Attenuated Salmonella enteritidis E23 as a vehicle for the rectal delivery of DNA vaccine coding for HIV-1 polyepitope CTL immunogen.
Bottom Line: The gene coding for TCI protein was used to construct the eukaryotic expression plasmid pcDNA-TCI.Detectable antibodies were generated in 2 weeks after immunization and their level increased after second immunization.This study demonstrates that attenuated S. enteritidis E23 is an effective live vector for rectal delivery of the DNA vaccine pcDNA-TCI to generate humoral and T-cellular responses against HIV-1.
Affiliation: State Research Center of Virology and Biotechnology 'Vector', 630559 Koltsovo, Novosibirsk, Russia. email@example.comShow MeSH
Related in: MedlinePlus
Mentions: The T‐cell immune responses were ascertained by the presence of IFN‐γ‐producing lymphocytes detected by ELISPOT assay using peptides EPFRDYVDRF from p24, DRVIEVVQGAYRAIR from gp41 and CTEMEKEGKISKIGP from RT as HIV‐1‐specific antigens. In a murine system, these peptides are multiple class I molecules, which can be presented to CTL. Figure 6 demonstrates that immunization with S. enteritidis E23/pcDNA‐TCI triggers CTL responses in comparison with control groups. These data relate to day 35 (day 7 after the second immunization), when the immune response was the strongest.
Affiliation: State Research Center of Virology and Biotechnology 'Vector', 630559 Koltsovo, Novosibirsk, Russia. firstname.lastname@example.org