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Microbial community structure and dynamics in anaerobic fluidized-bed and granular sludge-bed reactors: influence of operational temperature and reactor configuration.

Bialek K, Kumar A, Mahony T, Lens PN, O'Flaherty V - Microb Biotechnol (2012)

Bottom Line: A comparable level of performance was recorded for both systems at 37°C and 25°C, but a more dynamic and diverse microbial community in the IFB reactors supported better stability and adaptative capacity towards low temperature operation.The emergence and maintenance of particular bacterial genotypes (phylum Firmicutes and Bacteroidetes) was associated with efficient protein hydrolysis in the IFB, while protein hydrolysis was inefficient in the EGSB.A significant community shift from a Methanobacteriales and Methanosaetaceae towards a Methanomicrobiales-predominated community was demonstrated during operation at 15°C in both reactor configurations.

View Article: PubMed Central - PubMed

Affiliation: Microbial Ecology Laboratory, Microbiology, School of Natural Sciences and Ryan Institute, National University of Ireland, Galway, Ireland.

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Neighbour‐joining tree illustrating the phylogenetic affiliations of the 16S rRNA gene sequences obtained from: (A) bacterial DGGE bands B1–B30 (accession numbers: JF927800–JF927829) and (B) archaeal DGGE bands A1–A9 (accession numbers: JF952003–JF952011). Reactor biomasses containing the respective bands are given in parenthesis.
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f3: Neighbour‐joining tree illustrating the phylogenetic affiliations of the 16S rRNA gene sequences obtained from: (A) bacterial DGGE bands B1–B30 (accession numbers: JF927800–JF927829) and (B) archaeal DGGE bands A1–A9 (accession numbers: JF952003–JF952011). Reactor biomasses containing the respective bands are given in parenthesis.

Mentions: In total, 30 DGGE bands, designated as B1–B30, were retrieved from the bacterial DGGE gel and used for subsequent sequencing analysis. Ten bands out of the 30 (B1, B2, B7, B10, B11, B16, B18, B19, B20, B24) were unique to the IFB reactor and 15 common bands were noted between the IFB and EGSB reactors (Table 1; Fig. 3A). In the archaeal DGGE gel, a total of 9 bands (A1–A9) were retrieved and sequenced (Table 2; Fig. 3B).


Microbial community structure and dynamics in anaerobic fluidized-bed and granular sludge-bed reactors: influence of operational temperature and reactor configuration.

Bialek K, Kumar A, Mahony T, Lens PN, O'Flaherty V - Microb Biotechnol (2012)

Neighbour‐joining tree illustrating the phylogenetic affiliations of the 16S rRNA gene sequences obtained from: (A) bacterial DGGE bands B1–B30 (accession numbers: JF927800–JF927829) and (B) archaeal DGGE bands A1–A9 (accession numbers: JF952003–JF952011). Reactor biomasses containing the respective bands are given in parenthesis.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815895&req=5

f3: Neighbour‐joining tree illustrating the phylogenetic affiliations of the 16S rRNA gene sequences obtained from: (A) bacterial DGGE bands B1–B30 (accession numbers: JF927800–JF927829) and (B) archaeal DGGE bands A1–A9 (accession numbers: JF952003–JF952011). Reactor biomasses containing the respective bands are given in parenthesis.
Mentions: In total, 30 DGGE bands, designated as B1–B30, were retrieved from the bacterial DGGE gel and used for subsequent sequencing analysis. Ten bands out of the 30 (B1, B2, B7, B10, B11, B16, B18, B19, B20, B24) were unique to the IFB reactor and 15 common bands were noted between the IFB and EGSB reactors (Table 1; Fig. 3A). In the archaeal DGGE gel, a total of 9 bands (A1–A9) were retrieved and sequenced (Table 2; Fig. 3B).

Bottom Line: A comparable level of performance was recorded for both systems at 37°C and 25°C, but a more dynamic and diverse microbial community in the IFB reactors supported better stability and adaptative capacity towards low temperature operation.The emergence and maintenance of particular bacterial genotypes (phylum Firmicutes and Bacteroidetes) was associated with efficient protein hydrolysis in the IFB, while protein hydrolysis was inefficient in the EGSB.A significant community shift from a Methanobacteriales and Methanosaetaceae towards a Methanomicrobiales-predominated community was demonstrated during operation at 15°C in both reactor configurations.

View Article: PubMed Central - PubMed

Affiliation: Microbial Ecology Laboratory, Microbiology, School of Natural Sciences and Ryan Institute, National University of Ireland, Galway, Ireland.

Show MeSH