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Remodulation of central carbon metabolic pathway in response to arsenite exposure in Rhodococcus sp. strain NAU-1.

Jain R, Adhikary H, Jha S, Jha A, Kumar GN - Microb Biotechnol (2012)

Bottom Line: Assays confirmed the presence of membrane bound arsenite oxidase and cytoplasmic arsenate reductase in NAU-1.Specific activities of antioxidant enzymes (catalase, ascorbate peroxidase, superoxide dismutase and glutathione S-transferase) increased in dose-dependent manner with arsenite, whereas glutathione reductase activity decreased with increase in As(III) concentration.Enzyme assays revealed the increase in direct oxidative and glyoxylate pathway in Rhodococcus NAU-1 in the presence of As(III).

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, ASPEE College of Horticulture and Forestry, Navsari Agricultural University, Navsari, Gujarat 396456, India.

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Metabolic processes of Rhodococcus sp. strain NAU‐1 in the presence of AsIII. Complete ovals around enzyme names indicate the enzymes which are induced in the presence of arsenite. Dashed ovals around enzyme names indicate repressed enzymes in the presence of arsenite.
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f4: Metabolic processes of Rhodococcus sp. strain NAU‐1 in the presence of AsIII. Complete ovals around enzyme names indicate the enzymes which are induced in the presence of arsenite. Dashed ovals around enzyme names indicate repressed enzymes in the presence of arsenite.

Mentions: Tolerance to the toxic arsenite by microorganisms is extensively documented (Oremland et al., 2004; Achour et al., 2007; Chang et al., 2008; Lioa et al., 2011). Microorganisms cope up with the toxic effects of arsenite either by restricting its entry inside the cell or by converting it to less toxic form and immobilizing (Hughes, 2002). ars operon, one of the arsenic detoxification mechanisms, is mainly attributed to tolerance of arsenite in microorganisms. The present study is an attempt to isolate arsenite‐tolerant microorganisms, and to extend our knowledge beyond current ars gene‐mediated resistance models. The relative contribution of multiple gene products, and central carbon metabolic pathways were analysed in arsenite‐tolerant Rhodococcus sp. strain NAU‐1. Interestingly, the present study revealed remodulation of central metabolism in favour of arsenite tolerance (Fig. 4).


Remodulation of central carbon metabolic pathway in response to arsenite exposure in Rhodococcus sp. strain NAU-1.

Jain R, Adhikary H, Jha S, Jha A, Kumar GN - Microb Biotechnol (2012)

Metabolic processes of Rhodococcus sp. strain NAU‐1 in the presence of AsIII. Complete ovals around enzyme names indicate the enzymes which are induced in the presence of arsenite. Dashed ovals around enzyme names indicate repressed enzymes in the presence of arsenite.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815897&req=5

f4: Metabolic processes of Rhodococcus sp. strain NAU‐1 in the presence of AsIII. Complete ovals around enzyme names indicate the enzymes which are induced in the presence of arsenite. Dashed ovals around enzyme names indicate repressed enzymes in the presence of arsenite.
Mentions: Tolerance to the toxic arsenite by microorganisms is extensively documented (Oremland et al., 2004; Achour et al., 2007; Chang et al., 2008; Lioa et al., 2011). Microorganisms cope up with the toxic effects of arsenite either by restricting its entry inside the cell or by converting it to less toxic form and immobilizing (Hughes, 2002). ars operon, one of the arsenic detoxification mechanisms, is mainly attributed to tolerance of arsenite in microorganisms. The present study is an attempt to isolate arsenite‐tolerant microorganisms, and to extend our knowledge beyond current ars gene‐mediated resistance models. The relative contribution of multiple gene products, and central carbon metabolic pathways were analysed in arsenite‐tolerant Rhodococcus sp. strain NAU‐1. Interestingly, the present study revealed remodulation of central metabolism in favour of arsenite tolerance (Fig. 4).

Bottom Line: Assays confirmed the presence of membrane bound arsenite oxidase and cytoplasmic arsenate reductase in NAU-1.Specific activities of antioxidant enzymes (catalase, ascorbate peroxidase, superoxide dismutase and glutathione S-transferase) increased in dose-dependent manner with arsenite, whereas glutathione reductase activity decreased with increase in As(III) concentration.Enzyme assays revealed the increase in direct oxidative and glyoxylate pathway in Rhodococcus NAU-1 in the presence of As(III).

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, ASPEE College of Horticulture and Forestry, Navsari Agricultural University, Navsari, Gujarat 396456, India.

Show MeSH
Related in: MedlinePlus