Arabinoxylan-oligosaccharides (AXOS) affect the protein/carbohydrate fermentation balance and microbial population dynamics of the Simulator of Human Intestinal Microbial Ecosystem.
Bottom Line: Denaturant gradient gel electrophoresis (DGGE) analysis indicated that AXOS supplementation only slightly modified the total microbial community, implying that the observed effects on fermentation markers are mainly caused by changes in fermentation activity.Finally, specific quantitative PCR (qPCR) analysis showed that AXOS supplementation significantly increased the amount of health-promoting lactobacilli as well as of Bacteroides-Prevotella and Clostridium coccoides-Eubacterium rectale groups.These data allow concluding that AXOS are promising candidates to modulate the microbial metabolism in the distal colon.
Affiliation: Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Gent, Belgium.Show MeSH
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Mentions: DGGE was used to monitor changes in the composition and structure of microbial communities from the three colon compartments, throughout the SHIME run of 7 weeks. Samples were collected at the end of every week from each of the three colon vessels. Clustering of the DGGE lane fingerprints (Fig. 2) showed that all samples from the ascending colon (V1) clustered in a separate group, and that the samples from the transverse (V2) and descending (V3) colon clustered together in another one. Within the transverse/descending colon group, samples from week 5 (end of the treatment) and weeks 6 and 7 (washout) formed separate subclusters (Fig. 2, dashed squares) indicating that the AXOS supplementation had impacted the composition of colonic microbial community in a way that remained stable also after the treatment. Among several small changes in the composition and intensity of the band patterns, a single band appeared to become dominant following AXOS supplementation (Fig. 2, black full square). Sequencing of the DNA eluted from the band identified a bacterial species with a similarity of 95% (on 164 bp) with Selenomonas sp. (AF385576).
Affiliation: Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Gent, Belgium.