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Attenuated Salmonella enteritidis E23 as a vehicle for the rectal delivery of DNA vaccine coding for HIV-1 polyepitope CTL immunogen.

Karpenko LI, Danilenko AV, Bazhan SI, Danilenko ED, Sysoeva GM, Kaplina ON, Volkova OY, Oreshkova SF, Ilyichev AA - Microb Biotechnol (2011)

Bottom Line: The gene coding for TCI protein was used to construct the eukaryotic expression plasmid pcDNA-TCI.Detectable antibodies were generated in 2 weeks after immunization and their level increased after second immunization.This study demonstrates that attenuated S. enteritidis E23 is an effective live vector for rectal delivery of the DNA vaccine pcDNA-TCI to generate humoral and T-cellular responses against HIV-1.

View Article: PubMed Central - PubMed

Affiliation: State Research Center of Virology and Biotechnology 'Vector', 630559 Koltsovo, Novosibirsk, Russia. lkarpenko@ngs.ru

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Design of the TCI immunogen, a candidate for use as an anti‐HIV‐1 vaccine: a general schematic. Bar patterns indicate the polyepitope CTL immunogen and the origin of the sequences. The positions of individual epitopes and their MHC restrictions (HLA‐A, B, Cw – human; H‐2a, b, d, f, k, p, u, q – mouse; Mamu‐A*01 –Macaca mulatta) are depicted as lines below the CTL immunogen. Th stands for helper epitopes. All included epitopes are highly conserved in the three main HIV‐1 subtypes A, B and C.
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f1: Design of the TCI immunogen, a candidate for use as an anti‐HIV‐1 vaccine: a general schematic. Bar patterns indicate the polyepitope CTL immunogen and the origin of the sequences. The positions of individual epitopes and their MHC restrictions (HLA‐A, B, Cw – human; H‐2a, b, d, f, k, p, u, q – mouse; Mamu‐A*01 –Macaca mulatta) are depicted as lines below the CTL immunogen. Th stands for helper epitopes. All included epitopes are highly conserved in the three main HIV‐1 subtypes A, B and C.

Mentions: A synthetic polyepitope T‐cell immunogen (TCI) was designed as a candidate DNA‐based vaccine against HIV‐1 with the emphasis on stimulating CTLs, which play an important role in preventing HIV infection and/or slowing the progression to AIDS. TCI includes fragments from the main virus proteins Env, Gag, Pol and Nef, which contains the epitopes inducing both CD8+ CTL and CD4+ Th (Bazhan et al., 2004). All included epitopes are highly conserved in the three main HIV‐1 subtypes A, B and C. To be able to detect CTL responses induced by a DNA vaccine in experimental animals, additional epitopes, restricted by mouse and Macaque rhesus MHC class I molecules, were included in the target immunogen. Resulting artificial protein (392 amino acids in length) contains over 80 CTL epitopes, many of which are overlapping and are totally restricted by 10 different HLA class I (HLA‐A, B, Cw) molecules (Fig. 1) (Bazhan et al., 2004).


Attenuated Salmonella enteritidis E23 as a vehicle for the rectal delivery of DNA vaccine coding for HIV-1 polyepitope CTL immunogen.

Karpenko LI, Danilenko AV, Bazhan SI, Danilenko ED, Sysoeva GM, Kaplina ON, Volkova OY, Oreshkova SF, Ilyichev AA - Microb Biotechnol (2011)

Design of the TCI immunogen, a candidate for use as an anti‐HIV‐1 vaccine: a general schematic. Bar patterns indicate the polyepitope CTL immunogen and the origin of the sequences. The positions of individual epitopes and their MHC restrictions (HLA‐A, B, Cw – human; H‐2a, b, d, f, k, p, u, q – mouse; Mamu‐A*01 –Macaca mulatta) are depicted as lines below the CTL immunogen. Th stands for helper epitopes. All included epitopes are highly conserved in the three main HIV‐1 subtypes A, B and C.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815784&req=5

f1: Design of the TCI immunogen, a candidate for use as an anti‐HIV‐1 vaccine: a general schematic. Bar patterns indicate the polyepitope CTL immunogen and the origin of the sequences. The positions of individual epitopes and their MHC restrictions (HLA‐A, B, Cw – human; H‐2a, b, d, f, k, p, u, q – mouse; Mamu‐A*01 –Macaca mulatta) are depicted as lines below the CTL immunogen. Th stands for helper epitopes. All included epitopes are highly conserved in the three main HIV‐1 subtypes A, B and C.
Mentions: A synthetic polyepitope T‐cell immunogen (TCI) was designed as a candidate DNA‐based vaccine against HIV‐1 with the emphasis on stimulating CTLs, which play an important role in preventing HIV infection and/or slowing the progression to AIDS. TCI includes fragments from the main virus proteins Env, Gag, Pol and Nef, which contains the epitopes inducing both CD8+ CTL and CD4+ Th (Bazhan et al., 2004). All included epitopes are highly conserved in the three main HIV‐1 subtypes A, B and C. To be able to detect CTL responses induced by a DNA vaccine in experimental animals, additional epitopes, restricted by mouse and Macaque rhesus MHC class I molecules, were included in the target immunogen. Resulting artificial protein (392 amino acids in length) contains over 80 CTL epitopes, many of which are overlapping and are totally restricted by 10 different HLA class I (HLA‐A, B, Cw) molecules (Fig. 1) (Bazhan et al., 2004).

Bottom Line: The gene coding for TCI protein was used to construct the eukaryotic expression plasmid pcDNA-TCI.Detectable antibodies were generated in 2 weeks after immunization and their level increased after second immunization.This study demonstrates that attenuated S. enteritidis E23 is an effective live vector for rectal delivery of the DNA vaccine pcDNA-TCI to generate humoral and T-cellular responses against HIV-1.

View Article: PubMed Central - PubMed

Affiliation: State Research Center of Virology and Biotechnology 'Vector', 630559 Koltsovo, Novosibirsk, Russia. lkarpenko@ngs.ru

Show MeSH
Related in: MedlinePlus