The presence and role of bacterial quorum sensing in activated sludge.
Bottom Line: N-acylated-l-homoserine lactone and exoenzyme production were frequently observed among the isolates.N-acylated-l-homoserine lactones produced by this strain were identified and genes ahyI/R and chiA, encoding AHL production and response and chitinase activity respectively, were sequenced.These experiments provide insight into the relationship between AHL-mediated gene expression and exoenzyme activity in activated sludge and may ultimately create opportunities to improve sludge performance.
Affiliation: Centre for Marine Bio-Innovation, School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, 2052, Australia.Show MeSH
Related in: MedlinePlus
Mentions: Elevated N‐3‐oxo‐hexanoyl‐l‐homoserine concentrations generated a rapid 10‐fold increase in extracellular chitinase activity between 60 and 90 min (Fig. 4). After 2 h the chitinase activity began to decrease and resembled the untreated control after 4 h. The cause of the drop in chitinase production is currently unknown, although may relate to upregulation of acylase or lactonase activities. None of the remaining phenotypes tested showed any response to exogenous N‐3‐oxo‐hexanoyl‐l‐homoserine lactone addition.
Affiliation: Centre for Marine Bio-Innovation, School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, 2052, Australia.