Agrobacterium tumefaciens and A. rhizogenes use different proteins to transport bacterial DNA into the plant cell nucleus.
Bottom Line: Unlike VirE2, GALLS-FL contains ATP-binding and helicase motifs similar to those in TraA, a strand transferase involved in conjugation.GALLS-FL accumulates inside the nucleus where its predicted ATP-dependent strand transferase may pull T-strands into the nucleus.These different mechanisms for nuclear import of T-strands may affect the efficiency and quality of transgenic events in plant biotechnology applications.
Affiliation: Department of Microbiology, Oregon State University, Corvallis, OR 97331, USA. email@example.comShow MeSH
Mentions: Full‐length GALLS (GALLS‐FL) (Hodges et al., 2006) and VirE2 (Vergunst et al., 2000; 2003; Simone et al., 2001) contain C‐terminal signals for translocation into plant cells mediated by the VirB/D4 type IV secretion system (Fig. 1). VirE2 contains two NLSs (Citovsky et al., 1992; Zupan et al., 1996), whereas GALLS‐FL contains a single bipartite NLS (Fig. 1) (Hodges et al., 2004), which is important for its ability to substitute for VirE2 (Hodges et al., 2006). This indicates that GALLS‐FL performs a critical function inside the nucleus or at the nuclear membrane, as does VirE2.
Affiliation: Department of Microbiology, Oregon State University, Corvallis, OR 97331, USA. firstname.lastname@example.org