What heat is telling us about microbial conversions in nature and technology: from chip- to megacalorimetry.
Bottom Line: New calorimeter developments overcome the weaknesses of conventional devices, which hitherto limited the full exploitation of this powerful analytical tool.Calorimetric systems can be integrated easily into natural and technological systems of interest.They are potentially suited for high-throughput measurements and are robust enough for field deployment.
Affiliation: UFZ, Helmholtz Centre for Environmental Research, Department of Environmental Microbiology, Permoserstr. 15, 04318 Leipzig, Germany. Thomas.firstname.lastname@example.orgShow MeSH
Related in: MedlinePlus
Mentions: A weakness of the Higuera‐Guisset calorimeter is the relatively huge chamber volume of 600 µl. The chip‐calorimeter in one authors' lab fulfils the technical requirements for bioprocess control (2 mW l−1) with a 6 µl measurement chamber for most economic sample consumption (Lerchner et al., 2008a). Figure 2A shows substrate degradation and the formation characteristics of biomass and side‐products. The heat production rate can be calculated in advance from such data. The comparison of the calculated with the measured data (Fig. 2B) demonstrates clearly that such a nano‐calorimeter works well even at low biomass concentrations (> 1 mM = 0.025 g l−1) (Maskow et al., 2006a).
Affiliation: UFZ, Helmholtz Centre for Environmental Research, Department of Environmental Microbiology, Permoserstr. 15, 04318 Leipzig, Germany. Thomas.email@example.com