A novel antimicrobial protein for plant protection consisting of a Xanthomonas oryzae harpin and active domains of cecropin A and melittin.
Bottom Line: The resulting chimeric protein maintained not only the HR-inducing property of the harpin, but also the antimicrobial activity of the cecropin A-melittin hybrid.Importantly, the protein acted as a potential pesticide by inducing disease resistance for viral, bacterial and fungal pathogens.This designed drug can be considered as a lead compound for use in plant protection, either for the development of new broad-spectrum pesticides or for expression in transgenic plants.
Affiliation: Department of Plant Pathology, Nanjing Agricultural University/Key Laboratory of Monitoring and Management for Plant Diseases and Insects, Ministry of Agriculture of China, Nanjing 210095, China.Show MeSH
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Mentions: To verify our earlier results using the CFEPs (Fig. 2), that the antibacterial activity of Hcm1 is due to the addition of the cecropin A‐melittin hybrid to the Hpa1 backbone, we used purified Hcm1 to test for growth inhibition of E. coli on LB plates, using Hpa1 at 5 µM as a negative control and kanamycin (Km) at 10 µg ml−1 as a positive control. Indeed, the application of 0.5 µM of Hcm1 to a sterile paper disc caused an obvious inhibition halo against E. coli BL21 (DE3), while Hpa1 did not (Fig. 4A), confirming that the addition of the cecropin A‐melittin hybrid at the C‐terminus of Hpa1 confers antibacterial activity to Hcm1.
Affiliation: Department of Plant Pathology, Nanjing Agricultural University/Key Laboratory of Monitoring and Management for Plant Diseases and Insects, Ministry of Agriculture of China, Nanjing 210095, China.