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Isolation and characterization of Cupriavidus basilensis HMF14 for biological removal of inhibitors from lignocellulosic hydrolysate.

Wierckx N, Koopman F, Bandounas L, de Winde JH, Ruijssenaars HJ - Microb Biotechnol (2009)

Bottom Line: The bacterium Cupriavidus basilensis HMF14 was isolated from enrichment cultures with HMF as the sole carbon source and was found to metabolize many of the toxic constituents of lignocellulosic hydrolysate including furfural, HMF, acetate, formate and a host of aromatic compounds.Remarkably, this microorganism does not grow on the most abundant sugars in lignocellulosic hydrolysates: glucose, xylose and arabinose.In addition, C. basilensis HMF14 can produce polyhydroxyalkanoates.

View Article: PubMed Central - PubMed

Affiliation: TNO Quality of Life, Department of Bioconversion, Julianalaan 67, 2628 BC, Delft, the Netherlands. N.J.P.Wierckx@tudelft.nl

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Detection of PHA in cultures of C. basilensis HMF14 in minimal medium with 120 mM acetate. Left: Phase contrast image. Middle: Fluorescence microscopic image of the same slide stained with Nile Blue A. Right: Overlay of the two previous images.
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f1: Detection of PHA in cultures of C. basilensis HMF14 in minimal medium with 120 mM acetate. Left: Phase contrast image. Middle: Fluorescence microscopic image of the same slide stained with Nile Blue A. Right: Overlay of the two previous images.

Mentions: The genus Cupriavidus is well known for its ability to efficiently produce PHA (Yu and Stahl, 2008; Reinecke and Steinbuchel, 2009). In order to verify PHA production by the newly isolated C. basilensis HMF14, this strain was cultivated in minimal medium with acetate as a carbon source. Fluorescence microscopic analysis showed PHA granules within the cells of C. basilensis (Fig. 1).


Isolation and characterization of Cupriavidus basilensis HMF14 for biological removal of inhibitors from lignocellulosic hydrolysate.

Wierckx N, Koopman F, Bandounas L, de Winde JH, Ruijssenaars HJ - Microb Biotechnol (2009)

Detection of PHA in cultures of C. basilensis HMF14 in minimal medium with 120 mM acetate. Left: Phase contrast image. Middle: Fluorescence microscopic image of the same slide stained with Nile Blue A. Right: Overlay of the two previous images.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815375&req=5

f1: Detection of PHA in cultures of C. basilensis HMF14 in minimal medium with 120 mM acetate. Left: Phase contrast image. Middle: Fluorescence microscopic image of the same slide stained with Nile Blue A. Right: Overlay of the two previous images.
Mentions: The genus Cupriavidus is well known for its ability to efficiently produce PHA (Yu and Stahl, 2008; Reinecke and Steinbuchel, 2009). In order to verify PHA production by the newly isolated C. basilensis HMF14, this strain was cultivated in minimal medium with acetate as a carbon source. Fluorescence microscopic analysis showed PHA granules within the cells of C. basilensis (Fig. 1).

Bottom Line: The bacterium Cupriavidus basilensis HMF14 was isolated from enrichment cultures with HMF as the sole carbon source and was found to metabolize many of the toxic constituents of lignocellulosic hydrolysate including furfural, HMF, acetate, formate and a host of aromatic compounds.Remarkably, this microorganism does not grow on the most abundant sugars in lignocellulosic hydrolysates: glucose, xylose and arabinose.In addition, C. basilensis HMF14 can produce polyhydroxyalkanoates.

View Article: PubMed Central - PubMed

Affiliation: TNO Quality of Life, Department of Bioconversion, Julianalaan 67, 2628 BC, Delft, the Netherlands. N.J.P.Wierckx@tudelft.nl

Show MeSH
Related in: MedlinePlus