Translocation and insecticidal activity of Bacillus thuringiensis living inside of plants.
Bottom Line: The ability of B. thuringiensis to colonize plants as an endophyte was further established by the introduction of a strain marked by production of green fluorescent protein (GFP).Leaves taken from the treated plants were able to cause toxicity when fed to the Lepidoptera Spodoptera frugiperda (cotton) and Plutella xylostella (cabbage).These results open up new horizons for understanding the natural ecology and evolution of B. thuringiensis and use of B. thuringiensis in insect control.
Affiliation: Embrapa Recursos Geneticos e Biotecnologia, Parque Estação Biológica, Av W/5 Norte (final), CEP 70 770-900, Caixa Postal 02372, Brasília DF, Brazil. email@example.comShow MeSH
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Mentions: To confirm uptake of Bt into plants independent of GFP, accumulation of Bt in the leaves was studied by autoradiography following inoculation of plants with radiolabelled bacteria (Fig. 2). The radioactivity could be detected in all parts of inoculated plants. In contrast, the radioactivity was not detected in plants inoculated with the final wash solution used to remove residual, unincorporated 35S‐methionine from the preparation and thus providing a control demonstrating that the label observed on addition of Bt was not due to unincorporated radiolabel.
Affiliation: Embrapa Recursos Geneticos e Biotecnologia, Parque Estação Biológica, Av W/5 Norte (final), CEP 70 770-900, Caixa Postal 02372, Brasília DF, Brazil. firstname.lastname@example.org