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Molecular and physiological properties of bacteriophages from North America and Germany affecting the fire blight pathogen Erwinia amylovora.

Müller I, Lurz R, Kube M, Quedenau C, Jelkmann W, Geider K - Microb Biotechnol (2011)

Bottom Line: The Myoviridae efficiently lysed their hosts and protected apple flowers significantly better than the Podoviridae against E. amylovora and should be preferred in biocontrol experiments.They belong to the Podoviridae or Myoviridae with a host range similar to the phages isolated in North America.In EM measurements, the genome sizes of the Podoviridae were smaller than the genomes of the Myoviridae from North America and from Germany, which differed from each other in corresponding nucleotide sequences.

View Article: PubMed Central - PubMed

Affiliation: Julius Kühn-Institut, Institut für Pflanzenschutz in Obst- und Weinbau, Schwabenheimer Str. 101, 69221 Dossenheim, Germany.

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Growth reduction of E. amylovora by phage treatment on apple flowers and immature pears. A. Flower assays with Ea1/79Sm using single bacteriophages. Control flowers were inoculated only with Ea1/79Sm cells. B. Fire blight symptoms on pear slices. Strain Ea1/79Sm applied without and with single bacteriophages.
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f5: Growth reduction of E. amylovora by phage treatment on apple flowers and immature pears. A. Flower assays with Ea1/79Sm using single bacteriophages. Control flowers were inoculated only with Ea1/79Sm cells. B. Fire blight symptoms on pear slices. Strain Ea1/79Sm applied without and with single bacteriophages.

Mentions: The Podoviridae had a weak effect on growth reduction of E. amylovora in flowers (Fig. 5A). φEa1h and φEa100 reduced the recovered pathogen by 40% compared with the control flowers. The MyoviridaeφEa104 and φEa116 reduced the recovered cells by 90%.


Molecular and physiological properties of bacteriophages from North America and Germany affecting the fire blight pathogen Erwinia amylovora.

Müller I, Lurz R, Kube M, Quedenau C, Jelkmann W, Geider K - Microb Biotechnol (2011)

Growth reduction of E. amylovora by phage treatment on apple flowers and immature pears. A. Flower assays with Ea1/79Sm using single bacteriophages. Control flowers were inoculated only with Ea1/79Sm cells. B. Fire blight symptoms on pear slices. Strain Ea1/79Sm applied without and with single bacteriophages.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815410&req=5

f5: Growth reduction of E. amylovora by phage treatment on apple flowers and immature pears. A. Flower assays with Ea1/79Sm using single bacteriophages. Control flowers were inoculated only with Ea1/79Sm cells. B. Fire blight symptoms on pear slices. Strain Ea1/79Sm applied without and with single bacteriophages.
Mentions: The Podoviridae had a weak effect on growth reduction of E. amylovora in flowers (Fig. 5A). φEa1h and φEa100 reduced the recovered pathogen by 40% compared with the control flowers. The MyoviridaeφEa104 and φEa116 reduced the recovered cells by 90%.

Bottom Line: The Myoviridae efficiently lysed their hosts and protected apple flowers significantly better than the Podoviridae against E. amylovora and should be preferred in biocontrol experiments.They belong to the Podoviridae or Myoviridae with a host range similar to the phages isolated in North America.In EM measurements, the genome sizes of the Podoviridae were smaller than the genomes of the Myoviridae from North America and from Germany, which differed from each other in corresponding nucleotide sequences.

View Article: PubMed Central - PubMed

Affiliation: Julius Kühn-Institut, Institut für Pflanzenschutz in Obst- und Weinbau, Schwabenheimer Str. 101, 69221 Dossenheim, Germany.

Show MeSH
Related in: MedlinePlus