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The 'LipoYeasts' project: using the oleaginous yeast Yarrowia lipolytica in combination with specific bacterial genes for the bioconversion of lipids, fats and oils into high-value products.

Sabirova JS, Haddouche R, Van Bogaert I, Mulaa F, Verstraete W, Timmis K, Schmidt-Dannert C, Nicaud J, Soetaert W - Microb Biotechnol (2011)

Bottom Line: The oleochemical industry is currently still dominated by conventional chemistry, with biotechnology only starting to play a more prominent role, primarily with respect to the biosurfactants or lipases, e.g. as detergents, or for biofuel production.A major bottleneck for all further biotechnological applications is the problem of the initial mobilization of cheap and vastly available lipid and oil substrates, which are then to be transformed into high-value biotechnological, nutritional or pharmacological products.Different lipid stocks (petroleum, alkane, vegetable oil, fatty acid) and combinations thereof are being assessed as substrates in combination with different mutant and recombinant strains of Y. lipolytica, in order to modulate the composition and yields of the produced added-value products.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioscience and Bioengineering, Ghent University, Ghent, Belgium. julia.sabirova@ugent.be

ABSTRACT
The oleochemical industry is currently still dominated by conventional chemistry, with biotechnology only starting to play a more prominent role, primarily with respect to the biosurfactants or lipases, e.g. as detergents, or for biofuel production. A major bottleneck for all further biotechnological applications is the problem of the initial mobilization of cheap and vastly available lipid and oil substrates, which are then to be transformed into high-value biotechnological, nutritional or pharmacological products. Under the EU-sponsored LipoYeasts project we are developing the oleaginous yeast Yarrowia lipolytica into a versatile and high-throughput microbial factory that, by use of specific enzymatic pathways from hydrocarbonoclastic bacteria, efficiently mobilizes lipids by directing its versatile lipid metabolism towards the production of industrially valuable lipid-derived compounds like wax esters (WE), isoprenoid-derived compounds (carotenoids, polyenic carotenoid ester), polyhydroxyalkanoates (PHAs) and free hydroxylated fatty acids (HFAs). Different lipid stocks (petroleum, alkane, vegetable oil, fatty acid) and combinations thereof are being assessed as substrates in combination with different mutant and recombinant strains of Y. lipolytica, in order to modulate the composition and yields of the produced added-value products.

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Related in: MedlinePlus

Fatty acid‐fatty alcohol (top) and isoprenoid WE (bottom).
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f2: Fatty acid‐fatty alcohol (top) and isoprenoid WE (bottom).

Mentions: Wax esters are esters of long‐chain aliphatic alcohols and fatty acids (Fig. 2) and are used as high‐performance lubricants for engines, transmission and hydraulic systems (Lea, 2002). They are also used in cosmetics, foods and pharmaceuticals. Currently, these compounds are produced primarily from mineral oils, although the production of biodegradable lubricants from renewable sources increasingly attracts interest (Goodstein, 2004). However, as the most accessible natural sources of WEs, whale sperm oil and jojoba oil, are too expensive for wide range use, researchers have explored microbial sources for the synthesis of bio‐WEs and thus some Gram‐negative bacteria such as, e.g. Acinetobacter, Alcanivorax and Marinobacter species have been reported to synthesize different types of WEs as carbon storage compounds (Kalscheuer, 2010).


The 'LipoYeasts' project: using the oleaginous yeast Yarrowia lipolytica in combination with specific bacterial genes for the bioconversion of lipids, fats and oils into high-value products.

Sabirova JS, Haddouche R, Van Bogaert I, Mulaa F, Verstraete W, Timmis K, Schmidt-Dannert C, Nicaud J, Soetaert W - Microb Biotechnol (2011)

Fatty acid‐fatty alcohol (top) and isoprenoid WE (bottom).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815794&req=5

f2: Fatty acid‐fatty alcohol (top) and isoprenoid WE (bottom).
Mentions: Wax esters are esters of long‐chain aliphatic alcohols and fatty acids (Fig. 2) and are used as high‐performance lubricants for engines, transmission and hydraulic systems (Lea, 2002). They are also used in cosmetics, foods and pharmaceuticals. Currently, these compounds are produced primarily from mineral oils, although the production of biodegradable lubricants from renewable sources increasingly attracts interest (Goodstein, 2004). However, as the most accessible natural sources of WEs, whale sperm oil and jojoba oil, are too expensive for wide range use, researchers have explored microbial sources for the synthesis of bio‐WEs and thus some Gram‐negative bacteria such as, e.g. Acinetobacter, Alcanivorax and Marinobacter species have been reported to synthesize different types of WEs as carbon storage compounds (Kalscheuer, 2010).

Bottom Line: The oleochemical industry is currently still dominated by conventional chemistry, with biotechnology only starting to play a more prominent role, primarily with respect to the biosurfactants or lipases, e.g. as detergents, or for biofuel production.A major bottleneck for all further biotechnological applications is the problem of the initial mobilization of cheap and vastly available lipid and oil substrates, which are then to be transformed into high-value biotechnological, nutritional or pharmacological products.Different lipid stocks (petroleum, alkane, vegetable oil, fatty acid) and combinations thereof are being assessed as substrates in combination with different mutant and recombinant strains of Y. lipolytica, in order to modulate the composition and yields of the produced added-value products.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioscience and Bioengineering, Ghent University, Ghent, Belgium. julia.sabirova@ugent.be

ABSTRACT
The oleochemical industry is currently still dominated by conventional chemistry, with biotechnology only starting to play a more prominent role, primarily with respect to the biosurfactants or lipases, e.g. as detergents, or for biofuel production. A major bottleneck for all further biotechnological applications is the problem of the initial mobilization of cheap and vastly available lipid and oil substrates, which are then to be transformed into high-value biotechnological, nutritional or pharmacological products. Under the EU-sponsored LipoYeasts project we are developing the oleaginous yeast Yarrowia lipolytica into a versatile and high-throughput microbial factory that, by use of specific enzymatic pathways from hydrocarbonoclastic bacteria, efficiently mobilizes lipids by directing its versatile lipid metabolism towards the production of industrially valuable lipid-derived compounds like wax esters (WE), isoprenoid-derived compounds (carotenoids, polyenic carotenoid ester), polyhydroxyalkanoates (PHAs) and free hydroxylated fatty acids (HFAs). Different lipid stocks (petroleum, alkane, vegetable oil, fatty acid) and combinations thereof are being assessed as substrates in combination with different mutant and recombinant strains of Y. lipolytica, in order to modulate the composition and yields of the produced added-value products.

Show MeSH
Related in: MedlinePlus