The potential of bacteria isolated from ruminal contents of seaweed-eating North Ronaldsay sheep to hydrolyse seaweed components and produce methane by anaerobic digestion in vitro.
Bottom Line: Only nine isolates out of 65 utilized >90% of the polysaccharide they were isolated on.The nine isolates (eight Prevotella spp. and one Clostridium butyricum) utilized whole Laminaria hyperborea extract and a range of seaweed polysaccharides, including alginate (seven out of nine isolates), laminarin and carboxymethylcellulose (eight out of nine isolates); while two out of nine isolates additionally hydrolysed fucoidan to some extent.Crude enzyme extracts from three of the isolates studied further had diverse glycosidases and polysaccharidase activities; particularly against laminarin and alginate (two isolates were shown to have alginate lyase activity) and notably fucoidan and carageenan (one isolate).
Affiliation: Department of Biological and Biomedical Sciences, School of Health and Life Sciences, Glasgow Caledonian University, Cowcaddens Road, Glasgow, G4 0BA, UK.Show MeSH
Mentions: Stable methanogenic cultures were established with structural polymers and storage polymers including mannitol (Fig. 2) confirming that the ability to catabolize a wide range of seaweed carbohydrates was present in the initial ruminal population. Although fucoidan was catabolized less effectively than other polysaccharides its utilization was also associated with consistent methane formation.
Affiliation: Department of Biological and Biomedical Sciences, School of Health and Life Sciences, Glasgow Caledonian University, Cowcaddens Road, Glasgow, G4 0BA, UK.