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Streptomyces lividans inhibits the proliferation of the fungus Verticillium dahliae on seeds and roots of Arabidopsis thaliana.

Meschke H, Schrempf H - Microb Biotechnol (2010)

Bottom Line: The extension of hyphae from each individual strain correlates with the reduction of the pectin-containing mucilage-layer.As a result, plants have considerably reduced disease symptoms.As spores of the beneficial S. lividans strain are obtainable in large quantity, its application is highly attractive.

View Article: PubMed Central - PubMed

Affiliation: FB Biology/Chemistry, Applied Genetics of Microorganisms, University of Osnabrück, Barbarastr. 13, 49069 Osnabrück, Germany.

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Interactions within soil cultures. Within soil seeds of A. thaliana were inoculated for 40 h either with spores of S. lividans (A and D), with conidia of V. dahliae (B and E) or with spores of S. lividans together with conidia of V. dahliae (C and F). After incubation for 40 h, seeds including the attaching soil particles were stained with SYTO9 and Calcofluor (see Experimental procedures). Fluorescence corresponding to either Calcofluor (D–F) or both of them (A–C) were recorded. Hyphae of S. lividans () or of V. dahliae () are marked.
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f7: Interactions within soil cultures. Within soil seeds of A. thaliana were inoculated for 40 h either with spores of S. lividans (A and D), with conidia of V. dahliae (B and E) or with spores of S. lividans together with conidia of V. dahliae (C and F). After incubation for 40 h, seeds including the attaching soil particles were stained with SYTO9 and Calcofluor (see Experimental procedures). Fluorescence corresponding to either Calcofluor (D–F) or both of them (A–C) were recorded. Hyphae of S. lividans () or of V. dahliae () are marked.

Mentions: As the natural habitat of the fungus is the rhizosphere, additional studies were done in soil. Streptomyces lividans spores within soil germinated and developed to a network of hyphae (44 h, Fig. 7A), a large portion of which attached closely to the mucilage of the A. thaliana seed. Hyphae were found to be only clearly detectable after staining within the complex soil structure. As outlined under the first chapter, the application of two different fluorescent dye‐types (SYTO9 and Calcofluor) allows to distinguish the bacterial and fungal hpyhae. The S. lividans hyphae were stainable with the dye SYTO9 (Fig. 7A), but not with Calcofluor (Fig. 7D). Also, V. dahliae conidia proliferated well during 44 h to hyphae at and close to the seed mucilage within the soil. The fungal hyphae interacted with Calcofluor (Fig. 7E), but barely with SYTO9 (Fig. 7B). The simultaneous inoculation of S. lividans and V. dahliae led to an intensive growth of S. lividans at and next to the seed mucilage, which correlated with a lack of fungal development (Fig. 7C and F).


Streptomyces lividans inhibits the proliferation of the fungus Verticillium dahliae on seeds and roots of Arabidopsis thaliana.

Meschke H, Schrempf H - Microb Biotechnol (2010)

Interactions within soil cultures. Within soil seeds of A. thaliana were inoculated for 40 h either with spores of S. lividans (A and D), with conidia of V. dahliae (B and E) or with spores of S. lividans together with conidia of V. dahliae (C and F). After incubation for 40 h, seeds including the attaching soil particles were stained with SYTO9 and Calcofluor (see Experimental procedures). Fluorescence corresponding to either Calcofluor (D–F) or both of them (A–C) were recorded. Hyphae of S. lividans () or of V. dahliae () are marked.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815809&req=5

f7: Interactions within soil cultures. Within soil seeds of A. thaliana were inoculated for 40 h either with spores of S. lividans (A and D), with conidia of V. dahliae (B and E) or with spores of S. lividans together with conidia of V. dahliae (C and F). After incubation for 40 h, seeds including the attaching soil particles were stained with SYTO9 and Calcofluor (see Experimental procedures). Fluorescence corresponding to either Calcofluor (D–F) or both of them (A–C) were recorded. Hyphae of S. lividans () or of V. dahliae () are marked.
Mentions: As the natural habitat of the fungus is the rhizosphere, additional studies were done in soil. Streptomyces lividans spores within soil germinated and developed to a network of hyphae (44 h, Fig. 7A), a large portion of which attached closely to the mucilage of the A. thaliana seed. Hyphae were found to be only clearly detectable after staining within the complex soil structure. As outlined under the first chapter, the application of two different fluorescent dye‐types (SYTO9 and Calcofluor) allows to distinguish the bacterial and fungal hpyhae. The S. lividans hyphae were stainable with the dye SYTO9 (Fig. 7A), but not with Calcofluor (Fig. 7D). Also, V. dahliae conidia proliferated well during 44 h to hyphae at and close to the seed mucilage within the soil. The fungal hyphae interacted with Calcofluor (Fig. 7E), but barely with SYTO9 (Fig. 7B). The simultaneous inoculation of S. lividans and V. dahliae led to an intensive growth of S. lividans at and next to the seed mucilage, which correlated with a lack of fungal development (Fig. 7C and F).

Bottom Line: The extension of hyphae from each individual strain correlates with the reduction of the pectin-containing mucilage-layer.As a result, plants have considerably reduced disease symptoms.As spores of the beneficial S. lividans strain are obtainable in large quantity, its application is highly attractive.

View Article: PubMed Central - PubMed

Affiliation: FB Biology/Chemistry, Applied Genetics of Microorganisms, University of Osnabrück, Barbarastr. 13, 49069 Osnabrück, Germany.

Show MeSH
Related in: MedlinePlus