Attenuated Salmonella enteritidis E23 as a vehicle for the rectal delivery of DNA vaccine coding for HIV-1 polyepitope CTL immunogen.
Bottom Line: The gene coding for TCI protein was used to construct the eukaryotic expression plasmid pcDNA-TCI.Detectable antibodies were generated in 2 weeks after immunization and their level increased after second immunization.This study demonstrates that attenuated S. enteritidis E23 is an effective live vector for rectal delivery of the DNA vaccine pcDNA-TCI to generate humoral and T-cellular responses against HIV-1.
Affiliation: State Research Center of Virology and Biotechnology 'Vector', 630559 Koltsovo, Novosibirsk, Russia. firstname.lastname@example.orgShow MeSH
Related in: MedlinePlus
Mentions: Peyer's patches are the main colonization site of the attenuated S. enteritidis E23/pcDNA‐TCI and an important immunologically relevant site in the context of the mucosal responsiveness. To test an in vivo delivery of pcDNA‐TCI using attenuated Salmonella as a transgenic vehicle, total cellular RNA was isolated from mouse small intestinal Peyer's patches on day 3 after the immunization with attenuated S. enteritidis E23/pcDNA‐TCI and the TCI gene transcription was analysed by RT‐PCR. As it is shown in (Fig. 3, only a DNA fragment with a length of about 1191 bp was amplified from RNA of the mice immunized with S. enteritidis E23/pcDNA‐TCI. Meanwhile, there were no DNA fragments amplified from the RNA prior to reverse transcription with the same primers or from the RNA of the control mice immunized with S. enteritidis E23/pcDNA3.1. Murine β‐actin DNA fragment (330 bp) was amplified from all samples.
Affiliation: State Research Center of Virology and Biotechnology 'Vector', 630559 Koltsovo, Novosibirsk, Russia. email@example.com