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Expression of native and mutant extracellular lipases fromYarrowia lipolytica in Saccharomyces cerevisiae.

Darvishi F - Microb Biotechnol (2012)

Bottom Line: These strains can utilize olive oil and lipids as low-cost substrates to produce bioethanol, single cell protein and other biotechnologically valuable products.The LIP2 gene of Y. lipolytica was expressed in S. cerevisiae as a heterologous protein without any modifications.Strong components of the Y. lipolytica expression/secretion system could be used for high-level production of recombinant proteins in S. cerevisiae.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Faculty of Science, University of Maragheh, Maragheh, 55181-83111, Iran. f.darvishi@ymail.com

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Multiple sequence alignment obtained with the ClustalW program of the extracellular lipase LIP2 from Y. lipolytica strains CLIB122 (GenBank Accession No. XP500282), DSM3286 (GenBank Accession No. ADL57414), and U6 (GenBank Accession No. ADL57415). Asterisks with grey boxes indicate that threonine at the positions 121 and 129 is replaced with isoleucine and serine for the mutant lipase of Y. lipolytica U6 mutant strain in comparison with the native lipase of wild‐type strains.
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f3: Multiple sequence alignment obtained with the ClustalW program of the extracellular lipase LIP2 from Y. lipolytica strains CLIB122 (GenBank Accession No. XP500282), DSM3286 (GenBank Accession No. ADL57414), and U6 (GenBank Accession No. ADL57415). Asterisks with grey boxes indicate that threonine at the positions 121 and 129 is replaced with isoleucine and serine for the mutant lipase of Y. lipolytica U6 mutant strain in comparison with the native lipase of wild‐type strains.

Mentions: The effects of these genetic changes on LIP2 expression must be analysed stepwise in a suitable host. To address this problem, the ORF regions in the mutant and native LIP2 genes were selected for expression in S. cerevisiae. Multiple sequence alignment obtained with ClustalW program of the extracellular lipase LIP2 from Y. lipolytica strains CLIB122, DSM3286 and U6 show that threonine at the positions 121 and 129 is replaced with isoleucine and serine in the mutant lipase of Y. lipolytica U6 mutant strain compared with the native lipase of wild‐type strains (Fig. 3).


Expression of native and mutant extracellular lipases fromYarrowia lipolytica in Saccharomyces cerevisiae.

Darvishi F - Microb Biotechnol (2012)

Multiple sequence alignment obtained with the ClustalW program of the extracellular lipase LIP2 from Y. lipolytica strains CLIB122 (GenBank Accession No. XP500282), DSM3286 (GenBank Accession No. ADL57414), and U6 (GenBank Accession No. ADL57415). Asterisks with grey boxes indicate that threonine at the positions 121 and 129 is replaced with isoleucine and serine for the mutant lipase of Y. lipolytica U6 mutant strain in comparison with the native lipase of wild‐type strains.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815875&req=5

f3: Multiple sequence alignment obtained with the ClustalW program of the extracellular lipase LIP2 from Y. lipolytica strains CLIB122 (GenBank Accession No. XP500282), DSM3286 (GenBank Accession No. ADL57414), and U6 (GenBank Accession No. ADL57415). Asterisks with grey boxes indicate that threonine at the positions 121 and 129 is replaced with isoleucine and serine for the mutant lipase of Y. lipolytica U6 mutant strain in comparison with the native lipase of wild‐type strains.
Mentions: The effects of these genetic changes on LIP2 expression must be analysed stepwise in a suitable host. To address this problem, the ORF regions in the mutant and native LIP2 genes were selected for expression in S. cerevisiae. Multiple sequence alignment obtained with ClustalW program of the extracellular lipase LIP2 from Y. lipolytica strains CLIB122, DSM3286 and U6 show that threonine at the positions 121 and 129 is replaced with isoleucine and serine in the mutant lipase of Y. lipolytica U6 mutant strain compared with the native lipase of wild‐type strains (Fig. 3).

Bottom Line: These strains can utilize olive oil and lipids as low-cost substrates to produce bioethanol, single cell protein and other biotechnologically valuable products.The LIP2 gene of Y. lipolytica was expressed in S. cerevisiae as a heterologous protein without any modifications.Strong components of the Y. lipolytica expression/secretion system could be used for high-level production of recombinant proteins in S. cerevisiae.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Faculty of Science, University of Maragheh, Maragheh, 55181-83111, Iran. f.darvishi@ymail.com

Show MeSH
Related in: MedlinePlus