Substrate-dependent transcriptomic shifts in Pelotomaculum thermopropionicum grown in syntrophic co-culture with Methanothermobacter thermautotrophicus.
Bottom Line: As it thrives on a very small energy conserved by propionate oxidation under syntrophic association with a methanogen, its catabolic pathways and regulatory mechanisms are of biological interest.Expression of the central catabolic pathway (the propionate-oxidizing methylmalonyl-CoA pathway) was found to be substrate-dependent and was largely stimulated when P. thermopropionicum was grown on propionate and lactate.These results revealed that P. thermopropionicum has complex regulatory mechanisms that alter its metabolism in response to the syntrophic partner and growth substrates.
Affiliation: Laboratory of Applied Microbiology, Marine Biotechnology Institute, Kamaishi, Iwate 026-0001, Japan.Show MeSH
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Mentions: Five genes (listed in the legend for Fig. 2) were selected for quantitative real‐time RT‐PCR (qRT‐PCR) analysis to examine if differential expression levels determined from the microarray data were supported by qRT‐PCR. Gene expression levels determined by qRT‐PCR (copies per ng of P. thermopropionicum RNA) in each syntrophic co‐culture sample were compared to those under the reference condition (P. thermopropionicum monoculture), and relative levels were plotted against the mean relative expression levels of corresponding genes determined by the microarray experiment (Fig. 2). This analysis demonstrated that qRT‐PCR and microarray data were consistent; the data were correlated well (r = 0.98) and had a slope that approached the unity (0.95).
Affiliation: Laboratory of Applied Microbiology, Marine Biotechnology Institute, Kamaishi, Iwate 026-0001, Japan.