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What heat is telling us about microbial conversions in nature and technology: from chip- to megacalorimetry.

Maskow T, Kemp R, Buchholz F, Schubert T, Kiesel B, Harms H - Microb Biotechnol (2009)

Bottom Line: New calorimeter developments overcome the weaknesses of conventional devices, which hitherto limited the full exploitation of this powerful analytical tool.Calorimetric systems can be integrated easily into natural and technological systems of interest.They are potentially suited for high-throughput measurements and are robust enough for field deployment.

View Article: PubMed Central - PubMed

Affiliation: UFZ, Helmholtz Centre for Environmental Research, Department of Environmental Microbiology, Permoserstr. 15, 04318 Leipzig, Germany. Thomas.maskow@ufz.de

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Related in: MedlinePlus

Testing a chip‐calorimeter with suspended E. coli DH5α cells aerobically growing on glycerol. Points and lines represent measurements and results of the thermokinetic modelling, respectively, in (A) and (B). During the first phase (1 P) the strain grows on glycerol and produces acetate whereas in the second growth phase (2 P) acetate is used as sole source of carbon and energy (Maskow et al., 2006a).
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f2: Testing a chip‐calorimeter with suspended E. coli DH5α cells aerobically growing on glycerol. Points and lines represent measurements and results of the thermokinetic modelling, respectively, in (A) and (B). During the first phase (1 P) the strain grows on glycerol and produces acetate whereas in the second growth phase (2 P) acetate is used as sole source of carbon and energy (Maskow et al., 2006a).

Mentions: A weakness of the Higuera‐Guisset calorimeter is the relatively huge chamber volume of 600 µl. The chip‐calorimeter in one authors' lab fulfils the technical requirements for bioprocess control (2 mW l−1) with a 6 µl measurement chamber for most economic sample consumption (Lerchner et al., 2008a). Figure 2A shows substrate degradation and the formation characteristics of biomass and side‐products. The heat production rate can be calculated in advance from such data. The comparison of the calculated with the measured data (Fig. 2B) demonstrates clearly that such a nano‐calorimeter works well even at low biomass concentrations (> 1 mM = 0.025 g l−1) (Maskow et al., 2006a).


What heat is telling us about microbial conversions in nature and technology: from chip- to megacalorimetry.

Maskow T, Kemp R, Buchholz F, Schubert T, Kiesel B, Harms H - Microb Biotechnol (2009)

Testing a chip‐calorimeter with suspended E. coli DH5α cells aerobically growing on glycerol. Points and lines represent measurements and results of the thermokinetic modelling, respectively, in (A) and (B). During the first phase (1 P) the strain grows on glycerol and produces acetate whereas in the second growth phase (2 P) acetate is used as sole source of carbon and energy (Maskow et al., 2006a).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815370&req=5

f2: Testing a chip‐calorimeter with suspended E. coli DH5α cells aerobically growing on glycerol. Points and lines represent measurements and results of the thermokinetic modelling, respectively, in (A) and (B). During the first phase (1 P) the strain grows on glycerol and produces acetate whereas in the second growth phase (2 P) acetate is used as sole source of carbon and energy (Maskow et al., 2006a).
Mentions: A weakness of the Higuera‐Guisset calorimeter is the relatively huge chamber volume of 600 µl. The chip‐calorimeter in one authors' lab fulfils the technical requirements for bioprocess control (2 mW l−1) with a 6 µl measurement chamber for most economic sample consumption (Lerchner et al., 2008a). Figure 2A shows substrate degradation and the formation characteristics of biomass and side‐products. The heat production rate can be calculated in advance from such data. The comparison of the calculated with the measured data (Fig. 2B) demonstrates clearly that such a nano‐calorimeter works well even at low biomass concentrations (> 1 mM = 0.025 g l−1) (Maskow et al., 2006a).

Bottom Line: New calorimeter developments overcome the weaknesses of conventional devices, which hitherto limited the full exploitation of this powerful analytical tool.Calorimetric systems can be integrated easily into natural and technological systems of interest.They are potentially suited for high-throughput measurements and are robust enough for field deployment.

View Article: PubMed Central - PubMed

Affiliation: UFZ, Helmholtz Centre for Environmental Research, Department of Environmental Microbiology, Permoserstr. 15, 04318 Leipzig, Germany. Thomas.maskow@ufz.de

Show MeSH
Related in: MedlinePlus