What heat is telling us about microbial conversions in nature and technology: from chip- to megacalorimetry.
Bottom Line: New calorimeter developments overcome the weaknesses of conventional devices, which hitherto limited the full exploitation of this powerful analytical tool.Calorimetric systems can be integrated easily into natural and technological systems of interest.They are potentially suited for high-throughput measurements and are robust enough for field deployment.
Affiliation: UFZ, Helmholtz Centre for Environmental Research, Department of Environmental Microbiology, Permoserstr. 15, 04318 Leipzig, Germany. Thomas.email@example.comShow MeSH
Related in: MedlinePlus
Mentions: Halomonas elongata maintains an osmotic balance by synthesis and accumulation of 5,6‐tetrahydro‐2‐methyl‐4‐pyrimidinecarboxylic acid (ectoine). It was continuously cultured in a fermenter calorimeter in which the salt concentration was increased linearly. All other factors influencing growth including D and S0 were kept constant. Equation 4 predicts there will be a constant heat production rate, because it does not account for the energy expenditure of osmoadaptation. However, Fig. 3 shows that heat production rate in fact decreased in two phases, a linear first one followed by a more drastic drop. Whilst the sharp reduction in heat production rate during the second phase reflected the increasing residual substrate, the initial linear decrease could only be explained assuming a changed growth stoichiometry and consequently changed metabolic fluxes.
Affiliation: UFZ, Helmholtz Centre for Environmental Research, Department of Environmental Microbiology, Permoserstr. 15, 04318 Leipzig, Germany. Thomas.firstname.lastname@example.org