Limits...
Rapid detection of total and viable Legionella pneumophila in tap water by immunomagnetic separation, double fluorescent staining and flow cytometry.

Keserue HA, Baumgartner A, Felleisen R, Egli T - Microb Biotechnol (2012)

Bottom Line: The recovery is over 85% of spiked Lp SG 1 cells in 1 l of tap water and detection limits are around 50 and 15 cells per litre for total and viable Lp, respectively.In 53% of the water samples from different taps and showers significantly higher concentrations of Lp were detected by flow cytometry.No correlation to the plate culture method was found.

View Article: PubMed Central - PubMed

Affiliation: Swiss Federal Institute for Aquatic Science and Technology (Eawag), Überlandstrasse 133, CH-8600, Dübendorf, Switzerland.

Show MeSH
Comparison of tap water samples with naturally occurring Lp cells measured by plating and FCM. Laboratory 1 and 2 performed the complete ISO 11731 method whereas Laboratory 3 plated 1 ml only. Around 50% of the samples have significantly (> 0.5 log) higher total and viable FCM Lp counts than the plating.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3815896&req=5

f4: Comparison of tap water samples with naturally occurring Lp cells measured by plating and FCM. Laboratory 1 and 2 performed the complete ISO 11731 method whereas Laboratory 3 plated 1 ml only. Around 50% of the samples have significantly (> 0.5 log) higher total and viable FCM Lp counts than the plating.

Mentions: For comparison, a total of 85 drinking water samples from different taps and showers were analysed by both plating and FCM. Laboratories 1 and 2 provided 45 samples and applied the ISO 11731 method (ISO, 2004), whereas Laboratory 3 provided 40 samples and did plate 1 ml of sample only. An overview of the results obtained is shown in Fig. 4.


Rapid detection of total and viable Legionella pneumophila in tap water by immunomagnetic separation, double fluorescent staining and flow cytometry.

Keserue HA, Baumgartner A, Felleisen R, Egli T - Microb Biotechnol (2012)

Comparison of tap water samples with naturally occurring Lp cells measured by plating and FCM. Laboratory 1 and 2 performed the complete ISO 11731 method whereas Laboratory 3 plated 1 ml only. Around 50% of the samples have significantly (> 0.5 log) higher total and viable FCM Lp counts than the plating.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815896&req=5

f4: Comparison of tap water samples with naturally occurring Lp cells measured by plating and FCM. Laboratory 1 and 2 performed the complete ISO 11731 method whereas Laboratory 3 plated 1 ml only. Around 50% of the samples have significantly (> 0.5 log) higher total and viable FCM Lp counts than the plating.
Mentions: For comparison, a total of 85 drinking water samples from different taps and showers were analysed by both plating and FCM. Laboratories 1 and 2 provided 45 samples and applied the ISO 11731 method (ISO, 2004), whereas Laboratory 3 provided 40 samples and did plate 1 ml of sample only. An overview of the results obtained is shown in Fig. 4.

Bottom Line: The recovery is over 85% of spiked Lp SG 1 cells in 1 l of tap water and detection limits are around 50 and 15 cells per litre for total and viable Lp, respectively.In 53% of the water samples from different taps and showers significantly higher concentrations of Lp were detected by flow cytometry.No correlation to the plate culture method was found.

View Article: PubMed Central - PubMed

Affiliation: Swiss Federal Institute for Aquatic Science and Technology (Eawag), Überlandstrasse 133, CH-8600, Dübendorf, Switzerland.

Show MeSH