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Molecular and physiological properties of bacteriophages from North America and Germany affecting the fire blight pathogen Erwinia amylovora.

Müller I, Lurz R, Kube M, Quedenau C, Jelkmann W, Geider K - Microb Biotechnol (2011)

Bottom Line: The Myoviridae efficiently lysed their hosts and protected apple flowers significantly better than the Podoviridae against E. amylovora and should be preferred in biocontrol experiments.They belong to the Podoviridae or Myoviridae with a host range similar to the phages isolated in North America.In EM measurements, the genome sizes of the Podoviridae were smaller than the genomes of the Myoviridae from North America and from Germany, which differed from each other in corresponding nucleotide sequences.

View Article: PubMed Central - PubMed

Affiliation: Julius Kühn-Institut, Institut für Pflanzenschutz in Obst- und Weinbau, Schwabenheimer Str. 101, 69221 Dossenheim, Germany.

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Electron microscopic visualization of Erwinia amylovora phages. (A) φEa1h; (B) φEaJ08T; (C) φEaJ08C; (D) φEa100; (E) φEa08KT; (F) φEa116; (G) φEa104. The bar represents a size of 60 nm.
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f1: Electron microscopic visualization of Erwinia amylovora phages. (A) φEa1h; (B) φEaJ08T; (C) φEaJ08C; (D) φEa100; (E) φEa08KT; (F) φEa116; (G) φEa104. The bar represents a size of 60 nm.

Mentions: We have characterized four E. amylovora phages isolated in North America and three phages from Germany. The phages were allocated into morphotype groups according to Ackermann (2007). The American phages show an icosahedral head (size 60–73 nm, Table 1). The first group carries a short tail which was not seen in the contracted form (Fig. 1), and a second group of phages carries long tails of 114 nm (Table 1). This tail is contractile as shown in Fig. 1G for phage φEa104 and others. Erwinia amylovora phages φEa1h and φEa100 (Fig. 1A and D) are compact particles without an extended tail, whereas φEa104 and φEa116 have a well‐visible tail. In negative staining of the phage particles small tail fibres were detected, and genes encoding tail fibre proteins were identified on the genomes.


Molecular and physiological properties of bacteriophages from North America and Germany affecting the fire blight pathogen Erwinia amylovora.

Müller I, Lurz R, Kube M, Quedenau C, Jelkmann W, Geider K - Microb Biotechnol (2011)

Electron microscopic visualization of Erwinia amylovora phages. (A) φEa1h; (B) φEaJ08T; (C) φEaJ08C; (D) φEa100; (E) φEa08KT; (F) φEa116; (G) φEa104. The bar represents a size of 60 nm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815410&req=5

f1: Electron microscopic visualization of Erwinia amylovora phages. (A) φEa1h; (B) φEaJ08T; (C) φEaJ08C; (D) φEa100; (E) φEa08KT; (F) φEa116; (G) φEa104. The bar represents a size of 60 nm.
Mentions: We have characterized four E. amylovora phages isolated in North America and three phages from Germany. The phages were allocated into morphotype groups according to Ackermann (2007). The American phages show an icosahedral head (size 60–73 nm, Table 1). The first group carries a short tail which was not seen in the contracted form (Fig. 1), and a second group of phages carries long tails of 114 nm (Table 1). This tail is contractile as shown in Fig. 1G for phage φEa104 and others. Erwinia amylovora phages φEa1h and φEa100 (Fig. 1A and D) are compact particles without an extended tail, whereas φEa104 and φEa116 have a well‐visible tail. In negative staining of the phage particles small tail fibres were detected, and genes encoding tail fibre proteins were identified on the genomes.

Bottom Line: The Myoviridae efficiently lysed their hosts and protected apple flowers significantly better than the Podoviridae against E. amylovora and should be preferred in biocontrol experiments.They belong to the Podoviridae or Myoviridae with a host range similar to the phages isolated in North America.In EM measurements, the genome sizes of the Podoviridae were smaller than the genomes of the Myoviridae from North America and from Germany, which differed from each other in corresponding nucleotide sequences.

View Article: PubMed Central - PubMed

Affiliation: Julius Kühn-Institut, Institut für Pflanzenschutz in Obst- und Weinbau, Schwabenheimer Str. 101, 69221 Dossenheim, Germany.

Show MeSH
Related in: MedlinePlus