Rapid detection of total and viable Legionella pneumophila in tap water by immunomagnetic separation, double fluorescent staining and flow cytometry.
Bottom Line: The recovery is over 85% of spiked Lp SG 1 cells in 1 l of tap water and detection limits are around 50 and 15 cells per litre for total and viable Lp, respectively.In 53% of the water samples from different taps and showers significantly higher concentrations of Lp were detected by flow cytometry.No correlation to the plate culture method was found.
Affiliation: Swiss Federal Institute for Aquatic Science and Technology (Eawag), Überlandstrasse 133, CH-8600, Dübendorf, Switzerland.Show MeSH
Mentions: For comparison, a total of 85 drinking water samples from different taps and showers were analysed by both plating and FCM. Laboratories 1 and 2 provided 45 samples and applied the ISO 11731 method (ISO, 2004), whereas Laboratory 3 provided 40 samples and did plate 1 ml of sample only. An overview of the results obtained is shown in Fig. 4.
Affiliation: Swiss Federal Institute for Aquatic Science and Technology (Eawag), Überlandstrasse 133, CH-8600, Dübendorf, Switzerland.