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Rectal single dose immunization of mice with Escherichia coli O157:H7 bacterial ghosts induces efficient humoral and cellular immune responses and protects against the lethal heterologous challenge.

Mayr UB, Kudela P, Atrasheuskaya A, Bukin E, Ignatyev G, Lubitz W - Microb Biotechnol (2011)

Bottom Line: All these applications required a booster after primary immunization to achieve protective immunity against the lethal challenge.BGs from EHEC O157:H7 were prepared by a combination of protein E-mediated cell lysis and expression of staphylococcal nuclease A guaranteeing the complete degradation of pathogen residual DNA.The lack of genetic material in the EHEC BGs vaccine abolished any potential hazard for horizontal gene transfer of plasmid encoded antibiotic resistance genes or pathogenic islands to the recipient's gut flora.

View Article: PubMed Central - PubMed

Affiliation: Bird-C GmbH&CoKG, A-3420 Kritzendorf, Austria.

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Stimulation of Ag‐specific cell proliferation after the immunizations of mice with EHEC O157:H7 (N°CIP 105282) BGs. Mice were immunized as described in Fig. 1. Proliferation of Ag‐specific cells was assessed after 4 days of in vitro restimulation of spleen cells in the presence of EHEC O157:H7 BGs (specific Ag); measles inactivated Ag (strain Edmonston, non‐specific Ag) or as a negative control spleen cells were left without stimulation (A). The stimulatory effect of the boost immunization significantly enhanced proliferation of Ag‐specific spleen cells compared with proliferation of Ag‐specific spleen cells observed after the single immunization with EHEC O157:H7 BGs (B). Data represent the mean of three independent experiments ± SD (three mice per time point). P‐values < 0.05 were considered significant and are indicated with asterisks (*P < 0.05; **P < 0.01; ***P < 0.001). a, day of the first immunization in all groups; b, day of the second immunization in groups B1 and D1; c, day of the challenge.
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f4: Stimulation of Ag‐specific cell proliferation after the immunizations of mice with EHEC O157:H7 (N°CIP 105282) BGs. Mice were immunized as described in Fig. 1. Proliferation of Ag‐specific cells was assessed after 4 days of in vitro restimulation of spleen cells in the presence of EHEC O157:H7 BGs (specific Ag); measles inactivated Ag (strain Edmonston, non‐specific Ag) or as a negative control spleen cells were left without stimulation (A). The stimulatory effect of the boost immunization significantly enhanced proliferation of Ag‐specific spleen cells compared with proliferation of Ag‐specific spleen cells observed after the single immunization with EHEC O157:H7 BGs (B). Data represent the mean of three independent experiments ± SD (three mice per time point). P‐values < 0.05 were considered significant and are indicated with asterisks (*P < 0.05; **P < 0.01; ***P < 0.001). a, day of the first immunization in all groups; b, day of the second immunization in groups B1 and D1; c, day of the challenge.

Mentions: Moreover, the single rectal administration of EHEC O157:H7 BGs (group A1) significantly stimulated proliferation of Ag‐specific cells already 21 days after immunization (Fig. 4A). The slightly increased proliferation of responder cells was detected in group B1 (two rectal immunizations EHEC O157:H7 BGs at day 0 and day 28) (Fig. 4A) compared with group A1 (single rectal immunization with EHEC O157:H7 BGs at day 0), but a significant difference was only observed 14 days after the second immunization (day 42 after the first immunization) and remained unchanged until the end of experiment (Fig. 4B). Mice from the control groups immunized with placebo (C1 and D1) showed only minor differences in the cell proliferation indexes of spleen cells within the whole observation period (Fig. 4A).


Rectal single dose immunization of mice with Escherichia coli O157:H7 bacterial ghosts induces efficient humoral and cellular immune responses and protects against the lethal heterologous challenge.

Mayr UB, Kudela P, Atrasheuskaya A, Bukin E, Ignatyev G, Lubitz W - Microb Biotechnol (2011)

Stimulation of Ag‐specific cell proliferation after the immunizations of mice with EHEC O157:H7 (N°CIP 105282) BGs. Mice were immunized as described in Fig. 1. Proliferation of Ag‐specific cells was assessed after 4 days of in vitro restimulation of spleen cells in the presence of EHEC O157:H7 BGs (specific Ag); measles inactivated Ag (strain Edmonston, non‐specific Ag) or as a negative control spleen cells were left without stimulation (A). The stimulatory effect of the boost immunization significantly enhanced proliferation of Ag‐specific spleen cells compared with proliferation of Ag‐specific spleen cells observed after the single immunization with EHEC O157:H7 BGs (B). Data represent the mean of three independent experiments ± SD (three mice per time point). P‐values < 0.05 were considered significant and are indicated with asterisks (*P < 0.05; **P < 0.01; ***P < 0.001). a, day of the first immunization in all groups; b, day of the second immunization in groups B1 and D1; c, day of the challenge.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3815788&req=5

f4: Stimulation of Ag‐specific cell proliferation after the immunizations of mice with EHEC O157:H7 (N°CIP 105282) BGs. Mice were immunized as described in Fig. 1. Proliferation of Ag‐specific cells was assessed after 4 days of in vitro restimulation of spleen cells in the presence of EHEC O157:H7 BGs (specific Ag); measles inactivated Ag (strain Edmonston, non‐specific Ag) or as a negative control spleen cells were left without stimulation (A). The stimulatory effect of the boost immunization significantly enhanced proliferation of Ag‐specific spleen cells compared with proliferation of Ag‐specific spleen cells observed after the single immunization with EHEC O157:H7 BGs (B). Data represent the mean of three independent experiments ± SD (three mice per time point). P‐values < 0.05 were considered significant and are indicated with asterisks (*P < 0.05; **P < 0.01; ***P < 0.001). a, day of the first immunization in all groups; b, day of the second immunization in groups B1 and D1; c, day of the challenge.
Mentions: Moreover, the single rectal administration of EHEC O157:H7 BGs (group A1) significantly stimulated proliferation of Ag‐specific cells already 21 days after immunization (Fig. 4A). The slightly increased proliferation of responder cells was detected in group B1 (two rectal immunizations EHEC O157:H7 BGs at day 0 and day 28) (Fig. 4A) compared with group A1 (single rectal immunization with EHEC O157:H7 BGs at day 0), but a significant difference was only observed 14 days after the second immunization (day 42 after the first immunization) and remained unchanged until the end of experiment (Fig. 4B). Mice from the control groups immunized with placebo (C1 and D1) showed only minor differences in the cell proliferation indexes of spleen cells within the whole observation period (Fig. 4A).

Bottom Line: All these applications required a booster after primary immunization to achieve protective immunity against the lethal challenge.BGs from EHEC O157:H7 were prepared by a combination of protein E-mediated cell lysis and expression of staphylococcal nuclease A guaranteeing the complete degradation of pathogen residual DNA.The lack of genetic material in the EHEC BGs vaccine abolished any potential hazard for horizontal gene transfer of plasmid encoded antibiotic resistance genes or pathogenic islands to the recipient's gut flora.

View Article: PubMed Central - PubMed

Affiliation: Bird-C GmbH&CoKG, A-3420 Kritzendorf, Austria.

Show MeSH
Related in: MedlinePlus