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The presence and role of bacterial quorum sensing in activated sludge.

Chong G, Kimyon O, Rice SA, Kjelleberg S, Manefield M - Microb Biotechnol (2012)

Bottom Line: N-acylated-l-homoserine lactone and exoenzyme production were frequently observed among the isolates.N-acylated-l-homoserine lactones produced by this strain were identified and genes ahyI/R and chiA, encoding AHL production and response and chitinase activity respectively, were sequenced.These experiments provide insight into the relationship between AHL-mediated gene expression and exoenzyme activity in activated sludge and may ultimately create opportunities to improve sludge performance.

View Article: PubMed Central - PubMed

Affiliation: Centre for Marine Bio-Innovation, School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, 2052, Australia.

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Extracellular chitinase activity in activated sludge in response to hourly addition of N‐3‐oxo‐hexanoyl‐l‐homoserine lactone. A strong increase in chitinase activity is evident in response to AHL addition. Treatments were performed in triplicate and error bars represent standard deviation.
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f4: Extracellular chitinase activity in activated sludge in response to hourly addition of N‐3‐oxo‐hexanoyl‐l‐homoserine lactone. A strong increase in chitinase activity is evident in response to AHL addition. Treatments were performed in triplicate and error bars represent standard deviation.

Mentions: Elevated N‐3‐oxo‐hexanoyl‐l‐homoserine concentrations generated a rapid 10‐fold increase in extracellular chitinase activity between 60 and 90 min (Fig. 4). After 2 h the chitinase activity began to decrease and resembled the untreated control after 4 h. The cause of the drop in chitinase production is currently unknown, although may relate to upregulation of acylase or lactonase activities. None of the remaining phenotypes tested showed any response to exogenous N‐3‐oxo‐hexanoyl‐l‐homoserine lactone addition.


The presence and role of bacterial quorum sensing in activated sludge.

Chong G, Kimyon O, Rice SA, Kjelleberg S, Manefield M - Microb Biotechnol (2012)

Extracellular chitinase activity in activated sludge in response to hourly addition of N‐3‐oxo‐hexanoyl‐l‐homoserine lactone. A strong increase in chitinase activity is evident in response to AHL addition. Treatments were performed in triplicate and error bars represent standard deviation.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815874&req=5

f4: Extracellular chitinase activity in activated sludge in response to hourly addition of N‐3‐oxo‐hexanoyl‐l‐homoserine lactone. A strong increase in chitinase activity is evident in response to AHL addition. Treatments were performed in triplicate and error bars represent standard deviation.
Mentions: Elevated N‐3‐oxo‐hexanoyl‐l‐homoserine concentrations generated a rapid 10‐fold increase in extracellular chitinase activity between 60 and 90 min (Fig. 4). After 2 h the chitinase activity began to decrease and resembled the untreated control after 4 h. The cause of the drop in chitinase production is currently unknown, although may relate to upregulation of acylase or lactonase activities. None of the remaining phenotypes tested showed any response to exogenous N‐3‐oxo‐hexanoyl‐l‐homoserine lactone addition.

Bottom Line: N-acylated-l-homoserine lactone and exoenzyme production were frequently observed among the isolates.N-acylated-l-homoserine lactones produced by this strain were identified and genes ahyI/R and chiA, encoding AHL production and response and chitinase activity respectively, were sequenced.These experiments provide insight into the relationship between AHL-mediated gene expression and exoenzyme activity in activated sludge and may ultimately create opportunities to improve sludge performance.

View Article: PubMed Central - PubMed

Affiliation: Centre for Marine Bio-Innovation, School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, 2052, Australia.

Show MeSH
Related in: MedlinePlus