost no fluorescent signal in the parenchyma (Fig. 4). However, some labelling could sometimes be found in this tissue adjacent to the end of the veins (Fig. 4), indicating that the bacteria can reach the parenchyma in these specific regions.

Bottom Line: The ability of B. thuringiensis to colonize plants as an endophyte was further established by the introduction of a strain marked by production of green fluorescent protein (GFP).Leaves taken from the treated plants were able to cause toxicity when fed to the Lepidoptera Spodoptera frugiperda (cotton) and Plutella xylostella (cabbage).These results open up new horizons for understanding the natural ecology and evolution of B. thuringiensis and use of B. thuringiensis in insect control.

View Article: PubMed Central - PubMed

Affiliation: Embrapa Recursos Geneticos e Biotecnologia, Parque Estação Biológica, Av W/5 Norte (final), CEP 70 770-900, Caixa Postal 02372, Brasília DF, Brazil. rose@cenargen.embrapa.br

Show MeSH
Related in: MedlinePlus