Molecular and physiological properties of bacteriophages from North America and Germany affecting the fire blight pathogen Erwinia amylovora.
Bottom Line: The Myoviridae efficiently lysed their hosts and protected apple flowers significantly better than the Podoviridae against E. amylovora and should be preferred in biocontrol experiments.They belong to the Podoviridae or Myoviridae with a host range similar to the phages isolated in North America.In EM measurements, the genome sizes of the Podoviridae were smaller than the genomes of the Myoviridae from North America and from Germany, which differed from each other in corresponding nucleotide sequences.
Affiliation: Julius Kühn-Institut, Institut für Pflanzenschutz in Obst- und Weinbau, Schwabenheimer Str. 101, 69221 Dossenheim, Germany.Show MeSH
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Mentions: About 70% of the genome size of φEa116 as determined by EM were obtained by shotgun sequencing and primer walking using the complete genome sequence of the related phages φEa104 and φEa21‐4 for primer design. We assume strong viral promoters to cause lethal effects by cloning some insertions in E. coli. The relationship of φEa116 to φEa104 indicated a genome length of 85 kb, which is in agreement with EM data for the contour length of DNA from φEa116. The positions of the six contigs obtained are outlined in Fig. 3 and Table 2, with respect to the genome of the related phage φEa104. The contigs show 80–90% identity to the corresponding sequences of φEa104. Their annotation corresponds to parts of the annotated φEa104 genome. No EPS depolymerase gene was identified in the genome of both phages, which could indicate their EPS‐independent action for growth on E. amylovora strains without an amylovoran capsule.
Affiliation: Julius Kühn-Institut, Institut für Pflanzenschutz in Obst- und Weinbau, Schwabenheimer Str. 101, 69221 Dossenheim, Germany.