Plasmid addiction systems: perspectives and applications in biotechnology.
Bottom Line: Plasmid-free cells lead to losses in the entire product recovery and decrease the profitability of the whole process.However, not every system has reached a full applicable state.This review compares most known addiction systems and is focusing on biotechnical applications.
Affiliation: Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität, D-48149 Münster, Germany.Show MeSH
Related in: MedlinePlus
Mentions: Another system based on the unique par locus, which was found in Enterococcus faecalis, is so far the only post‐segregational‐killing system detected in Gram‐positive bacteria. The par locus consists of two genes which encode the 210‐nucleotide antisense RNA, RNA II and the 63‐nucleotide toxin‐encoding RNA I (fst) (Greenfield et al., 2000). Because their sequences are complementary to each other, RNA II is capable of regulating the transcription of RNA I and suppresses fst translation (Weaver et al., 1996). The par toxin, Fst, is activated by removal of RNA II when the plasmid is lost (Weaver et al., 2004). Figure 2 describes the par system as a representative of the antisense RNA‐regulated systems.
Affiliation: Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität, D-48149 Münster, Germany.