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The potential of bacteria isolated from ruminal contents of seaweed-eating North Ronaldsay sheep to hydrolyse seaweed components and produce methane by anaerobic digestion in vitro.

Williams AG, Withers S, Sutherland AD - Microb Biotechnol (2012)

Bottom Line: Only nine isolates out of 65 utilized >90% of the polysaccharide they were isolated on.The nine isolates (eight Prevotella spp. and one Clostridium butyricum) utilized whole Laminaria hyperborea extract and a range of seaweed polysaccharides, including alginate (seven out of nine isolates), laminarin and carboxymethylcellulose (eight out of nine isolates); while two out of nine isolates additionally hydrolysed fucoidan to some extent.Crude enzyme extracts from three of the isolates studied further had diverse glycosidases and polysaccharidase activities; particularly against laminarin and alginate (two isolates were shown to have alginate lyase activity) and notably fucoidan and carageenan (one isolate).

View Article: PubMed Central - PubMed

Affiliation: Department of Biological and Biomedical Sciences, School of Health and Life Sciences, Glasgow Caledonian University, Cowcaddens Road, Glasgow, G4 0BA, UK.

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Methane accumulation as monitored at the end of the 7-day consecutive incubation period for each of the serial cultures. The proportion of methane as a percentage of the headspace gas is indicated for various enrichment substrates at the end of week 1 () through to the end of week 6 (□).
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fig02: Methane accumulation as monitored at the end of the 7-day consecutive incubation period for each of the serial cultures. The proportion of methane as a percentage of the headspace gas is indicated for various enrichment substrates at the end of week 1 () through to the end of week 6 (□).

Mentions: Stable methanogenic cultures were established with structural polymers and storage polymers including mannitol (Fig. 2) confirming that the ability to catabolize a wide range of seaweed carbohydrates was present in the initial ruminal population. Although fucoidan was catabolized less effectively than other polysaccharides its utilization was also associated with consistent methane formation.


The potential of bacteria isolated from ruminal contents of seaweed-eating North Ronaldsay sheep to hydrolyse seaweed components and produce methane by anaerobic digestion in vitro.

Williams AG, Withers S, Sutherland AD - Microb Biotechnol (2012)

Methane accumulation as monitored at the end of the 7-day consecutive incubation period for each of the serial cultures. The proportion of methane as a percentage of the headspace gas is indicated for various enrichment substrates at the end of week 1 () through to the end of week 6 (□).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3815384&req=5

fig02: Methane accumulation as monitored at the end of the 7-day consecutive incubation period for each of the serial cultures. The proportion of methane as a percentage of the headspace gas is indicated for various enrichment substrates at the end of week 1 () through to the end of week 6 (□).
Mentions: Stable methanogenic cultures were established with structural polymers and storage polymers including mannitol (Fig. 2) confirming that the ability to catabolize a wide range of seaweed carbohydrates was present in the initial ruminal population. Although fucoidan was catabolized less effectively than other polysaccharides its utilization was also associated with consistent methane formation.

Bottom Line: Only nine isolates out of 65 utilized >90% of the polysaccharide they were isolated on.The nine isolates (eight Prevotella spp. and one Clostridium butyricum) utilized whole Laminaria hyperborea extract and a range of seaweed polysaccharides, including alginate (seven out of nine isolates), laminarin and carboxymethylcellulose (eight out of nine isolates); while two out of nine isolates additionally hydrolysed fucoidan to some extent.Crude enzyme extracts from three of the isolates studied further had diverse glycosidases and polysaccharidase activities; particularly against laminarin and alginate (two isolates were shown to have alginate lyase activity) and notably fucoidan and carageenan (one isolate).

View Article: PubMed Central - PubMed

Affiliation: Department of Biological and Biomedical Sciences, School of Health and Life Sciences, Glasgow Caledonian University, Cowcaddens Road, Glasgow, G4 0BA, UK.

Show MeSH