Expression of native and mutant extracellular lipases fromYarrowia lipolytica in Saccharomyces cerevisiae.
Bottom Line: These strains can utilize olive oil and lipids as low-cost substrates to produce bioethanol, single cell protein and other biotechnologically valuable products.The LIP2 gene of Y. lipolytica was expressed in S. cerevisiae as a heterologous protein without any modifications.Strong components of the Y. lipolytica expression/secretion system could be used for high-level production of recombinant proteins in S. cerevisiae.
Affiliation: Department of Microbiology, Faculty of Science, University of Maragheh, Maragheh, 55181-83111, Iran. email@example.comShow MeSH
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Mentions: In this study, the S. cerevisiae expression vector p426GPD with strong constitutive glycerol phosphate dehydrogenase (GPD) promoter was used for LIP2 gene expression (Fig. 1B). The native LIP2 gene from Y. lipolytica DSM3286 and the mutant LIP2 gene from the mutant Y. lipolytica U6 were cloned into the vector without any modifications. The resulting constructs designated pFDP100 and pFDP101 contained the native and mutant LIP2 gene respectively (Fig. 1C and D). The vectors were transformed to the S. cerevisiae strain CEN.PK 113‐5D, and then, the new recombinant S. cerevisiae containing native and mutant extracellular lipase named S. cerevisiae FDS100 and FDS101 respectively.
Affiliation: Department of Microbiology, Faculty of Science, University of Maragheh, Maragheh, 55181-83111, Iran. firstname.lastname@example.org