A molecular key for building hyphae aggregates: the role of the newly identified Streptomyces protein HyaS.
Bottom Line: The HyaS protein is dominantly associated with the substrate hyphae.Investigations of ΔH transformants, each carrying a specifically mutated gene, lead to the conclusion that the in situ oxidase activity correlates with the pellet-inducing role of HyaS, and depends on the presence of certain histidine residues.These data present the first molecular basis for future manipulation of pellets, and concomitant production of secondary metabolites during biotechnological processes.
Affiliation: University of Osnabrück, FB Biology/Chemistry, Applied Genetics of Microorganisms, 49069 Osnabrück, Germany.Show MeSH
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Mentions: To investigate the role of the hyaS gene within the WT chromosome (Fig. 3, line D), we constructed several independent S. lividans mutants (named ΔH, Fig. 3, line E, and Table 1), which carried a hyaS gene disrupted by the hygromycin‐resistance cassette (Ωhyg) as outlined under Experimental procedures. On solid medium, ΔH colonies grew like the WT strain, and shared the differentiation pattern leading to spores (not shown). As viewed by transmission‐electron microscopy, the shape of the sectioned mutant spores corresponded to those of the WT strain (following Figs 4 and 5, the spores are presented in Fig. 6A and B).
Affiliation: University of Osnabrück, FB Biology/Chemistry, Applied Genetics of Microorganisms, 49069 Osnabrück, Germany.