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Identification of a novel Baeyer-Villiger monooxygenase from Acinetobacter radioresistens: close relationship to the Mycobacterium tuberculosis prodrug activator EtaA.

Minerdi D, Zgrablic I, Sadeghi SJ, Gilardi G - Microb Biotechnol (2012)

Bottom Line: Phylogenetic analysis placed the sequence of this novel BVMO in the same clade of the prodrug activator ethionamide monooxygenase (EtaA) and it bears only a distant relation to the other known class I BVMO proteins.In silico analysis of the 3D model of the S13 BVMO generated by homology modelling also supports the similarities with EtaA by binding ethionamide to the active site.In vitro experiments carried out with the purified enzyme confirm that this novel BVMO is indeed capable of typical Baeyer-Villiger reactions as well as oxidation of the prodrug ethionamide.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Sciences and Systems Biology, University of Torino, via Accademia Albertina 13, 10123 Torino, Italy.

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Growth of Acinetobacter radioresistens strain S13 on n‐alkanes. (A) Growth curve of Acinetobacter radioresistens strain S13 isolate in minimal medium (circle) supplemented with C14 (square) and C16 (triangle) during the first 96 h, (B) bacterial growth was followed by measuring OD at 600 nm after 36 h in the case of the liquid, medium‐length chain C14 and C16 alkanes and (C) viable cells number per millilitre of cultures when the bacteria were grown on minimal medium supplemented with the solid, long‐length chain C24 and C36 alkanes after 3 and 6 days respectively. CTRL = control experiments with bacterial culture grown on minimal medium without n‐alkanes.
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f2: Growth of Acinetobacter radioresistens strain S13 on n‐alkanes. (A) Growth curve of Acinetobacter radioresistens strain S13 isolate in minimal medium (circle) supplemented with C14 (square) and C16 (triangle) during the first 96 h, (B) bacterial growth was followed by measuring OD at 600 nm after 36 h in the case of the liquid, medium‐length chain C14 and C16 alkanes and (C) viable cells number per millilitre of cultures when the bacteria were grown on minimal medium supplemented with the solid, long‐length chain C24 and C36 alkanes after 3 and 6 days respectively. CTRL = control experiments with bacterial culture grown on minimal medium without n‐alkanes.

Mentions: The ability of A. radioresistens S13 to grow in liquid mineral salts medium (MSM) cultures supplemented with alkanes of defined carbon chain lengths was investigated. The bacterial growth on MSM medium supplemented with medium length liquid (C14 and C16) and long length solid (C24 and C36) alkanes was studied (Fig. 2).


Identification of a novel Baeyer-Villiger monooxygenase from Acinetobacter radioresistens: close relationship to the Mycobacterium tuberculosis prodrug activator EtaA.

Minerdi D, Zgrablic I, Sadeghi SJ, Gilardi G - Microb Biotechnol (2012)

Growth of Acinetobacter radioresistens strain S13 on n‐alkanes. (A) Growth curve of Acinetobacter radioresistens strain S13 isolate in minimal medium (circle) supplemented with C14 (square) and C16 (triangle) during the first 96 h, (B) bacterial growth was followed by measuring OD at 600 nm after 36 h in the case of the liquid, medium‐length chain C14 and C16 alkanes and (C) viable cells number per millilitre of cultures when the bacteria were grown on minimal medium supplemented with the solid, long‐length chain C24 and C36 alkanes after 3 and 6 days respectively. CTRL = control experiments with bacterial culture grown on minimal medium without n‐alkanes.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815892&req=5

f2: Growth of Acinetobacter radioresistens strain S13 on n‐alkanes. (A) Growth curve of Acinetobacter radioresistens strain S13 isolate in minimal medium (circle) supplemented with C14 (square) and C16 (triangle) during the first 96 h, (B) bacterial growth was followed by measuring OD at 600 nm after 36 h in the case of the liquid, medium‐length chain C14 and C16 alkanes and (C) viable cells number per millilitre of cultures when the bacteria were grown on minimal medium supplemented with the solid, long‐length chain C24 and C36 alkanes after 3 and 6 days respectively. CTRL = control experiments with bacterial culture grown on minimal medium without n‐alkanes.
Mentions: The ability of A. radioresistens S13 to grow in liquid mineral salts medium (MSM) cultures supplemented with alkanes of defined carbon chain lengths was investigated. The bacterial growth on MSM medium supplemented with medium length liquid (C14 and C16) and long length solid (C24 and C36) alkanes was studied (Fig. 2).

Bottom Line: Phylogenetic analysis placed the sequence of this novel BVMO in the same clade of the prodrug activator ethionamide monooxygenase (EtaA) and it bears only a distant relation to the other known class I BVMO proteins.In silico analysis of the 3D model of the S13 BVMO generated by homology modelling also supports the similarities with EtaA by binding ethionamide to the active site.In vitro experiments carried out with the purified enzyme confirm that this novel BVMO is indeed capable of typical Baeyer-Villiger reactions as well as oxidation of the prodrug ethionamide.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Sciences and Systems Biology, University of Torino, via Accademia Albertina 13, 10123 Torino, Italy.

Show MeSH
Related in: MedlinePlus