Relevant uses of surface proteins--display on self-organized biological structures.
Bottom Line: Proteins are often found attached to surfaces of self-assembling biological units such as whole microbial cells or subcellular structures, e.g. intracellular inclusions.For some diagnostic purposes phages are even too small in size so other carrier materials where needed and gave way for cell and yeast display.Only recently, intracellular inclusions such as magnetosomes, polyhydroxyalkanoate granules and lipid bodies were conceived as stable subcellular structures enabling the display of foreign protein functions and showing potential as specific and tailor-made devices for medical and biotechnological applications.
Affiliation: Institute of Molecular BioSciences, Massey University, Private Bag 11222, Palmerston North, New Zealand.Show MeSH
Mentions: The flocculin system can also be applied following two different strategies. Either the GPI anchor of the C‐terminal half of the FLO1 protein is used and as described for the Agα1 system, the protein of interest is fused between the signal sequence and the anchoring motif. A second approach employs the adhesive ability of the flocculation domain of FLO1, a fusion of this domain to the N‐terminus of the protein of interest will non‐covalently bind the heterologous fusion protein to the cell wall via interaction with mannan chains (Kondo and Ueda, 2004). The latter system has been suggested to be advantageous for the display of enzymes with a C‐terminally located active site, e.g. lipases (Matsumoto et al., 2002) (Fig. 1).
Affiliation: Institute of Molecular BioSciences, Massey University, Private Bag 11222, Palmerston North, New Zealand.