PEGylation of bacteriophages increases blood circulation time and reduces T-helper type 1 immune response.
Bottom Line: When injected into naïve mice, PEGylated phages showed a strong increase in circulation half-life, whereas challenge of immunized mice did not reveal a significant difference.Our results suggest that the prolonged half-life is due to decreased susceptibility to innate immunity as well as avoidance of cellular defence mechanisms.PEGylated viruses elicited significantly reduced levels of T-helper type 1-associated cytokine release (IFN-γ and IL-6), in both naïve and immunized mice.
Affiliation: Institute of Food Science and Nutrition, ETH Zurich, Schmelzbergstrasse 7, 8092 Zurich, Switzerland.Show MeSH
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Mentions: PEGylation of the phage particles did not significantly alter the mass or relative proportion of structural proteins, as demonstrated by SDS‐PAGE analysis (Fig. 2A). Some possible but minor changes in A511 proteins were observed after silver staining, mostly in the higher molecular mass range of the phage protein profile (Fig. 2A).
Affiliation: Institute of Food Science and Nutrition, ETH Zurich, Schmelzbergstrasse 7, 8092 Zurich, Switzerland.