Interkingdom adenosine signal reduces Pseudomonas aeruginosa pathogenicity.
Bottom Line: Since the adenosine metabolite inosine did not affect biofilm formation and since a mutant unable to metabolize adenosine behaved like the wild-type strain, adenosine metabolism is not required to reduce pathogenicity.To provide insights into how adenosine reduces the virulence of P. aeruginosa, a whole-transcriptome analysis was conducted which revealed that adenosine addition represses genes similar to an iron-replete condition; however, adenosine did not directly bind Fur.Therefore, adenosine decreases P. aeruginosa pathogenicity as an interkingdom signal by causing genes related to iron acquisition to be repressed.
Affiliation: Department of Chemical Engineering, Texas A & M University, College Station, TX 77843-3122, USA.Show MeSH
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Mentions: The addition of 10 mM adenosine decreased the specific growth rate of PA14 by 25% (1.01 ± 0.06 h−1 versus 1.35 ± 0.01 h−1 for no adenosine) in LB medium. More significantly, 10 mM adenosine nearly abolished static biofilm formation in 96‐well plates (25‐fold decrease) (Fig. 1A). Similarly, adenosine reduced biofilm formation by 2.5‐fold in M9 glucose medium (Fig. 1A). We also investigated whether adenosine could induce biofilm dispersal. However, no significant effect was observed by the addition of adenosine.
Affiliation: Department of Chemical Engineering, Texas A & M University, College Station, TX 77843-3122, USA.