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Arabinoxylan-oligosaccharides (AXOS) affect the protein/carbohydrate fermentation balance and microbial population dynamics of the Simulator of Human Intestinal Microbial Ecosystem.

Sanchez JI, Marzorati M, Grootaert C, Baran M, Van Craeyveld V, Courtin CM, Broekaert WF, Delcour JA, Verstraete W, Van de Wiele T - Microb Biotechnol (2008)

Bottom Line: Denaturant gradient gel electrophoresis (DGGE) analysis indicated that AXOS supplementation only slightly modified the total microbial community, implying that the observed effects on fermentation markers are mainly caused by changes in fermentation activity.Finally, specific quantitative PCR (qPCR) analysis showed that AXOS supplementation significantly increased the amount of health-promoting lactobacilli as well as of Bacteroides-Prevotella and Clostridium coccoides-Eubacterium rectale groups.These data allow concluding that AXOS are promising candidates to modulate the microbial metabolism in the distal colon.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Gent, Belgium.

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Related in: MedlinePlus

Concentrations of bifidobacteria (A), lactobacilli (B), Clostridium coccoides–Eubacterium rectale group (C) and Bacteroides–Prevotella group (D) in the intestinal suspension from the three colon vessels, derived from quantitative PCR. Black bars refer to the basal period; dark grey bars to the treatment period; light grey bars to the washout period. Below each bar is indicated a number, the percentage of the analysed group on the total of the microbial community. Asterisks indicate those bars that are significantly different from the basal period (P < 0.05).
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f3: Concentrations of bifidobacteria (A), lactobacilli (B), Clostridium coccoides–Eubacterium rectale group (C) and Bacteroides–Prevotella group (D) in the intestinal suspension from the three colon vessels, derived from quantitative PCR. Black bars refer to the basal period; dark grey bars to the treatment period; light grey bars to the washout period. Below each bar is indicated a number, the percentage of the analysed group on the total of the microbial community. Asterisks indicate those bars that are significantly different from the basal period (P < 0.05).

Mentions: The qPCR data on total bacteria (data not shown) confirmed the general increase in cell numbers observed through plate counts. AXOS supplementation induced an increase of the amount of both bifidobacteria and lactobacilli (Fig. 3). For bifidobacteria, a significant 2.2 times increase of the 16S rRNA gene copies per microlitre in the ascending colon was observed (P < 0.05), while, for lactobacilli, the increase was of four times in the ascending colon and of 1.3 and 1.8 times in the transverse and descending colon respectively. The increase in bifidobacteria concentrations did not persist after the end of the treatment period, while the lactobacilli concentration in the transverse colon kept increasing also when the AXOS supplementation ceased (P < 0.05). Moreover, as reported by other studies (Kabel et al., 2002; Chassard et al., 2007; Hughes et al., 2007) also bacteroides and clostridia groups took advantage of AXOS supplementation. The amount of microorganisms associated to C. coccoides–E. rectale group increased of 1.2 Log units after the treatment in the descending colon and also significantly slightly start to increase both in the ascending and in the descending colon during the treatment period (Fig. 3C). Bacteroides concentration significantly increased in all the three colon compartments becoming 65% of the total microbial community both in the transverse and in the descending colon during the washout period (Fig. 3D).


Arabinoxylan-oligosaccharides (AXOS) affect the protein/carbohydrate fermentation balance and microbial population dynamics of the Simulator of Human Intestinal Microbial Ecosystem.

Sanchez JI, Marzorati M, Grootaert C, Baran M, Van Craeyveld V, Courtin CM, Broekaert WF, Delcour JA, Verstraete W, Van de Wiele T - Microb Biotechnol (2008)

Concentrations of bifidobacteria (A), lactobacilli (B), Clostridium coccoides–Eubacterium rectale group (C) and Bacteroides–Prevotella group (D) in the intestinal suspension from the three colon vessels, derived from quantitative PCR. Black bars refer to the basal period; dark grey bars to the treatment period; light grey bars to the washout period. Below each bar is indicated a number, the percentage of the analysed group on the total of the microbial community. Asterisks indicate those bars that are significantly different from the basal period (P < 0.05).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815425&req=5

f3: Concentrations of bifidobacteria (A), lactobacilli (B), Clostridium coccoides–Eubacterium rectale group (C) and Bacteroides–Prevotella group (D) in the intestinal suspension from the three colon vessels, derived from quantitative PCR. Black bars refer to the basal period; dark grey bars to the treatment period; light grey bars to the washout period. Below each bar is indicated a number, the percentage of the analysed group on the total of the microbial community. Asterisks indicate those bars that are significantly different from the basal period (P < 0.05).
Mentions: The qPCR data on total bacteria (data not shown) confirmed the general increase in cell numbers observed through plate counts. AXOS supplementation induced an increase of the amount of both bifidobacteria and lactobacilli (Fig. 3). For bifidobacteria, a significant 2.2 times increase of the 16S rRNA gene copies per microlitre in the ascending colon was observed (P < 0.05), while, for lactobacilli, the increase was of four times in the ascending colon and of 1.3 and 1.8 times in the transverse and descending colon respectively. The increase in bifidobacteria concentrations did not persist after the end of the treatment period, while the lactobacilli concentration in the transverse colon kept increasing also when the AXOS supplementation ceased (P < 0.05). Moreover, as reported by other studies (Kabel et al., 2002; Chassard et al., 2007; Hughes et al., 2007) also bacteroides and clostridia groups took advantage of AXOS supplementation. The amount of microorganisms associated to C. coccoides–E. rectale group increased of 1.2 Log units after the treatment in the descending colon and also significantly slightly start to increase both in the ascending and in the descending colon during the treatment period (Fig. 3C). Bacteroides concentration significantly increased in all the three colon compartments becoming 65% of the total microbial community both in the transverse and in the descending colon during the washout period (Fig. 3D).

Bottom Line: Denaturant gradient gel electrophoresis (DGGE) analysis indicated that AXOS supplementation only slightly modified the total microbial community, implying that the observed effects on fermentation markers are mainly caused by changes in fermentation activity.Finally, specific quantitative PCR (qPCR) analysis showed that AXOS supplementation significantly increased the amount of health-promoting lactobacilli as well as of Bacteroides-Prevotella and Clostridium coccoides-Eubacterium rectale groups.These data allow concluding that AXOS are promising candidates to modulate the microbial metabolism in the distal colon.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Gent, Belgium.

Show MeSH
Related in: MedlinePlus