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Substrate-dependent transcriptomic shifts in Pelotomaculum thermopropionicum grown in syntrophic co-culture with Methanothermobacter thermautotrophicus.

Kato S, Kosaka T, Watanabe K - Microb Biotechnol (2009)

Bottom Line: As it thrives on a very small energy conserved by propionate oxidation under syntrophic association with a methanogen, its catabolic pathways and regulatory mechanisms are of biological interest.Expression of the central catabolic pathway (the propionate-oxidizing methylmalonyl-CoA pathway) was found to be substrate-dependent and was largely stimulated when P. thermopropionicum was grown on propionate and lactate.These results revealed that P. thermopropionicum has complex regulatory mechanisms that alter its metabolism in response to the syntrophic partner and growth substrates.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Applied Microbiology, Marine Biotechnology Institute, Kamaishi, Iwate 026-0001, Japan.

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Comparison of qRT‐PCR and microarray data. Filled diamond, succinate dehydrogenase cytochrome b subunit (sdhC, PTH_1016); open circle, fumarase N‐terminal domain (mmcB, PTH_1356); open triangle, methylmalonyl‐CoA mutase N‐terminal domain (mmcE, PTH_1361); filled square, predicted lactate dehydrogenase (glcD, PTH_2230); asterisk, malic enzyme (sfcA, PTH_2899). The approximation curve and correlation coefficient (r) are given.
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f2: Comparison of qRT‐PCR and microarray data. Filled diamond, succinate dehydrogenase cytochrome b subunit (sdhC, PTH_1016); open circle, fumarase N‐terminal domain (mmcB, PTH_1356); open triangle, methylmalonyl‐CoA mutase N‐terminal domain (mmcE, PTH_1361); filled square, predicted lactate dehydrogenase (glcD, PTH_2230); asterisk, malic enzyme (sfcA, PTH_2899). The approximation curve and correlation coefficient (r) are given.

Mentions: Five genes (listed in the legend for Fig. 2) were selected for quantitative real‐time RT‐PCR (qRT‐PCR) analysis to examine if differential expression levels determined from the microarray data were supported by qRT‐PCR. Gene expression levels determined by qRT‐PCR (copies per ng of P. thermopropionicum RNA) in each syntrophic co‐culture sample were compared to those under the reference condition (P. thermopropionicum monoculture), and relative levels were plotted against the mean relative expression levels of corresponding genes determined by the microarray experiment (Fig. 2). This analysis demonstrated that qRT‐PCR and microarray data were consistent; the data were correlated well (r = 0.98) and had a slope that approached the unity (0.95).


Substrate-dependent transcriptomic shifts in Pelotomaculum thermopropionicum grown in syntrophic co-culture with Methanothermobacter thermautotrophicus.

Kato S, Kosaka T, Watanabe K - Microb Biotechnol (2009)

Comparison of qRT‐PCR and microarray data. Filled diamond, succinate dehydrogenase cytochrome b subunit (sdhC, PTH_1016); open circle, fumarase N‐terminal domain (mmcB, PTH_1356); open triangle, methylmalonyl‐CoA mutase N‐terminal domain (mmcE, PTH_1361); filled square, predicted lactate dehydrogenase (glcD, PTH_2230); asterisk, malic enzyme (sfcA, PTH_2899). The approximation curve and correlation coefficient (r) are given.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815365&req=5

f2: Comparison of qRT‐PCR and microarray data. Filled diamond, succinate dehydrogenase cytochrome b subunit (sdhC, PTH_1016); open circle, fumarase N‐terminal domain (mmcB, PTH_1356); open triangle, methylmalonyl‐CoA mutase N‐terminal domain (mmcE, PTH_1361); filled square, predicted lactate dehydrogenase (glcD, PTH_2230); asterisk, malic enzyme (sfcA, PTH_2899). The approximation curve and correlation coefficient (r) are given.
Mentions: Five genes (listed in the legend for Fig. 2) were selected for quantitative real‐time RT‐PCR (qRT‐PCR) analysis to examine if differential expression levels determined from the microarray data were supported by qRT‐PCR. Gene expression levels determined by qRT‐PCR (copies per ng of P. thermopropionicum RNA) in each syntrophic co‐culture sample were compared to those under the reference condition (P. thermopropionicum monoculture), and relative levels were plotted against the mean relative expression levels of corresponding genes determined by the microarray experiment (Fig. 2). This analysis demonstrated that qRT‐PCR and microarray data were consistent; the data were correlated well (r = 0.98) and had a slope that approached the unity (0.95).

Bottom Line: As it thrives on a very small energy conserved by propionate oxidation under syntrophic association with a methanogen, its catabolic pathways and regulatory mechanisms are of biological interest.Expression of the central catabolic pathway (the propionate-oxidizing methylmalonyl-CoA pathway) was found to be substrate-dependent and was largely stimulated when P. thermopropionicum was grown on propionate and lactate.These results revealed that P. thermopropionicum has complex regulatory mechanisms that alter its metabolism in response to the syntrophic partner and growth substrates.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Applied Microbiology, Marine Biotechnology Institute, Kamaishi, Iwate 026-0001, Japan.

Show MeSH
Related in: MedlinePlus